Use of interaction of Hippo-YAP channel and Wnt channel in adipose-derived stem cell cell-spectrum fate choice

Abstract

The invention discloses use of interaction of a Hippo-YAP channel and classical Wnt in preparing a product for affecting adipose-derived stem cell cell-spectrum fate choice, and specifically disclosesuse of mutual combination of a YAP protein and beta-catenin in preparing a drug for affecting adipose-derived stem cell cell-spectrum fate choice. The invention provides use of substances for inhibiting mutual combination of the YAP protein and the beta-catenin in preparing a product for promoting beta-catenin to transfer into cell nucleus, or use of substances for inhibiting mutual combination of the YAP protein and beta-catenin in preparing a product for improving TCF transcriptional activity or use of substances for inhibiting mutual combination of the YAP protein and beta-catenin in preparing a product for promoting the classical Wnt channel in the cell nucleus.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and specifically relates to the use of the interaction between a Hippo-YAP pathway and canonical Wnt in the preparation of products for affecting the fate selection of adipose stem cell cell spectrum, and more specifically relates to the mutual combination of YAP protein and β-catenin Use in the preparation of products for influencing the proliferation of adipose stem cells and the choice of cell lineage fate. Background technique [0002] With the rapid development of road traffic and mechanized production, fractures caused by high-energy trauma such as violence, such as comminuted fractures and nonunion after fractures, are the most difficult problems in orthopedics. In clinical practice, autologous bone or allogeneic bone is usually used Transplantation method to treat such diseases. However, autologous bone transplantation damages the site where the bone was taken and the amount of bone...

AI Technical Summary

Problems solved by technology

[0005] At present, there are mainly five methods for regulating the differentiation of adipose-derived stem cells: one is to select adipose-derived stem cells with strong osteogenic ability from the stem cell population by means of fluorescence-labeled flow cytometry and then use them for expansion. One-time use of adipose stem cells requires fluorescent labeling and flow cytometry screening, which is time-consuming and laborious, which reduces the advantages of adipose-derived stem cells and ease of use; the second is to use osteogenic induction fluid to induce osteogenesis of adipose-derived stem cells, and then apply them to tissues To promote the healing of bone defects on engineered bone, this method only exerts the osteogenic ability of adipose-derived stem cells in vitro in advance, and does not essentially promote the osteogenic differentiation of adipose-derived stem cells, and has no effect on the adipogenic differentiation of adipose-derived stem cells; The third is to use BMP and other cytokines that can promote the osteogenic differentiation of adipose-derived stem cells to directly add to the culture medium or play a role on adipose-derived stem cells in vivo in various slow-release ways, which can essentially improve the osteogenesis of adipose-derived stem cells differentiation ability, but its action time is limited, and it is difficult to maintain a stable and long-lasting effect of promoting osteogenesis or inhibiting adipogenesis; the fourth is to use small molecular compounds, such as dexamethasone and statins, to intervene in adipose-derived stem cells to improve their osteogenic ability. The first method is relatively simple and less costly, but the effect is limited; the fifth is to promote adipose osteogenic differentiation and inhibit adipogenic differentiation through genetic engineering, but the operation is cumbersome and expensive, and it is difficult to be widely promoted clinically.

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