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Preparation method for grape botrytis cinerea dry powder and using method thereof

A noble rot dry powder and technology of noble rot, which is applied in the field of preparation of grape noble rot dry powder, can solve the problems of noble rot wine products, limited output and difficult production, so as to promote the development of industrial technology and break the geographical limitations sexual effect

Active Publication Date: 2018-12-21
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the raw material of noble rot wine is not easy to produce, thereby causing the product and output of noble rot wine to be limited

Method used

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  • Preparation method for grape botrytis cinerea dry powder and using method thereof
  • Preparation method for grape botrytis cinerea dry powder and using method thereof
  • Preparation method for grape botrytis cinerea dry powder and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The steps of the preparation method of Botrytis cinerea dry powder of the present invention are: (1) activation culture of Botrytis cinerea; (2) liquid culture of Botrytis cinerea, (3) expanded cultivation of Botrytis cinerea; (4) Botrytis cinerea Bran culture; (5) Vacuum freeze-drying.

[0032] The steps of the using method of botrytis botrytis dry powder of the present invention are: (1) preparing emulsifier solution; (2) preparing noble rot powder solution; (3) separating botrytis dry powder clear liquid; (4) spraying and inoculating noble rot (5) Monitoring the maturity of noble rot grapes.

Embodiment

[0034] 1. the preparation method of noble rot dry powder (see figure 1 ):

[0035] (1) Activation culture of Botrytis cinerea: get preferred Botrytis cinerea strain and inoculate on PDA slant medium (20% potato, 2% glucose, 0.05% magnesium sulfate, 0.1% potassium dihydrogen phosphate, 2% agar), Cultivate in a constant temperature incubator at 25-28°C for 5 days;

[0036] (2) Liquid culture of Botrytis cinerea: pick 1 / 6 step (1) Botrytis cinerea after activation culture and inoculate into deep layer liquid culture medium (3% glucose, 4% potato juice, 0.1% potassium nitrate) together with PDA medium , 0.05% magnesium sulfate, 0.1% potassium dihydrogen phosphate), cultivated for 5 days at 180r / min, 25-28°C shaker culture conditions;

[0037] (3) Expanded cultivation of Botrytis cinerea: draw step 2) bacterial solution after liquid culture by 1% inoculum size, and inoculate into medium (3% glucose, 4% potato juice, 0.1% potassium nitrate, 0.05% sulfuric acid Magnesium, 0.1% pot...

experiment example

[0051] The present invention uses Botrytis C, a strain of Botrytis cinerea preferred in the early stage as material, uses a series of cultivation and preservation methods that are helpful to Botrytis cinerea, studies and compares two kinds of centrifugation and freeze-drying after liquid culture and freeze-drying after bran culture. method, the results show that after bran culture, vacuum freeze-drying is carried out without adding protective agent, the survival rate of Botrytis cinerea dry powder obtained is high, reaching 83%, and the activity of β-glucosidase is as high as 1.11U / mL.

[0052] 1. Purpose

[0053] Aiming at the problem that noble rot wine raw materials are difficult to form naturally, the dry powder preparation of Botrytis cinerea was developed to reduce the steps of strain selection, optimization, and expanded cultivation in the production of artificial noble rot wine. The dyeing situation is convenient for the generation of artificial noble rot grapes.

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Abstract

The invention aims to provide a preparation method for grape botrytis cinerea dry powder and a using method thereof. A whole process of the preparation method uses a series of technical measures helpful to accumulation and storage of spores of botrytis cinerea, mass propagation of the botrytis cinerea spores is guaranteed, and the final dry powder can keep original activity, the botrytis cinerea can be successfully generated by infecting while the dry powder is activated and sprayed to grapes. The preparation method for the grape botrytis cinerea dry powder comprises the following steps: (1) activated culture of the botrytis cinerea spores; (2) liquid culture of the botrytis cinerea spores; (3) amplification culture of the botrytis cinerea spores; (4) bran culture of the botrytis cinerea spores; and (5) freeze-drying in vacuum. The using method for the grape botrytis cinerea dry powder comprises the following steps: (1) preparing emulgator solution; (2) preparing botrytis cinerea dry powder solution; (3) separating botrytis cinerea dry powder clear liquid; (4) spraying and inoculating the botrytis cinerea; and (5) monitoring a maturity of the botrytis cinerea grape.

Description

1. Technical field: [0001] The invention relates to a preparation method and a use method of botrytis botrytis dry powder. 2. Background technology: [0002] Noble rot wine is a very precious sweet wine originating in Europe, which is produced by the infection of grape raw materials by a type of Botrytis cinerea. The natural conditions for the production of botrytized rot grapes are relatively harsh. It needs a unique micro-climate in the vineyard to produce it—cool and humid in the morning, and hot and dry in the afternoon. Therefore, the output of wine is small and the price is expensive, so it is called "noble rot wine". When grapes are infected with botrytis cinerea near the ripening stage, noble rot can occur, and noble rot grapes can be used as raw materials to brew noble rot wine. The process of botrytis rot involves complex enzymatic transformations and dehydration of grape berries, resulting in higher concentrations of sugars, acids, glycerol, minerals and aroma c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04A01G17/02A01G7/06C12R1/645
CPCA01G7/06A01G17/02C12N1/04C12N1/14
Inventor 陶永胜李爱华
Owner NORTHWEST A & F UNIV
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