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Traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system and preparation method thereof

A technology of immune medicine and delivery system, applied in the field of medicine

Active Publication Date: 2018-12-21
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, small-sized (50nm) nanoparticles are difficult to penetrate into hypoxic regions, which is also a hot issue in the field of nano-preparation in recent years.

Method used

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  • Traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system and preparation method thereof
  • Traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system and preparation method thereof
  • Traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] This example takes ganoderma acid A-magnetic nanoparticles (G-SPIONs) as an example to illustrate the preparation method and characterization of traditional Chinese medicine-magnetic nanoparticles.

[0069] with FeCl 2 and FeCl 3 Mixed aqueous solution as raw material, pullulan as stabilizer, 3-aminopropyltriethoxysilane as amino source, precipitated by NaOH solution, NH 3 ·H 2 O adjust the pH and other steps to prepare SPIONs. Under magnetic stirring, the chloroform solution of ganoderma acid A was slowly dropped into the chloroform solution of SPIONs. After rotary evaporation, the aqueous solution was redissolved, and G-SPIONs were obtained after passing through the membrane. The G-SPIONs were observed by transmission electron microscopy to be round in shape and uniform in particle size ( figure 2 A); Conventional pharmaceutical analysis results show that when ganoderma acid A accounts for 6% of the mass fraction of iron oxide, its encapsulation efficiency is 82....

Embodiment 2

[0072] This example illustrates the preparation method of mannose-modified ganoderma acid A-magnetic nanoparticles (MG-SPIONs).

[0073] Disperse the G-SPIONs prepared in Example 1 in deionized water, slowly add equimolar amounts of succinic acid, 1.1eq of EDC and 1.3eq of NHS at room temperature, add equimolar amounts of mannose after stirring for 2 hours, and continue Stir at room temperature for 24 hours, then dialyze with running water to remove unreacted small molecules, and prepare MG-SPIONs.

[0074] With the same preparation and detection method, ganoderma acid A can also be replaced by ganoderma acid B, ganoderma acid, ganoderma triterpenes, ursolic acid, oleanolic acid, coroic acid, tripterine, ginseng triterpene saponins, Bupleurum Triterpene saponins, astragalus triterpene saponins, etc.

[0075] Succinic acid can also be substituted by malonic acid, glutaric acid, suberic acid, and the like.

Embodiment 3

[0077] This example takes Ganoderma acid A-magnetic nanoparticles (MG-SPIONC) as an example to illustrate the preparation method and characterization of traditional Chinese medicine-magnetic nanoclusters

[0078] The MG-SPIONs prepared in Example 2 were dispersed in 5% dimercaptosuccinic acid, stirred at room temperature for 24 hours, and the free cross-linking agent was removed by running water dialysis to prepare MG-SPIONC. The results of routine dialysis pharmaceutical analysis showed that the encapsulation rate of ganoderma acid A was 80.4±4.4%, and the drug loading capacity was 2.2±0.3%; the particle size of MG-SPIONC was 146.4±5.5nm, the PDI was 0.191±0.001, and the zeta potential was -14.45±1.64mV; MG-SPIONC was stored for 7 days and obvious settlement was observed.

[0079] With the same preparation and detection method, ganoderma acid A can also be replaced by ganoderma acid B, ganoderma acid, ganoderma triterpenes, ursolic acid, oleanolic acid, coroic acid, tripterin...

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Abstract

The invention relates to a traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system and a preparation method thereof. The traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system comprises superparamagnetic oxide nanoparticles, wherein the surface of each superparamagnetic oxide nanoparticle is anchored with a drug and a TAMs (tumor-associated macrophage) targeting ligand; the superparamagnetic oxide nanoparticles form a nanocluster structure through disulfide bonds; the nanocluster structure is externally packaged with protein. The traditional Chinese medicine-magnetic nanocluster chemical immunity drug delivery system has the advantages that the target finding advantages of magnetic trend, ligand modifying and reductive sensitization are integrated; the in-vivo circulation is stable, the tumor aggregation efficiency is high, and the TAMs targeting is precise.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a traditional Chinese medicine-magnetic nano-cluster chemoimmunomedicine delivery system and a preparation method thereof. Background technique [0002] Tumor tissue is not simply aggregates of tumor cells. According to the classic "seed-soil" theory of oncology, tumor cells are "seeds" and the tumor microenvironment is "soil", which plays a decisive role in the occurrence, development, and metastasis of tumors. The tumor microenvironment mainly includes some immune cells (such as tumor-associated macrophages (TAMs), T cells, dendritic cells, etc.), tumor-associated fibroblasts (TAFs), extracellular matrix, cytokines, and chemokines. Among them, TAMs play a central role in regulating the breast cancer immune network. TAMs are a diverse cell population, mainly divided into the classically activated M1 type and the alternatively activated M2 type. The former can activate tumor...

Claims

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Application Information

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IPC IPC(8): A61K47/54A61K47/69A61K47/02A61K9/51A61K31/575A61K41/00A61K45/00A61K47/42A61P35/00
CPCA61K9/5169A61K31/575A61K41/00A61K45/00A61K47/02A61K47/549A61K47/6939A61P35/00
Inventor 瞿鼎陈彦刘玉萍黄萌萌刘聪燕
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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