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Effective part of nutgall with anti-inflammatory effect and preparation method thereof

A technology with anti-inflammatory effect and effective parts, applied in the field of effective parts and its preparation, can solve problems such as unclearness, and achieve the effects of less environmental pollution, high yield and low cost

Pending Publication Date: 2019-01-04
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported in the literature that gallium has anti-inflammatory effects, it is still unclear which part is responsible

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The first step: choose gallate as medicinal material, pulverize, and pass through a 10-mesh sieve;

[0015] Step 2: Mix the powder of gallic medicinal material with 1 / 2 of the volume of water, decoct and extract once, 1 hour each time, combine the filtrates, concentrate and dry to prepare for subsequent separation;

[0016] The third step: use the polyamide chromatographic column to enrich the effective part of the sample obtained in the second step: elute with water to remove impurities, then elute with 20% ethanol, collect the eluate, concentrate and dry, and obtain the concentrated solution Or the dry solid is the gallic anti-inflammatory extract, the polyphenolic compound content in the extract is >80%, and the extract contains mono-O-galloyl glucose, di-O-galloyl glucose, digallic acid and gallic acid methyl ester.

Embodiment 2

[0018] The first step: choose gallate as the medicinal material, crush it, and pass through a 50-mesh sieve;

[0019] Step 2: Mix the powder of gallic medicinal material with 1 / 3 of the volume of water, decoct and extract twice for 2 hours each time, combine the filtrates, concentrate and dry to prepare for subsequent separation;

[0020] The third step: enrich the effective parts of the sample obtained in the second step by using a C18 chromatographic column: elute with 5% methanol to remove impurities, then elute with 20% methanol, collect the eluate, concentrate and dry to obtain The concentrated liquid or dried solid is the anti-inflammation extract of the gall, the content of the polyphenol compound in the extract is more than 80%, and the extract contains mono-O-galloyl glucose and di-O-galloyl glucose.

Embodiment 3

[0022] The first step: select gallate as medicinal material, crush, pass through 80-mesh sieve;

[0023] Step 2: Mix the powder of gallic medicinal material with 1 / 5 of the volume of water, decoct and extract 3 times, each time for 3 hours, combine the filtrate, concentrate and dry to prepare for subsequent separation;

[0024] The third step: use the polyamide chromatographic column to enrich the effective part of the sample obtained in the second step: remove impurities by eluting with water, collect the eluate after using 30% ethanol, concentrate and dry, and the obtained concentrated solution or dried The solid is the anti-inflammatory gallic extract, the polyphenol compound content in the extract is >80%, and the extract contains di-O-galloyl glucose, methyl gallate and tri-O-galloyl glucose.

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Abstract

The invention relates to an effective part of a nutgall with an anti-inflammatory effect and a preparation method thereof. A compound in the effective part of the nutgall with the anti-inflammatory effect shall include: 1, nutgall acyl glucose, 2, nutgall acyl glucose, digallic acid, methyl gallate, 3, one or more of nutgall acyl glucose. The preparation method comprises the following steps: grinding the nutgall, mixing the nutgall with water and decocting, mixing filtrates, concentrating and drying for subsequent separation; carrying out effective part enrichment on the obtained sample by using a C18 chromatographic column, a polyamide chromatographic column or a macroporous adsorption resin column, removing impurities, eluting by using ethanol or methanol, collecting eluent, concentrating and drying to obtain a concentrated solution or dried solid which is a nutgall anti-inflammatory extract. According to the invention, the effective part of the nutgall is determined through in-vitroand in-vivo pharmacological study after chromatographic separation is carried out. According to the invention, the preparation method is simple in process, reasonable in design and has small environmental pollution.

Description

technical field [0001] The invention relates to an anti-inflammatory effective part in gallaceae and a preparation method thereof. Background technique [0002] Galls are the galls produced by the larvae of Cynips gallae-tinctoriae Oliv. parasitizing on the twigs of Quercus infectoria Oliv. Contains 50-70% gallic tannin, 2-4% gallic acid, and gallic acid, resin, etc. Gallatin has a long history of medicinal use, which was first recorded in "Tang Materia Medica", followed by medical works "Haiyao Materia Medica", "Kaibao Materia Medica" and so on. "Modern Practical Chinese Medicine" also has records for the treatment of chronic bronchitis. Pharmacological studies have shown that gallic gall has various pharmacological activities such as anti-inflammatory, antibacterial, antifungal, antiviral, antitumor, antioxidative, insecticidal, hypoglycemic and wound repair. Although it has been reported in the literature that gallium has anti-inflammatory effects, it is still unclear ...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61K31/7028A61P29/00
CPCA61P29/00A61K31/192A61K31/7028A61K2300/00
Inventor 韩博杨文静王忠英刘永浩张艳马尚智藏洁郭刚周良学陈文张霄仪邹阳侯春宇田星赵娜
Owner SHIHEZI UNIVERSITY
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