A biocontrol composite bacterial agent las that can effectively prevent and control various crop wilt diseases

A technology of melon wilt and microbial composition, which is applied in the direction of plant growth regulators, plant growth regulators, biocides, etc., can solve the problems of poor colonization ability and unsatisfactory control effect of bio-control bacteria, and achieve the goal of saving costs Effect

Active Publication Date: 2018-05-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the biocontrol fungi of fusarium wilt mainly include Trichoderma and Gliocladium, but the control effect of the above-mentioned biocontrol fungi is not ideal, the main reason is that these biocontrol fungi have poor colonization ability in the host rhizosphere

Method used

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  • A biocontrol composite bacterial agent las that can effectively prevent and control various crop wilt diseases
  • A biocontrol composite bacterial agent las that can effectively prevent and control various crop wilt diseases
  • A biocontrol composite bacterial agent las that can effectively prevent and control various crop wilt diseases

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Source of strains: JC65 was isolated from cucumber rhizosphere soil; SM16 and CH01 were isolated from root soil of healthy tomato plants.

[0030] 2. Strain isolation method: using plate dilution method (refer to Microbiology Laboratory of Nanjing Institute of Soil Science, Chinese Academy of Sciences. Soil Microbial Research Method [M]. Beijing: Science Publishing Du, 1985.).

[0031] 3. Basis for strain screening: Determination of antagonism between watermelon wilt pathogen and biocontrol bacteria (confrontation culture method, Lin Fucheng, Li Debao. Bacillus subtilis (Bacillus subtilis) on phytopathogenic fungi Lysis effect[J].Plant Acta Pathologica Sinica, 2003, 33(2): 174~177. Greenhouse and field experiments on the effect of biocontrol bacteria on the control and growth promotion of watermelon Fusarium wilt.

[0032] 4. Strain identification method: identification by sequence sequencing of prokaryotic 16S rRNA coding gene fragments. The identification results ...

Embodiment 2

[0048] Inoculate the strains JC65, CH01 and SM16 on the LB plate and streak them respectively, and culture them in a biochemical incubator at 28°C for 14-16 hours. After a single colony grows, pick a single colony and insert it into a test tube containing 5 mL of LB culture medium. Cultivate in a shaker at 28°C and 200r / min to make a seed solution; inoculate the seed solution with 1% of the inoculum in a conical flask containing 500mL of LB culture solution, place at 28°C and 200r / min Cultivate in a shaker for 48 hours, and dilute the concentrations of the three bacterial solutions to 5×10 7 After CFU / mL, mix evenly according to the ratio of 1:1:1, which is the microbial complex agent.

Embodiment 3

[0049] Embodiment 3 greenhouse growth-promoting experiment

[0050] When the watermelon seedlings grow to the 3-4 leaf stage, select watermelon seedlings with consistent growth and transplant them into pots containing nutrient soil, and transplant 10 watermelon seedlings for each treatment. On the day after transplanting, 20mL of 5×10 7 CFU / ml of the corresponding biocontrol bacteria, 30 days after transplanting, add another biocontrol bacteria. Take clear water as a control. The growth promotion of each group was observed every day.

[0051] The results are shown in Table 4 and image 3 .

[0052] Total (average) fresh weight = underground fresh weight + aboveground fresh weight

[0053] Growth-promoting effect=[(average fresh weight of treatment-average fresh weight of clear water control) / average fresh weight of clear water control]×100%

[0054] Table 4 The growth-promoting effect of compound bacterial agent LAS on watermelon (transplanting 30d)

[0055]

[0056]...

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Abstract

The invention discloses a biocontrol multiple species inoculant LAS for efficiently controlling fusarium wilt of various crops. The biocontrol multiple species inoculant LAS is prepared from bacillus JC65, bacillus cereus CH01 and bacillus subtilis SM16. A mixed preparation is prepared by the three kinds of bacteria in accordance with a ratio of the numbers of viable organism of the bacteria 1 to 1 to 1, and the total concentration of viable organism in the mixture is 1 * 108 to 1010CFU / ml. The control effect of an LAS mixture on the crop fusarium wilt is 70% or more, and the growth promoting effect for a disease-free plant is up to 20%. The multiple species inoculant needs to be processed in sowing and transplanting.

Description

technical field [0001] The invention relates to a biocontrol composite microbial agent LAS for efficiently preventing and treating various crop wilts, and belongs to the technical field of agricultural biological control. Background technique [0002] Watermelons, cucumbers, melons and pumpkins play a pivotal role in the production of agricultural crops worldwide, and wilt disease is one of the main diseases that affect the yield and quality of the above four agricultural crops. Fusarium wilt is a devastating soil-borne disease caused by Fusarium oxysporum f.sp., commonly known as wilt disease, dead seedling disease, etc., and occurs worldwide. With the expansion of planting area and continuous cropping, the occurrence of Fusarium wilt has become more serious. [0003] At present, the main measures of fusarium wilt disease prevention and control still use chemical agents. Although chemical agents can control Fusarium wilt to a certain extent, at the same time, many benefic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/125C12R1/085
CPCA01N63/00A01N63/10C12N1/20A01N2300/00
Inventor 郭坚华蒋春号米丹丹郑颖
Owner NANJING AGRICULTURAL UNIVERSITY
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