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Method for extracting ganoderma lucidum polysaccharide from ganoderma lucidum

A technology of Ganoderma lucidum polysaccharide and Ganoderma lucidum, applied in the field of biopharmaceuticals, can solve the problems of unsatisfactory extraction effect, difficult polysaccharide extraction, high degree of lignification, etc., and achieve the effect of reducing solvent consumption, short processing time and simple operation steps

Inactive Publication Date: 2018-12-18
GUANGXI XIUMEI ZHUANGXIANG ENERGY ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional hot water extraction method has the problems of long extraction time, high temperature and low yield
In order to overcome the shortcomings of the traditional water extraction method, in recent years, new extraction technologies and methods such as ultrasonic or microblog-assisted extraction, ultra-high pressure, and enzymatic method have emerged, but the extraction effect of these methods is not ideal.
Different from other edible fungi, the fruiting body of Ganoderma lucidum has a high degree of lignification and a complex structure. Generally, polysaccharides and lignin are bound together by physical or chemical bonds. It is difficult to extract polysaccharides by general methods.
In order to overcome the defects of the above methods, the invention patent with application number 201510764260.0 discloses a method for extracting Ganoderma lucidum polysaccharides from the fruiting bodies of Ganoderma lucidum, using sodium chlorite-glacial acetic acid oxidation system to digest lignin and fibers in the fruiting bodies of Ganoderma lucidum To release more polysaccharides from Ganoderma lucidum, thereby improving the extraction efficiency of polysaccharides from Ganoderma lucidum, but the extraction rate is still not high, and the quality of polysaccharides is affected by acid-base treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting ganoderma polysaccharide from ganoderma, comprising the following steps,

[0028] S1. Degreasing: dry and coarsely crush the fruiting body of Ganoderma lucidum; add 8 times the weight of ethanol, the volume fraction of the ethanol is 85%, reflux extraction at 75° C. for degreasing to obtain a crude extract. Ganoderma lucidum fruiting bodies are coarsely crushed to 50 meshes.

[0029] S2. Infiltration: the crude extract is dried and broken into 500 meshes. Then add 6 times the weight of water and soak at 60°C for 2 hours to obtain a pretreatment solution.

[0030] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 7, then add a complex enzyme of 5% of the mass of Ganoderma lucidum fruiting body, treat at 40-55° C. for 1-3 hours, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 90° C. for 15 minutes.

[0031] The compound enzyme comprises cellulase, hemicellulase, pectinase, xylanase, ɑ-...

Embodiment 2

[0036] A method for extracting ganoderma polysaccharide from ganoderma, comprising the following steps,

[0037] S1. Degreasing: dry and coarsely crush the fruiting body of Ganoderma lucidum; add 5 times the weight of ethanol, the volume fraction of the ethanol is 90%, reflux extraction at 80° C. for degreasing to obtain a crude extract. Ganoderma lucidum fruiting bodies are coarsely crushed into 40 meshes.

[0038] S2. Infiltration: the crude extract is dried and broken into 600 meshes. Then add 5 times the weight of water and soak at 70°C for 1 hour to obtain a pretreatment solution.

[0039] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 6, then add a compound enzyme of 3% of the mass of Ganoderma lucidum fruiting body, treat at 55° C. for 1 hour, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 85° C. for 20 minutes.

[0040] The compound enzyme comprises cellulase, hemicellulase, pectinase, xylanase, ɑ-amyl...

Embodiment 3

[0045] A method for extracting ganoderma polysaccharide from ganoderma, comprising the following steps,

[0046] S1. Degreasing: dry and coarsely crush the fruiting body of Ganoderma lucidum; add 10 times the weight of ethanol, the volume fraction of which is 80%, and extract under reflux at 75°C for degreasing to obtain a crude extract. Ganoderma lucidum fruiting bodies are coarsely crushed to 50 meshes.

[0047] S2. Infiltration: the crude extract is dried and broken into 400 meshes. Then add 5 times the weight of water and soak for 3 hours at 50°C to obtain a pretreatment solution.

[0048] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 8, then add a complex enzyme of 8% of the mass of Ganoderma lucidum fruiting body, treat at 40° C. for 3 hours, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 95° C. for 10 minutes.

[0049] The compound enzyme comprises cellulase, hemicellulase, pectinase, xylanase, ɑ-amyla...

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PUM

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Abstract

The invention provides a method for extracting ganoderma lucidum polysaccharide from ganoderma lucidum, and relates to the biopharmaceutical technical field. The extraction method includes degreasing,infiltration, enzymatic treatment, ultrasonic extraction, secondary extraction and alcohol precipitation. Through synergistic effect of degreasing, wall-breaking treatment, enzyme treatment and an ultrasound-assisted method, the extraction method has the advantages of simple operation steps, solvent saving, high product yield, short treatment time and the like.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for extracting ganoderma polysaccharides from ganoderma lucidum. Background technique [0002] Ganoderma lucidum is the dry fruiting body of the polyporaceae fungus Chizhi or Zizhi. Ganoderma lucidum is a precious medicinal fungus, which belongs to the genus Ganoderma lucidum of the Basidiomycetes Polyporaceae family. It has a medicinal history of more than 2,000 years in my country. Ganoderma lucidum was first recorded in "Shen Nong's Materia Medica". Physicians of all dynasties believed that Ganoderma lucidum can treat many diseases and is a precious medicine for nourishing, strengthening and strengthening the body. At present, it has been isolated from Ganoderma lucidum: triterpenoids, polysaccharides, nucleosides, sterols, alkaloids, furan derivatives, amino acid polypeptides, inorganic elements and fatty acids and other components. Among them, Ganoderma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 谢英健
Owner GUANGXI XIUMEI ZHUANGXIANG ENERGY ENVIRONMENTAL PROTECTION CO LTD
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