Microbial agent of Bacillus velezensis
A technology of microbial inoculum and bacillus, applied in the directions of microorganisms, microorganisms, biocides, etc., to achieve the effects of large spore production, diverse formulations and convenient use methods
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[0043] Two, the preparation of the former powder of Bacillus Velez 1721
[0044] Using Bacillus Velez 1721 as an active ingredient can be used to make a variety of microbial preparations. To prepare the bacteria for the preparation of microbial inoculum, it is necessary to prepare the original powder of Bacillus Velez 1721. The preparation steps of the original powder are as follows:
[0045] A. Inoculate Bacillus Velez 1721 into LB liquid medium, shake culture to bacterial density OD 600 The value is 0.6-0.8, and the strain is obtained;
[0046] The content of tryptone in the LB liquid medium is 9-11g / L, the content of yeast extract is 4-6g / L, the content of sodium chloride is 9-11g / L, and the pH value of the LB medium is 7.0-7.2;
[0047] B. Insert the bacterial classification in step A into the seed tank by 9~11% of the medium volume used in the seed tank, the cultivation temperature of the seed tank is 35~37°C, the tank pressure is 0.05~0.07MPa, and the ventilation ratio ...
Embodiment 1
[0073] The preparation steps of Bacillus 1721 granules are as follows:
[0074] Prepare granules according to the above steps, and the specific composition content and process parameters are shown in the following table:
[0075] Table 2
[0076] project
Embodiment 2
[0078] The preparation steps of Bacillus Velez 1721 wettable powder are as follows:
[0079] Prepare the wettable powder according to the above steps, and the specific component content and process parameters are shown in the following table:
[0080] table 3
[0081]
[0082]
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