A biocontrol strain and its application in preventing and controlling the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria

A technology of biocontrol strains and fritillary fritillaria, applied in application, pest control, plant growth regulator, etc., can solve the problem of lack of biocontrol bacteria for fritillary fritillaria soft rot, and achieve good effect of disease prevention and production increase , reduce planting costs, and control infestation

Active Publication Date: 2022-07-01
南京本源生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is a promising control method to control Fritillaria soft rot with biocontrol agents, but there is still a lack of biocontrol agents that can effectively control Fritillaria soft rot

Method used

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  • A biocontrol strain and its application in preventing and controlling the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria
  • A biocontrol strain and its application in preventing and controlling the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria
  • A biocontrol strain and its application in preventing and controlling the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1-Isolation, screening and identification of biocontrol strain Dm97

[0033] The present embodiment is the screening and identification process of the biocontrol strain Dm97 (Bacillus sp.), which specifically includes the steps:

[0034] (1) Screening of biocontrol strain Dm97

[0035] Source of strain: Dm97 was isolated from the root soil of Fritillaria fritillary, Nantong;

[0036] Strain isolation method: plate dilution method: sterilize 6 test tubes containing 9 mL of water, and press 10 to 10 6 Numbered in the order of the number, pipette to absorb 1 mL of cultured bacterial solution, pour it into a 10-fold diluted test tube, gently press the rubber on the pipette with your finger, blow and suck three times, and mix the bacterial solution and water well, from 10 times Pipette 1mL of diluent into the diluted test tube and inject 10 2 Repeat the mixing operation of the second step in the test tube with the doubling dilution. And so on until the dilution o...

Embodiment 2

[0043] Example 2-Cultivation of biocontrol strain Dm97 and preparation of its inoculum

[0044] This embodiment is a preferred method for culturing the biocontrol strain Dm97 obtained by screening in Example 1 and a method for preparing a bacterial agent thereof. The specific steps include:

[0045] (1) Cultivation of biocontrol strain Dm97

[0046] The biocontrol strain Dm97 was inoculated into LB medium (LB formula: sodium chloride 10g / L, tryptone 10g / L, yeast extract 5g / L, pH adjusted to 7.2). Scribe on the plate and cultivate in a biochemical incubator at 28°C for 14-16h. After a single colony grows, pick a single colony and put it into a test tube containing 5 mL of LB culture solution, and place it on a shaker at 28°C and 200 r / min. Cultivated for 25h in medium to make seed solution; inoculate the seed solution in a conical flask containing 500mL of LB culture solution with 1% of the inoculum, and place it in a shaker at 28°C and 200r / min for 48h. The prepared culture ...

Embodiment 3

[0049] Example 3-Application of microbial inoculum prepared by biocontrol strain Dm97

[0050] In this Example 3, the microbial inoculum prepared by the biocontrol strain Dm97 prepared in Example 2 was diluted with water to 5×10 7 Shake well after CFU / mL, and verify the disease prevention and control of Fritillaria fritillary. The specific steps include:

[0051] (1) Field disease prevention test (Zhejiang fritillary soft rot)

[0052] The field experiment was carried out in a professional cooperative of traditional Chinese medicine planting in Dongcheng, Hanyang Village, Haian County, Nantong City, Jiangsu Province, and the test plant was Fritillaria thunbergii Miq.

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Abstract

The invention relates to a biocontrol strain and its application in preventing and treating the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria fritillary. The above strain Dm97 was identified as Bacillus sp. The field test results show that the microbial inoculum made from the above strain Dm97 has a control effect of more than 90% on the soft rot of Fritillaria zeitensis, and the growth promotion effect on disease-free plants can reach more than 70%. The effect is above 50%. The above-mentioned microbial inoculants are used to prevent soft rot disease through soil treatment in the early stage, and can be used to better control soft rot disease through root treatment in the later stage. It has a good effect of disease prevention and yield increase, and has great potential to be developed into a commercial live product. Bacterial biocontrol agents.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicinal materials for disease prevention and treatment, in particular to a biocontrol strain and its application in preventing and treating the soft rot of Fritillary fritillary and promoting the growth of Fritillaria fritillary. Background technique [0002] Fritillaria fritillary is a traditional Chinese medicinal material of the genus Fritillaria. Fritillaria is used as a medicine with bulbs. It is a commonly used traditional Chinese medicine for both medicine and food. It has the effects of resolving phlegm and relieving cough, clearing heat and dispelling knots, and alkaloids are the main components of resolving phlegm and relieving cough. Zhe Fritillaria is suitable for growing in the shade of hills or under bamboo forests at lower altitudes. The main producing areas are in southern Jiangsu, northern Zhejiang and Hunan. [0003] Zhe Fritillaria is prone to major diseases such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P1/00A01P21/00C12R1/07
CPCC12N1/20C12N1/02A01N63/22
Inventor 陈刘军郑明子
Owner 南京本源生态农业科技有限公司
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