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Evening primrose seed peptide as well as preparation method and application thereof

A technology of grass seeds and moongrowth, applied in the field of molecular biology, can solve the problems of low added value, waste discharge and environmental pollution, and achieve the effects of low production cost, good taste, and low bitter odor

Inactive Publication Date: 2019-01-01
吉林省全民健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of defatted evening primrose meal is used as feed and fertilizer or discarded, and the waste discharge pollutes the environment, and its added value is low

Method used

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  • Evening primrose seed peptide as well as preparation method and application thereof
  • Evening primrose seed peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Grind the degreased evening primrose seed meal without peculiar smell and mildew to 60 meshes, mix 80 parts of evening primrose seed protein powder with 20 parts of Lactobacillus acidophilus liquid and 20 parts of Streptococcus thermophilus liquid evenly, at this time the material The moisture content is controlled at 40%, and the mixed materials are piled up into a 40-ton fermentation heap. From the bottom of the fermentation heap to the top of the fermentation heap, each 10 cm interval is heated by a self-controlling electric heating cable. The heating temperature is 40 degrees; the surface of the fermentation pile is tightly covered with an oxygen barrier film, and the anaerobic fermentation is carried out for 3 days, and the fermentation is terminated when the small peptide content of the fermented product is greater than 70%; 0.2 parts of compound protease, 80 parts of drinking water, stirring at a pH value of 6 and a stirring speed of 120r / min, under continuous sti...

Embodiment 2

[0036]Grind the degreased evening primrose seed meal without peculiar smell and mildew to 50 meshes, mix 70 parts of evening primrose seed protein powder with 15 parts of Lactobacillus acidophilus liquid and 15 parts of Streptococcus thermophilus liquid evenly, and the material The water content is controlled at 30%, and the mixed materials are piled up into a 20-ton fermentation heap. From the bottom of the fermentation heap to the top of the fermentation heap, the self-controlling electric heating belt is heated on the plate at an interval of 15 cm. The heating temperature is 50 degrees; the surface of the fermentation pile is tightly covered with an oxygen barrier film, and the anaerobic fermentation is carried out for 5 days, and the fermentation is terminated when the small peptide content of the fermented product is greater than 70%; Mix 0.5 parts of compound protease, 85 parts of drinking water, stir under the conditions of pH value 7, stirring speed 150r / min, under cont...

Embodiment 3

[0038] Grind the degreased evening primrose seed meal without peculiar smell and mildew to 40 meshes, mix 60 parts of evening primrose seed protein powder with 10 parts of Lactobacillus acidophilus liquid and 10 parts of Streptococcus thermophilus liquid evenly, at this time the material The moisture content is controlled at 20%, and the mixed materials are piled up into a 1-ton fermentation heap. From the bottom of the fermentation heap to the top of the fermentation heap, the self-controlling electric heating belt is heated on the plate at an interval of 20 cm. The heating temperature is 60 degrees; the surface of the fermentation pile is tightly covered with an oxygen barrier film, and the anaerobic fermentation is carried out for 7 days, and the fermentation is terminated when the small peptide content of the fermented product is greater than 70%; 1 part of compound protease, 90 parts of drinking water, stirring at a pH value of 8 and a stirring speed of 180r / min, under con...

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Abstract

The invention provides evening primrose seed peptide as well as a preparation method and application thereof and belongs to the field of molecular biology. The preparation method takes degreased evening primrose seed meal as a raw material and comprises the following steps: firstly, carrying out microorganism solid fermentation to obtain evening primrose seed protein powder peptide with the size of 1500 to 8000 Daltons; then carrying out enzymolysis on evening primrose seed protein through proteinase until the size is 300 to 1000 Daltons; filtering through a cellulose membrane, and debitteringand deodorizing through an activated carbon adsorption column; after concentrating in vacuum, spraying and drying to obtain evening primrose seed peptide powder which can be directly absorbed by human bodies and has abundant nutrients. Compared with a pure enzymolysis method, the technology is low in cost and is more suitable for industrial production; the total protein content of the evening primrose seed peptide is greater than or equal to 90 percent and the content of low molecular weight peptide is greater than or equal to 80 percent; a product has small bitter odor and a good mouthfeel.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to an evening primrose seed peptide and its preparation method and application. Background technique [0002] Evening Primrose, commonly known as Sesame, also known as Evening Primrose, is a herb of the Willow family. Its seed oil has the effect of preventing blood lipid deposition and inhibiting platelet aggregation, and has been used in clinical medicine at present. In addition, the oil can also be used as a health supplement, an additive to nutritious food and a raw material for advanced cosmetics. Defatted evening primrose meal is a by-product of evening primrose seed oil extraction, which is rich in protein and amino acids. In Jilin Province alone, 3,000 tons of wild evening primrose seeds can be produced annually in Changbai Mountain, and 2,600 tons of cake residues will be piled up for uselessness. A certain oil factory in Harbin City produces more tha...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K2/00A23L33/18A23L2/39
CPCA23L2/39A23V2002/00A23L33/18C07K2/00C12P21/06A23V2200/14A23V2200/30A23V2200/324A23V2200/308A23V2250/55
Inventor 赵爽赵炳旭徐冰娜王春禹栾红迪
Owner 吉林省全民健食品有限公司
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