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Low molecular weight fucoidan and application thereof in preparing cosmetics

A technology of fucoidan and low molecular weight, applied in cosmetics, cosmetic preparations, medical preparations containing active ingredients, etc., can solve problems such as human health damage, achieve good repeatability, good market application prospects, and simple process Effect

Active Publication Date: 2018-12-21
青岛创通生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many whitening products in the world mainly use the method of adding arbutin to inhibit the activity of tyrosinase, but this method will cause damage to human health while inhibiting tyrosinase

Method used

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  • Low molecular weight fucoidan and application thereof in preparing cosmetics
  • Low molecular weight fucoidan and application thereof in preparing cosmetics
  • Low molecular weight fucoidan and application thereof in preparing cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of described low molecular weight fucoidanase

[0028] (1) Enzyme-producing microorganisms

[0029] The present invention first prepares fucoidanase, and the microorganism used is Flavobacteriaceaesp. RC2-3mut, which has been preserved at present, and the depository unit: General Microorganism Center (CGMCC) of China Microorganism Culture Preservation Management Committee , Deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, deposit date: November 03, 2017, deposit number: CGMCC No.14855. The colony of the strain RC2-3mut is yellow, round and protruding, with neat edges and no movement. The cells are negative for Gram staining, negative for contact enzymes, and positive for oxidase. They grow in seawater and in 1% NaCl.

[0030] (2) Enzyme production method of RC2-3mut

[0031] Inoculate the microorganism Flavobacteriaceae sp. RC2-3mut strain in a liquid medium, and...

Embodiment 2

[0035] Embodiment 2, the preparation of fucoidan

[0036] The preparation method of the low-molecular-weight fucoidan of the present invention is as follows: dissolve the freeze-dried fucoidan enzyme in distilled water, mix it with a fucoidan solution with a mass ratio of 0.2%-2%, and place it at 27°C and 120rpm After 27 hours of enzymolysis in a water-bath shaker, place in a water bath at 100°C for 20 minutes, centrifuge at 8,000 rpm for 10 minutes, and take the supernatant. The supernatant was fractionated into low-molecular-weight fucoidan of different molecular weight segments (the molecular weight segments were less than 5kDa, 5-10kDa, 10-50kDa, 50-100kDa, greater than 100kDa) with the Vivaflow ultrafiltration system.

Embodiment 3

[0037] Example 3, low molecular weight fucoidan has whitening activity

[0038] (1) Tyrosinase inhibitory activity

[0039] The tyrosinase inhibitory activity was determined according to the method in Table 1. Add L-tyrosine, sample, PBS buffer, and tyrosinase to the tubes numbered A1, A2, B1, and B2 in sequence, and place each tube in a water bath shaker at 37°C for accurate reaction for 15 minutes. Bath 10min to stop the reaction, measure the absorbance at 475nm, and calculate the inhibition rate. The formula for calculating the inhibition rate is:

[0040]

[0041] A1 blank group has enzyme system absorbance; A2 blank group has no enzyme system absorbance; B1 sample group has enzyme system absorbance; B2 sample group has no enzyme system absorbance.

[0042] Table 1 inhibition of tyrosinase activity sample amount

[0043]

[0044] Using the above method to measure the activity of fucoidan with different enzymatic hydrolysis time and different molecular weight segm...

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Abstract

The invention provides a low molecular weight fucoidan and application thereof in preparing cosmetics. Flavobacterium with preservation number of CGMCC NO. 14855 is utilized to produce fucoidan degrading enzyme after fermenting, and the fucoidan degrading enzyme is further utilized to degrade the fucoidin to obtain the low molecular weight fucoidan. The experimental results show that the low molecular weight fucoidan can significantly inhibit tyrosinase activity and has significant antioxidant activity. The low molecular weight fucoidan provided by the invention has the potential to be prepared into whitening and anti-aging cosmetics; and raw materials are derived from kelp, which is a pure natural extract, safe and non-irritating, and has good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a low molecular weight fucoidan and its application in the preparation of cosmetics. Background technique [0002] Fucoidan is a sulfated heteropolysaccharide of fucoidan, mainly composed of fucose, galactose, mannose and sulfate groups. Many literature studies have shown that fucoidan has a variety of biological activities, such as anticoagulant Blood, anti-tumor, anti-oxidation, anti-infection, etc., but because of its large molecular weight, complex chemical composition, and poor absorption, its application is limited. Degrading fucoidan with a large molecular mass into polysaccharides with a relatively small molecular mass can not only solve the above application problems, but also enhance its activity in some cases. The enzymatic hydrolysis method can specifically and selectively cut off the glycosidic bonds on the fucoidan chain, the reaction process is easy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04A61K8/73A61Q19/08A61Q19/02C12R1/20
CPCA61K8/73A61K2800/782A61K2800/85A61Q19/02A61Q19/08C12P19/04C12P19/14
Inventor 鲁勇王莹寇玲赟牛德军
Owner 青岛创通生物科技有限公司
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