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Method for promoting accumulation of carotenoids and beta-carotene in Dunaliella by using beta-ionone

A carotene and ionone technology, which is applied in the field of food science, can solve the problems of limiting the output of beta-carotene, long cycle, etc., and achieves the effects of high yield, strong resistance to stress and increasing yield

Active Publication Date: 2018-12-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of cultivating Dunaliella is relatively long, which relatively limits the production of β-carotene. Finding substances that can promote the accumulation of β-carotene in Dunaliella has become a key technical problem in this field.

Method used

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  • Method for promoting accumulation of carotenoids and beta-carotene in Dunaliella by using beta-ionone
  • Method for promoting accumulation of carotenoids and beta-carotene in Dunaliella by using beta-ionone
  • Method for promoting accumulation of carotenoids and beta-carotene in Dunaliella by using beta-ionone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Activation culture

[0035] First prepare Dunaliella liquid culture medium, its composition is: NaNO 3 0.420g / L, NaCl 87.690g / L, NaH 2 PO 4 2H 2 O 0.015g / L, NaHCO 3 0.840g / L, KCl 0.074g / L, MgSO 4 ·7H 2 O 1.230g / L, CaCl 2 2H 2 O 0.044g / L, Fe-EDTA solution (Na 2 EDTA 1.804g / L, FeCl 3 ·6H 2 O 0.483g / L) 0.5mL / L, A5 trace element solution (H 3 BO 3 2.860g / L, MnCl 2 4H 2 O 1.810g / L, ZnSO 4 ·7H 2 O 0.220g / L, CuSO 4 ·5H 2 O 0.079g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 (0.039g / L) 1mL / L;

[0036] Then the cells of Dunaliella pasteurii were inoculated in the above-mentioned liquid medium, the light intensity was 8000Lx, the light-dark cycle was 14h:10h (14h light, 10h no light) (light-dark alternating culture), the temperature was 26°C, and the temperature was 26°C, with 50r / min speed vibration culture, to obtain synchronous growth of algae liquid.

[0037] (2) Induction culture

[0038] When the algal cells in the algal liquid are in the logarithmic growth...

Embodiment 2

[0049] (1) activation culture (with embodiment 1)

[0050] (2) Induction culture

[0051] Same as Example 1, except that the culture was continued for 6 hours after adding β-ionone.

[0052] (3) pigment extraction (with embodiment 1)

[0053] (4) Content determination

[0054] The method for determining the content of total carotenoids and β-carotene is the same as in Example 1, and it is found that adding 2 to 20 mg / L of β-ionone to treat Dunaliella pasteurii for 6 hours, the ratio of total carotenoids and β-carotene content The control group without β-ionone treatment increased by 9.3-33.0% and 16.2-38.1% respectively (see figure 2 ).

Embodiment 3

[0056] (1) activation culture (with embodiment 1)

[0057] (2) Induction culture

[0058] When Dunaliella pasteurii cells were in stationary phase (OD 630 =1.421), add 0, 1, 2, 5, 20 and 50mg / Lβ-ionone to the algae liquid respectively, and continue culturing for 4 days.

[0059] (3) pigment extraction (with embodiment 1)

[0060] (4) Content determination

[0061] After calculation, it was found that adding 1, 2 and 5 mg / L of β-ionone to treat Dunaliella pasteurii for 4 days, the content of total carotenoids and β-carotene increased by 13.9-13.9% compared with the control group without β-ionone. 28.6% and 31.0~39.3%, while the high concentration of 50mg / L β-ionone was treated for 4 days, the total carotenoid and β-carotene content was significantly higher than that of the control group due to the decrease in the cell biomass of Dunaliella pasteurii. reduce (see image 3 ). It shows that low concentration of β-ionone can promote the accumulation of total carotenoids and β...

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Abstract

The invention belongs to the technical field of food science, and discloses a method for promoting accumulation of carotenoids and beta-carotene in Dunaliella by using beta-ionone. The method comprises the following steps: carrying out induced culture on Dunaliella cells to be induced in a medium containing beta-ionone, accumulating beta-carotene in carotenoids and carotenoids in the algae cells,and then extracting a pigment. The Dunaliella cells to be induced are Dunaliella cells in a logarithmic phase and / or a stationary phase. The method of the invention is simple and feasible, is low-cost, and can significantly increase the yield of total carotenoids and beta-carotene; and Dunaliella is salt-tolerant single-celled green algae, requires simple culture conditions and is not easily contaminated by other microorganisms, which is beneficial to large-scale cultivation and meets the standards of industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of food science, and in particular relates to a method for promoting Dunaliella to accumulate carotenoids and beta-carotene by using beta-ionone. Background technique [0002] Carotenoids are a general term for a class of important natural pigments, which are widely distributed in higher plants, algae, and some photosynthetic and non-photosynthetic bacteria and fungi. Carotenoids have important physiological functions for animals and humans, such as improving vision, anti-oxidation, improving immune function and preventing cardiovascular diseases. Carotenoids are used industrially as nutritional supplements, cosmetics, and food coloring and coloring agents. Among the carotenoids, β-carotene is a natural orange-yellow pigment. It is rich in β-carotene in carrots, papayas, mangoes, and green vegetables. Natural β-carotene is the precursor of vitamin A, which is the vitamin A in human body. It also has anti-o...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 姜建国梁明华万林
Owner SOUTH CHINA UNIV OF TECH
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