Solanum muricatum Ait acidic polysaccharide and purification method and application thereof

A technology of acidic polysaccharide and purification method, applied in the direction of antitoxin, drug combination, etc., can solve the problem that the research of cantaloupe and eggplant has not been reported yet.

Active Publication Date: 2019-01-08
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on muskmelon and eggplant mainly focuses on various solvent extracts and small molecular compounds, and the research on polysaccharides in muskmelon and eggplant has not been reported yet.

Method used

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  • Solanum muricatum Ait acidic polysaccharide and purification method and application thereof
  • Solanum muricatum Ait acidic polysaccharide and purification method and application thereof
  • Solanum muricatum Ait acidic polysaccharide and purification method and application thereof

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Embodiment 1

[0047] see figure 1 , the purification method of muskmelon eggplant acidic polysaccharide SMP-3a comprises: (one) crude polysaccharide extraction and (two) two steps of separation and purification, wherein,

[0048] (1) The crude polysaccharide extraction process is:

[0049] (1) Raw material pretreatment: select fresh muskmelon eggplant, wash it with water, slice it, put it in a blast drying oven, dry it at a constant temperature of 50°C, grind it into powder with a pulverizer, and pass it through a 60-mesh sieve for later use.

[0050] (2) Impurity removal: the above melon eggplant powder was extracted with 90% ethanol under reflux at 90°C for 6 hours to remove lipids, pigments, monosaccharides and small molecular substances, and dried for later use.

[0051] (3) Hot water extraction: Soak the impurity-removed muskmelon and eggplant powder in water, extract at 80°C for 4 hours, filter to obtain the filtrate, and repeat the extraction of the filter residue twice, combine the...

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Abstract

The invention discloses a Solanum muricatum Ait acidic polysaccharide and a purification method and application thereof. The acidic polysaccharide is a homogeneous acidic heteropolysaccharide, the chemical structure thereof contains a pyran ring and a uronic acid, and the acidic polysaccharide is composed of rhamnose, arabinose, galactose, glucose and monosaccharide of galacturonic acid. The method comprises the following steps: crude polysaccharide extraction of Solanum muricatum Ait by (1) drying, pulverizing and impurity removal and (2) water extraction by alcohol sedimentation to obtain awater extraction crude polysaccharide; and separation and purification on the water extraction crude polysaccharide by (i) protein removal, dialysis and vacuum freeze-drying, (ii) macroporous resin adsorption, (iii) anion exchange chromatography purification, and/or (iv) gel column chromatography purification. The obtained Solanum muricatum Ait acidic polysaccharide has good antioxidant capacity and can be used for food, cosmetic and pharmaceutical industries as a novel antioxidant.

Description

technical field [0001] The invention relates to the technical field of biological purification, in particular to a muskmelon solanum acidic polysaccharide with antioxidative effect and its purification method and application. Background technique [0002] Melon eggplant (Solanum muricatum Ait) is a perennial herb of the Solanaceae family Solanaceae. It is native to the northern foothills of the Andes in South America. It was introduced into China in the 1980s and widely distributed in Yunnan, Gansu and other places. It is a pillar industry for local farmers to increase their income. In-depth research on it can promote the comprehensive development and utilization of cantaloupe and eggplant, and improve the economic benefits of cantaloupe and eggplant. Cantaloupe eggplant is also known as ginseng fruit, muskmelon pear, colorful fruit, fragrant eggplant, fragrant pear, etc. The fruit is pear-shaped, oval, milky-yellow berry, the peel is green when immature, and turns golden ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
CPCA61P39/06C08B37/0003C08B37/006
Inventor 吴文惠岳恒许倩倩许剑锋苏攀峰马子宾王锋
Owner SHANGHAI OCEAN UNIV
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