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A kind of biocontrol endophytic fungus and application thereof

An endophytic fungus and biocontrol technology, applied in the direction of application, fungi, biocides, etc., can solve the problems of unsatisfactory control measures, environmental pollution, etc., to effectively prevent and control Panax notoginseng root rot, reduce agricultural pollution, and promote The effect of growth

Active Publication Date: 2021-09-24
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide an efficient method for preventing and treating the root rot of Panax notoginseng in view of the serious occurrence of root rot of Panax notoginseng, and the area is expanding year by year. Biocontrol Bacteria for Seven Root Rot and Its Application

Method used

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  • A kind of biocontrol endophytic fungus and application thereof
  • A kind of biocontrol endophytic fungus and application thereof
  • A kind of biocontrol endophytic fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0018] The separation of embodiment 1 Panax notoginseng endophytic fungus

[0019] The specific process is as follows:

[0020] 1 Collection of samples

[0021] Healthy three-year-old Panax notoginseng plants were collected from the planting base in Miao Township, Wenshan County, Yunnan Province, packed into fresh-keeping bags and brought back to the laboratory for later use.

[0022] 2 Isolation of endophytic fungi

[0023] Plant cleaning procedure: Wash the soil on the surface of the plant under tap water, dry it slightly, cut the plant into small pieces, put it into a clean triangular bottle for ultrasonication, and wash it repeatedly until the water after cleaning becomes clear, turn it upside down, and empty the water.

[0024] Plant surface disinfection procedure: 0.1% Tween 20, 1min (soaked, treated with a beaker, the same plant uses a flask, the beaker requires sterilization) → the effective chlorine content is 5% sodium hypochlorite solution to treat the root sample...

Embodiment 2

[0027] Identification of Example 2 bacterial strain Z1-1031

[0028] 1 Observation of Morphological Characteristics

[0029] The strain Z1-1031 cultured for 7-10 days was removed from the plate and photographed against a black background. The colonies are gray-green with neat grayish-white edges. The surface is velvety hyphae, and yellow pigment is produced in the medium ( figure 1 ). A small amount of mycelium was taken out, made into a sheet, and the morphological characteristics of mycelium and spores were observed under an optical microscope. It was found that the hyphae had a transverse septum, and the conidiophores were branched many times to produce several rounds of asymmetrical stalks, shaped like brooms, and the conidia were spherical and smooth ( figure 2 ), according to the analysis of the above morphological characteristics, the strain Z1-1031 was initially identified as the Penicillium group.

[0030] 2 ITS sequence analysis

[0031] After the genomic DNA ...

Embodiment 3

[0032] Embodiment 3 Bacterial strain Z1-1031 fermentation and fermented liquid processing technology

[0033] The spore suspension of the above strains was 10 8 Cfu / ml is inoculated in PDB liquid medium, the inoculum volume is 1-2ml, 26-28°C, 120-150r / min, after 7-10 days of culture, the fermentation volume is 100mL / bottle. Use gauze to separate the bacterium balls from the fermentation broth, extract the fermentation broth three times with an equal volume of ethyl acetate, collect the extract and dry it under reduced pressure, and finally obtain a fermentation broth with a dry weight of 0.1-0.2 g / bottle. Use a small amount of methanol to dissolve the spin-dried product and transfer it out to obtain a crude extract of the fermentation broth.

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Abstract

The invention provides a notoginseng endophytic fungus for preventing and treating notoginseng root rot and its application, belonging to the technical field of crop disease prevention and control. The biocontrol strain is Penicillium sp. The strain inhibits the growth of pathogenic bacteria of notoginseng by producing active substances, and shows better control effect on root rot of notoginseng. When applied, it is used at the root irrigation stage of Panax notoginseng seedling or adult plant stage. The bacterial agent can also be mixed with organic fertilizer to make bacterial fertilizer. The biocontrol strain Penicillium No. Z1-1031 of the present invention is obtained from the root of Panax notoginseng, and its preservation number is CGMCC No.14134. The fungus blends harmoniously with soil ecology and Panax notoginseng plants, and is non-toxic and non-pathogenic, so it has very broad application prospects in the biological control of Panax notoginseng root rot.

Description

Technical field: [0001] The invention relates to a biocontrol endophytic fungus Penicillium sp. and its application, in particular to an endophytic fungus with probiotic effect on the growth of Panax notoginseng and its application in the prevention and treatment of Panax notoginseng root rot, belonging to Technical field of crop disease control. Background technique: [0002] Panax notoginseng is a perennial shade plant, and diseases frequently occur during the growth process, among which the root rot of notoginseng is the most important factor affecting the yield of notoginseng. At present, there is no mature and effective method for the prevention and treatment of root rot. The predecessors have done some research on the occurrence and prevention of the disease. Soil-borne pathogens are one of the main reasons for the root rot of Panax notoginseng. Fungal pathogens mainly include: Cylindrospora, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Verticillium, Phoma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/36A01P3/00C12R1/80
CPCA01N63/30C12N1/14C12N1/145C12R2001/80
Inventor 张怡轩章朦玥张天园谢君侯少阳
Owner SHENYANG PHARMA UNIVERSITY
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