DCs vaccine based on phospholipid hybrid polymersome jointly encapsulating antigen and dual immunoagonists and preparation method and application thereof
An agonist and polymer technology, applied in the field of biomedical engineering, can solve the problems of incomplete maturity, lack of cross-presentation of DCs, inability of DCs to effectively carry antigens and activators, etc., to enhance immune response and maximize targeting effect , The effect of optimizing the immune effect
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[0035] The present invention provides a DCs vaccine based on phospholipid hybrid polymer vesicles co-encapsulating antigens and dual immune agonists. The preparation method comprises steps:
[0036] S1: Dissolve the amphiphilic triblock copolymer PCL-b-PEG-b-PCL and the immune stimulant in an organic solvent; Ultrasonic cell disruptor was used to sonicate for 5 minutes, and the antigen solution was dropped into the sonication process to obtain the primary emulsion;
[0037] Wherein, the mass ratio of the amphiphilic triblock copolymer PCL-b-PEG-b-PCL and the immune stimulant is 20mg:2mg; the molecular weight of the amphiphilic triblock copolymer PCL-b-PEG-b-PCL It is 10000-24000, preferably 16000, wherein the mass percentage of PEG hydrophilic segment is greater than 45%; the amphiphilic triblock copolymer PCL-b-PEG-b-PCL is preferably PCL 4000 -PEG 8000 -PCL 4000 The immune stimulant is one or more of TLR4, TLR7 / 8, TLR1, TLR2, TLR5, TLR6, TLR3 and TLR9; preferably, the TLR...
Embodiment 1
[0049] This example provides a DCs vaccine based on phospholipid hybrid polymer vesicles co-encapsulating antigens and dual immune agonists. The preparation method includes steps:
[0050] S1: 20 mg amphiphilic triblock copolymer PCL 4000 -PEG 8000 -PCL 4000 and 2mg of TLR7 / 8 agonist IMQ were dissolved in 1mL of dichloromethane; after fully dissolving, in an ice bath, ultrasonicated for 5min with a 3mm probe and an ultrasonic cell disruptor with power adjusted to 25% (16W). Add 200 μL of 10 mg / mL OVA antigen solution to obtain a primary emulsion.
[0051] S2: Under the condition of magnetic stirring at 200rpm, drop the primary emulsion into 10mL of swollen polyvinyl alcohol (PVA) solution with a mass fraction of 2%, and the dropping time is 2min, then wash it with 1mL deionized water. Immediately, the cleaned mixture was ultrasonicated for 10 min with a 5mm probe and an ultrasonic cell disruptor with power adjusted to 30% (22W) in an ice bath to obtain a secondary emulsion....
Embodiment 2- Embodiment 11
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