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A method for systematically discriminating biofilm activity in wastewater treatment systems

A treatment system and biofilm technology, which is applied in the field of systematic identification of biofilm activity in wastewater treatment systems, can solve the problems of incomplete biofilm activity measurement methods, and achieve intuitive results, intuitive analysis results, and high sensitivity.

Active Publication Date: 2020-08-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem that existing wastewater treatment biofilm activity determination methods cannot comprehensively and systematically reflect biofilm activity, the present invention provides a method for systematically identifying biofilm activity in wastewater treatment systems

Method used

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  • A method for systematically discriminating biofilm activity in wastewater treatment systems

Examples

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Comparison scheme
Effect test

Embodiment 1

[0057] In this example, the biofilm activity detection of an industrial wastewater aerobic unit annular packing (2.5 cm in diameter) is carried out. The influent water quality in this system is: pH: 7.20-7.85, chemical oxygen demand is 1200-2800mg / L, ammonia nitrogen concentration is 2.85-11.54mg / L, total nitrogen concentration is 64-79mg / L, and total phosphorus concentration is 12.8 ~28.1mg / L.

[0058] The specific implementation process of this example is:

[0059] 1) Sampling the biofilm of the target filler: put it in a 4°C sampling box, and bring it back to the laboratory for analysis within 2 hours;

[0060] 2) Analyze the target biofilm into biofilms of different binding forms, the specific steps are as follows:

[0061] a. Drain the water on the surface of the filler, and the draining time is 30s;

[0062] b. Select the diameter of the fixed tube to be 2.5cm;

[0063] c. Put the filler into the fixed tube, place a single filler in a single fixed tube, and repeat th...

Embodiment 2

[0076] In this example, the biofilm activity detection of the aerobic unit annular filler (2.5 cm in diameter) in a certain domestic sewage is carried out. The water quality in the system is: pH: 7.0-7.55, chemical oxygen demand 220-380mg / L, ammonia nitrogen concentration 38.5-61.54mg / L, total nitrogen concentration 67.2-79mg / L, total phosphorus concentration 3.8 ~8.1mg / L.

[0077] The specific implementation process of this example is:

[0078] 1) Sampling the biofilm of the target filler: put it in a 4°C sampling box, and bring it back to the laboratory for analysis within 2 hours;

[0079] 2) Analyze the target biofilm into biofilms of different binding forms, the specific steps are as follows:

[0080] a. Drain the water on the surface of the filler, and the draining time is 40s;

[0081] b. Select the diameter of the fixed tube to be 2.5cm;

[0082] c. Put the filler into the fixed tube, place a single filler in a single fixed tube, and repeat the fixed tube 15 times;...

Embodiment 3

[0095] In this example, the biofilm activity detection of the aerobic unit annular packing (2.5 cm in diameter) in wastewater from a industrial park is carried out. The water quality in the system is: pH: 7.30-8.05, chemical oxygen demand 2200-3400mg / L, ammonia nitrogen concentration 12.85-32.54mg / L, total nitrogen concentration 84-99mg / L, total phosphorus concentration 22.8 ~38.1mg / L.

[0096] The specific implementation process of this example is:

[0097] 1) Sampling the biofilm of the target filler: put it in a 4°C sampling box, and bring it back to the laboratory for analysis within 2 hours;

[0098] 2) Analyzing the target biofilm into biofilms of different binding forms, the specific steps are as follows:

[0099] a. Drain the water on the surface of the filler, and the draining time is 30s;

[0100] b. Select the diameter of the fixed tube to be 2.5cm;

[0101] c. Put the filler into the fixed tube, place a single filler in a single fixed tube, and repeat the fixed...

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Abstract

The invention discloses a method for systematically judging the activity of a biofilm in a sewage and wastewater treatment system, and belongs to the technical field of biofilm sewage treatment. Afterthe biofilm is sampled, the biofilm is spatially layered, and the contents of C6-HSL, C10-HSL and C12-HSL in the biofilm are measured; when the content of C10-HSL in the surface biofilm is less than2.2 ng / g, the surface biofilm is in a low activity state; when the content of C12-HSL in the loosely coupled biofilm is less than 14 ng / g, the biofilm of this layer is in a low activity state; and when the contents of C6-HSL, C10-HSL and C12-HSL in the tightly coupled biofilm are less than 28.6 ng / g, the biofilm of this layer is in a low activity state. After the spatial structure of the biofilm is analyzed, the activity of the biofilm is judged by using the characteristic signal substances in the wastewater treatment biofilm, so that the result guidance is strong, the accuracy of judging thestate of the sewage treatment biofilm can be significantly improved, and the operational reliability of the biofilm process for the sewage treatment system is improved.

Description

technical field [0001] The invention belongs to the technical field of biofilm wastewater treatment, and more specifically relates to a method for systematically discriminating biofilm activity in a wastewater treatment system. Background technique [0002] The biofilm process is one of the mainstream processes for wastewater biological treatment. Due to the large biomass and complex biological species of the biofilm process system, the impact load is resistant, the effluent water quality is stable, and the sludge age of microorganisms is long, which is conducive to the growth of microorganisms such as nitrifying bacteria. reproduce. The biofilm on the filler surface can form the following stages: macromolecule adhesion, reversible adhesion, irreversible adhesion, massive secretion of extracellular polymers, biofilm maturation stage and biofilm aging stage. Biofilm can form an aerobic and anaerobic microenvironment, which provides a good living environment for nitrogen and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/12C02F3/28
CPCC02F3/1268C02F3/2853C02F2209/36Y02W10/10
Inventor 任洪强王瑾丰丁丽丽许柯张宴刘秋菊
Owner NANJING UNIV
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