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Method for extracting small molecule mushroom polysaccharide with microelement-enriched deuterium-depleted water

A technology of trace elements and deuterium-depleted water, which is applied in the field of mushroom polysaccharide extraction, can solve the problems of mushroom polysaccharides with large molecular weight, low digestion and utilization rate, and difficult absorption, and achieve small polysaccharide molecules, high extraction rate, and good absorption Effect

Inactive Publication Date: 2019-01-04
ANHUI GUOKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The macromolecular mushroom polysaccharide has a large molecular weight and also contains soluble cellulose, which is difficult for the human body to absorb and has low digestion and utilization rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for extracting small-molecule mushroom polysaccharides from deuterium-enriched water comprising the following steps:

[0034] 1) Dried shiitake mushrooms were chopped and passed through a 100-mesh sieve, soaked in 12 times the mass of glacier water (deuterium-depleted water) for 5 hours, then heated to 90°C, and microwave-extracted for 1 hour under the condition of microwave power 500W.

[0035] 2) Centrifuge the extraction system in step 1), collect the extract, freeze at -18°C for 8 hours, dissolve at room temperature, then centrifuge, collect the supernatant, and freeze at -18°C for 6 hours , placed at room temperature to dissolve, then centrifuged to collect the supernatant, and then placed at -18 ° C for 4 hours, placed at room temperature to dissolve, and then centrifuged to collect the supernatant;

[0036] 3) Add sodium bicarbonate to the supernatant obtained in step 2), adjust the pH to 7, then add 4% trypsin of the supernatant mass, heat the water bat...

Embodiment 2

[0042] Using glacier water, according to the publication number 101481088A, the application number is 200910046130.8, and the method disclosed in the application title is "A device for preparing ultra-light water and a method for preparing ultra-light water" to prepare deuterium-depleted water with a deuterium abundance of 35 ppm.

[0043] A method for extracting small-molecule mushroom polysaccharides from deuterium-enriched water comprising the following steps:

[0044] 1) Dried shiitake mushrooms were chopped and passed through a 200-mesh sieve, soaked in 12 times the mass of deuterium-depleted water with a deuterium abundance of 35ppm for 4 hours, then heated to 95°C and microwave-extracted for 2 hours at a microwave power of 450W.

[0045] 2) Centrifuge the extraction system in step 1), collect the extract, freeze at -20°C for 5 hours, dissolve at room temperature, centrifuge, collect the supernatant, and freeze at -20°C for 6 hours , put it at room temperature to dissolv...

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Abstract

The invention provides a method for extracting small molecule mushroom polysaccharide with microelement-enriched deuterium-depleted water. By means of the low deuterium characteristic of glacier water, the glacier water serves as an extraction solvent, and due to the fact that the glacier is rich in microelement, dissolution and extraction of the mushroom polysaccharide are facilitated; in the extraction process, the deuterium-depleted water is heated, microwave extraction is cooperated, the extraction efficiency of the polysaccharide is improved conveniently, and the extraction rate of the polysaccharide is higher; after extraction, repeated freezing and thawing are conducted first, protein and other impurities are removed, the protein is further removed by means of trypsin, the polysaccharide acquisition rate is increased, final alcohol sedimentation is conducted once, and the protein can be efficiently removed; finally, trifluoroacetic acid is added, the mushroom polysaccharide is cut to be small molecule polysaccharide, human body absorption is facilitated, and health-care value is improved. The polysaccharide has the effects of inhibiting cancer cells, lowering blood fat, lowering blood glucose and the like. Compared with the prior art, according to the method, the polysaccharide extraction rate is high, few impurities exist, polysaccharide molecules are small, and human body absorption is facilitated.

Description

technical field [0001] The invention belongs to the field of extracting mushroom polysaccharides, and in particular relates to a method for extracting small-molecule mushroom polysaccharides from deuterium-rich water. Background technique [0002] Water that exists in nature is generally composed of 2 hydrogen atoms and 1 oxygen atom, but hydrogen atoms have 3 isotopes with different masses, hydrogen (H) and deuterium (D, heavy hydrogen) with atomic weights of 1, 2, and 3 respectively , Tritium (triotium). The one with lower deuterium content is deuterium-depleted water. [0003] The content of deuterium in natural water is about 150ppm, and the deuterium content of natural deuterium-depleted water (glacier water) is between 130ppm-145ppm. The water bound by D instead of H is heavy water. All natural water bodies on the earth contain deuterium, an isotope of hydrogen, which can inhibit the growth of cells, distort or break the genetic information of DNA, and make it diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003
Inventor 张斌徐伟凌中鑫
Owner ANHUI GUOKE BIOTECH CO LTD
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