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Application of dendrobium officinale extract in preparing product for preventing Alzheimer's Diseases

A technology for Alzheimer's disease and Dendrobium candidum, applied in the application field of products, can solve the problems of undisclosed related effects and effects, and achieve the effects of easy portability and storage, various forms, and high safety

Inactive Publication Date: 2019-01-04
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on phenanthrene in Dendrobium candidum. Among them, Chinese patent CN104069365A discloses the use of polyphenols in Dendrobium candidum to protect the liver and treat / prevent diabetes. Inflammation and other liver lesions, blood sugar regulation and weight loss effects can be used in the preparation of health food for liver protection and treatment / prevention of diabetes, but the above-mentioned active ingredients have not disclosed their related effects on liver lesions and diabetes
Chinese patent CN104258106B discloses a method for preparing the effective part of Dendrobium officinale. The effective part is mainly extracted from a high-concentration alcohol-water system to extract the fat-soluble components mainly bibenzyl, and the total polysaccharide of Dendrobium candidum prepared by water extraction and alcohol precipitation. Prepared by mixing, the above-mentioned effective parts have anti-oxidation and anti-tumor activities, but the patent does not disclose its related effects on oxidative stress and tumors
At present, there is no report on the research on the effect of phenanthrene compounds in Dendrobium officinale on Alzheimer's disease

Method used

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  • Application of dendrobium officinale extract in preparing product for preventing Alzheimer's Diseases
  • Application of dendrobium officinale extract in preparing product for preventing Alzheimer's Diseases
  • Application of dendrobium officinale extract in preparing product for preventing Alzheimer's Diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 Dendrobium officinale extract

[0046] Take 300g of Dendrobium candidum medicinal material, crush it, add 2.4L of 95% ethanol solution, extract in a water bath at 55°C for 2-3 times, each time for 40 minutes, combine the extracts, use a rotary evaporator to recover the solvent, and concentrate the extracts under reduced pressure. Utilize the AB-8 macroporous resin column to purify the above concentrated solution. The purification process is to use the AB-8 macroporous resin column to adsorb the above concentrated solution for 2 hours and then elute with distilled water (discard the distilled water for elution to obtain eluent), then use ethanol with a volume concentration of 60% to elute, collect the eluate obtained by eluting with a volume concentration of 60% ethanol and concentrate, dry to obtain the Dendrobium candidum extract, and use a UV spectrophotometer The content of phenanthrene compounds in the obtained Dendrobium officinale e...

Embodiment 2

[0047] Example 2 Anti-AD activity of Dendrobium officinale extract

[0048] Referring to the method of Solis G.M. (Solis G.M. et al., Journal of Visualized Experiments, 2011, 49(e2496):1-6), transfer the synchronized CL4176 nematodes (about 40-50) in the L1 phase to the Escherichia coli OP50 and drugs (10 μL of solvent was added to the blank control group, 10 μL of 500 μg / ml of Dendrobium candidum extract SH-F of Example 1 was added to the drug group, and 10 μL of 1000 μg / ml of Ginkgo biloba was added to the positive control group Extract Egb) on a 35mm nematode growth medium (NGM) plate, culture at a constant temperature of 16°C, transfer to a constant temperature culture at 23°C after 36 hours, and record it as 0 hour, and observe the paralysis of nematodes after 28 hours of cultivation , record the total amount of nematodes, the number of paralyzed or non-paralyzed numbers, and make statistics every hour until all the nematodes are paralyzed. GraphPad Prism 6 was used for ...

Embodiment 3

[0052] Example 3 Anti-AD mechanism of Dendrobium officinale extract

[0053] 1. Dendrobium officinale extract inhibits the toxicity of Aβ

[0054] The CL2331 strain nematode is a transgenic nematode whose body wall cells have been transformed with the human β-amyloid gene, and the gene is connected in series with the GFP green fluorescent protein gene, which can be induced to express at 23°C, and its expression can be detected by fluorescence microscopy quantity. This part of the experiment was carried out using CL2331 nematodes, and the steps were as follows: transfer the synchronized CL2331 nematodes (about 20-30) in the L4 phase to the place where E. Add 10 μL 500 μg / ml Dendrobium candidum extract SH-F of Example 1) on a 35 mm nematode growth medium (NGM) plate, incubate at a constant temperature at 20°C, after 48 h, transfer to a constant temperature culture at 23°C, and after 24 h The fluorescence intensity was detected under a fluorescence microscope, and Image J was u...

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Abstract

The invention provides application of a dendrobium officinale extract in preparing a product for preventing Alzheimer's Diseases. The product comprises a dendrobium officinale extract, wherein the dendrobium officinale extract comprises a phenolic compound; the weight of the phenolic compound in the dendrobium officinale extract accounts for 40% of the total weight of the dendrobium officinale extract or greater; the product is any one of medicines, healthcare products and functional foods. The invention discloses that the phenolic compound in dendrobium officinale is capable of remarkably retarding the paralysis time of CL4176 caenorhabditis elegans, remarkably inhibiting toxicity of Abeta proteins, in addition remarkably enhancing the anti-oxidation functions of bodies and reducing intake amounts and can be applied to prevention and treatment on Alzheimer's Diseases.

Description

technical field [0001] The invention relates to the field of medicine and health products, in particular to the application of an extract of Dendrobium candidum in the preparation of anti-Alzheimer's products. Background technique [0002] Alzheimer's disease (AD), commonly known as senile dementia, is a degenerative disease of the central nervous system with an insidious onset and a chronic progressive course. Neuropsychiatric symptoms such as neuropsychiatric disorders, cognitive dysfunction, personality changes, and language barriers can seriously affect social, occupational, and life functions. [0003] With the intensification of the aging population in our country, Alzheimer's disease has become an important issue of family burden and social medical insurance. According to statistics, as of 2017, the number of patients with Alzheimer's disease in my country has exceeded 10 million. Both the total number of patients and the growth rate are the first in the world. By 20...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P25/28A23L33/105
CPCA23V2002/00A61K36/8984A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23L33/105A61P25/28A23V2200/322A23V2250/21
Inventor 高益槐王红兵唐文波余映婷
Owner ALPHA FUJIAN BIOTECH
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