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Method for preparing high-quality indigo slurry based on lactic acid bacterium fermentation

A lactic acid bacteria fermentation, high-quality technology, applied in the field of indigo dyeing, can solve the problems of insufficient release of indoside, lack of dominance, long time consumption, etc., to achieve the effects of improving release rate, improving production quality and reducing production time.

Inactive Publication Date: 2018-12-18
陈岸瑛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The object of the present invention is to provide a kind of preparation method based on the high-quality blue indigo pulp of lactic acid bacteria fermentation, in order to solve the time-consuming in the prior art, the release of indigoside is not enough, lacks the dominant activating enzyme in the reaction system and The problem of low efficiency of indigo precipitation into indigo slurry

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  • Method for preparing high-quality indigo slurry based on lactic acid bacterium fermentation

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Embodiment 1

[0034] This embodiment provides a kind of preparation method based on the high-quality blue indigo pulp of lactic acid bacteria fermentation, such as figure 1 As shown, the method includes the following steps:

[0035] 1) Dilute the syrup into 120 catties of clear water at a mass ratio of 1:750;

[0036] 2) 2ml pre-cultured Bulgarian lactic acid bacteria were inoculated into the diluted medium; the number of colonies of the lactic acid bacteria was 10 8 CFU / mL.

[0037] 3) Sealed fermentation for 2 days, draw 1ml of the bacterial culture, dilute it 10 times, draw 200μl and spread it on the MRS-agar medium plate, cultivate it at 37°C for 16 hours, and calculate the number of viable bacteria in the bacterial culture to reach 1.6 ×10 8 CFU; Utilize the PH acidity meter (Starter 2C U.S. Ohaus imports PH acidity meter) to measure the pH of the bacterial liquid culture to 4.3;

[0038] Glycolysis of lactic acid creates a low pH environment and produces the biological enzyme glyc...

Embodiment 2

[0045] The present embodiment provides a kind of preparation method based on the high-quality blue indigo pulp of lactic acid bacteria fermentation, described method comprises the steps:

[0046] 1) Dilute the brown sugar into 160 jin of clear water with a mass ratio of 1:500;

[0047] 2) 2ml pre-cultured Bulgarian lactic acid bacteria were inoculated into the diluted medium; the number of colonies of the lactic acid bacteria was 10 7 CFU / mL.

[0048] 3) Sealed fermentation for 2 days, draw 1ml of the bacterial culture, perform doubling dilution, draw 200 μl and spread it on the MRS-agar medium plate, cultivate at 37°C for 16 hours, and calculate the number of viable bacteria in the bacterial culture to reach 1× 10 8 CFU uses a PH acidity meter (Starter 2C American Ohaus imported PH acidity meter) to measure the pH of the bacterial culture to 4;

[0049] 4) powdering the indigo; the mass ratio of the indigo to the fermentation broth is 1:14; mixing the pulverized indigo wit...

Embodiment 3

[0054]The present embodiment provides a kind of preparation method based on the high-quality blue indigo pulp of lactic acid bacteria fermentation, described method comprises the steps:

[0055] 1) Dilute the syrup into 80 catties of clear water at a mass ratio of 1:1000;

[0056] 2) 2ml pre-cultured Bulgarian lactic acid bacteria were inoculated into the diluted medium; the number of colonies of the lactic acid bacteria was 10 9 CFU / mL.

[0057] 3) Sealed fermentation for 2 days, draw 1ml of bacterial culture, perform doubling dilution, draw 200μl and spread it on the MRS-agar medium plate, incubate at 37°C for 16 hours, and calculate the number of viable bacteria in the bacterial culture to reach 2× 10 8 CFU; Utilize PH acidity meter (starter 2C U.S. Ohaus imports PH acidity meter) to measure the pH of bacterial liquid culture to 4.5;

[0058] 4) Pulverize the indigo; the mass ratio of the indigo to the fermentation broth is 1:10; mix the pulverized indigo with the fermen...

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Abstract

The invention discloses a method for preparing a high-quality indigo slurry based on lactic acid bacterium fermentation. The method comprises the following steps: 1, inoculating a sugar solution withlactic acid bacteria; 2, performing closed fermentation to obtain a fermentation solution having a pH value of 4-4.5; 3, immersing bluegrass in the fermentation solution obtained in step 2; 4, filtering out bluegrass residues in the fermentation solution, and reserving the fermentation solution; 5, adding calcium hydroxide, and performing uniform stirring until the pH value is 7.5-8.5; and 6, forming indigo insoluble in water in a fermentation system, standing the fermentation system until the indigo is completely precipitated, removing the obtained supernatant, and reserving the obtained indigo slurry to obtain the high-quality indigo slurry. The glycolysis metabolism way of the lactic acid bacteria is introduced to the preparation process of the indigo slurry, and the capability of lactic acid bacteria to generate lactic acid and glucoamylase through glycometabolism is used to actively regulate the formation process of the indigo slurry, so the production time is shortened, and the production quality is improved.

Description

technical field [0001] The invention relates to the technical field of indigo dyeing, in particular to a method for preparing high-quality indigo pulp based on lactic acid bacteria fermentation. Background technique [0002] Indigo dye is the most widely and frequently used vat dye in traditional plant dyeing technology, and it is widely used in the clothing dyeing and weaving industry. The completion of indigo dyeing technology requires two crucial technical links - the preparation of indigo pulp and the preparation of highly reactive dyeing water. [0003] In the traditional technological process, the preparation process of indigo pulp is generally as follows: first, the leaves, stems or roots of indigo are chopped, soaked in water for several days, and fermented to hydrolyze the glycoside bonds and release indol. Then add lime to rapidly oxidize indol into a blue precipitate under alkaline conditions, forming indigo pulp. [0004] The preparation process of this traditi...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 陈岸瑛赵兴旺
Owner 陈岸瑛
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