Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus subtilis m406 and its application in the preparation of bacteriocin and cellulase

A technology of Bacillus subtilis and cellulase, which is applied in the field of microorganisms, can solve problems such as unfavorable fermentation, reduce oxygen dissolution in the medium, and achieve the effect of preventing drug resistance and improving nutritional value

Active Publication Date: 2020-11-06
HUAZHONG AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inoculating Bacillus subtilis into it and standing it for fermentation will often form a layer of bacteria film on the surface of the medium, which will reduce the dissolution of oxygen in the medium. During the suspension culture process, bacteria will settle at the bottom, which is not conducive to the progress of fermentation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis m406 and its application in the preparation of bacteriocin and cellulase
  • Bacillus subtilis m406 and its application in the preparation of bacteriocin and cellulase
  • Bacillus subtilis m406 and its application in the preparation of bacteriocin and cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Bacillus subtilis (Bacillus subtilis) M406 strain isolation and identification

[0073] 1. Isolation of Bacillus subtilis M406 strain

[0074] 1.1 Separation

[0075] Fresh feces from adult commercial pigs and Meishan pigs were collected from a boutique pig farm of Huazhong Agricultural University, and placed in sterile tubes; pig manure was diluted with sterile PBS, and 10 -3 ~10 -6 100 μl each of the 4 dilutions of bacterial solution was spread on the primary screening medium plate, and cultured upside down at 37°C for 1d ( figure 1 ). Among them, the primary screening medium: CMC-Na 10g, Na 2 HPO 4 2.5g, KH 2 PO 4 1.5g, 1g peptone, 0.5g yeast powder, 15g agar, distilled water to 1L.

[0076] 1.2 Initial screening:

[0077] Select a single colony with good growth and inoculate it on the primary screening medium plate (the number of the single colony isolated from commercial pigs starts with "S", and the number of the single colony isolated from M...

Embodiment 2

[0088] 1. Identification of antibacterial effect of Bacillus subtilis M406

[0089] Inoculate Bacillus subtilis (Bacillus subtilis) M406 in LB medium at 1% inoculum size, culture at 37°C with shaking at 200r / min for 18 hours, take the culture solution and centrifuge at 12000r / min for two minutes, and pass the supernatant through a 0.22μm filter Then, the diameter of the inhibition zone was measured by punching method. Three replicate experiments were performed for each strain.

[0090]It was found that Bacillus subtilis (Bacillus subtilis) M406 culture solution has antibacterial effect on G+ bacteria S.aureus, S.suis, G- bacteria E.coli, S.typhimurium. S.aureus and S.suis are common bacteria causing infection, while E.coli and S.typhimurium are important causes of diarrhea in piglets.

[0091] This shows that the Bacillus subtilis (Bacillus subtilis) M406 culture solution can inhibit the growth of E.coli and S.typhimurium.

[0092] 2. Determination of the properties of anti...

Embodiment 3

[0100] 1. Preparation of Bacteriocin

[0101] The method utilizing bacillus subtilis (Bacillus subtilis) M406 to prepare bacteriocin comprises the following steps:

[0102] 1) Strain activation: Inoculate the Bacillus subtilis M406 strain on LA solid medium and culture it at 30-37°C for 12-24 hours to obtain a single colony; wherein,

[0103] The concentration of LA solid medium is composed of: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 15g / L;

[0104] 2) Seed solution preparation: pick a single colony and inoculate it in LB liquid medium, and cultivate it for 5-24 hours at a temperature of 30-37°C and a shaker speed of 150-220r / min until the strain reaches the mid-logarithmic growth stage. Obtain seed liquid; Wherein, LB liquid medium formula: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L;

[0105] 3) Liquid shake flask fermentation: put the seed liquid into the sterilized bacteriocin-producing medium, culture and ferment for 12-24 hours at a temperature of 30-37...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bacillus subtilis M406 and its application in preparing bacteriocin and cellulase. Bacillus subtilis (Bacillus subtilis) M406 strain, its preservation number is CCTCC NO: M 2018234. The present invention focuses on one trait and supplements multiple traits to screen out a strain of probiotics, which is Bacillus subtilis M406, which improves the use value of the probiotics. The antibacterial active substance produced by the bacillus subtilis M406 can inhibit various harmful bacteria including S.typhimurium, Salmonella enteritidis, S.aureus, E.coli and Listeria monocytogenes in the intestinal tract of animals. The probiotics can ferment various sugars to produce acid and lower the pH value of intestinal juice. The bacillus subtilis M406 produces cellulase, which can effectively degrade structural carbohydrates in roughage, convert part of NSP into soluble sugar, fat, protein and amino acid components, and improve the nutritional value of roughage.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis M406 and its application in the preparation of bacteriocin and cellulase. Background technique [0002] Bacillus, G + Bacteria, 0.7-0.8μm×1.0-1.5μm, straight or nearly straight rod-shaped bacteria, mostly motile, flagella lateral, strictly aerobic or facultatively anaerobic. Heterotrophic, aerobic metabolism, fermentative metabolism or both, some can reduce nitrate, most produce catalase. When in a negative environment, Bacillus endogenous spores, and there is no more than one spore in a sporangia, and the spore is the dormant state of Bacillus. The spores are highly stress-resistant and can withstand temperature, dryness, acid and alkali, disinfectants and various unfavorable factors. The spores will re-transform into vegetative cells under suitable conditions for growth and reproduction. Some Bacillus can produce a variety of digestive enzymes, ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C12N9/42A61K38/16A61P31/04C12R1/125
CPCA61K38/164A61P31/04C12N9/2437C12P21/00C12N1/205C12R2001/125Y02A50/30
Inventor 晏向华王新锴胡军侯奇良牛耀嵘
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com