Preparation method of K-carrageenan oligosaccharide having antioxidant activity
A carrageenan oligosaccharide, antioxidant activity technology, applied in biochemical equipment and methods, preparation of test samples, microorganism-based methods, etc. The preparation process is simple and fast, and the quality is stable.
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[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments.
[0030] A preparation method of K-carrageenan oligosaccharides with antioxidant activity specifically comprises:
[0031] One, the preparation of K-carrageenase
[0032] 1. Inoculate the screened Cellulophagalytica strain N5-2 in the activation medium, and culture it on a shaker at 20-30°C and 150-250rpm / min for 15-20h, and then activate it to obtain a seed solution.
[0033] 2. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 2-4%, and culture on a shaker at 20-30°C and 150-250rpm / min for 15-20h.
[0034] 3. Refrigerate and centrifuge the fermentation broth at 4000-5000r / min for 20min at 4°C, and take the supernatant after centrifugation, which is the crude enzyme solution.
[0035] 2. Preparation of K-carrageenan oligosaccha...
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