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Method for improving dehydration performance of citric acid wastewater sludge by using penicillium oxalicum

A technology of citric acid wastewater and Penicillium oxalicum, which is applied in dehydration/drying/concentrated sludge treatment, biological sludge treatment, etc. It can solve the problems of biological conditioning that have not been reported, the source of the bacteria is not specified, and the cultivation period is long. Achieve the effect of short separation and purification cycle, easy operation, and improved dehydration performance

Active Publication Date: 2018-11-30
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing Chinese Patent Publication No. CN107176781A discloses a method for improving the dewatering performance of sludge by using the mycelium of yellow-blue fungus, but the limitation of the method is that the cultivation time of the mycelium needs 4 to 10 days. The cycle is long, and the source of the bacteria is not specified, which is not conducive to application
Moreover, most of these studies focus on municipal sludge, and there is no report on bioconditioning of food fermentation sludge with high organic matter such as citric acid sludge.

Method used

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  • Method for improving dehydration performance of citric acid wastewater sludge by using penicillium oxalicum
  • Method for improving dehydration performance of citric acid wastewater sludge by using penicillium oxalicum
  • Method for improving dehydration performance of citric acid wastewater sludge by using penicillium oxalicum

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Effect test

Embodiment 1

[0037] Example 1 Test sludge sample

[0038] The method of the present invention uses Penicillium oxalicum to improve the dewatering performance of citric acid sludge. During specific implementation, the test sludge sample is taken from the secondary sedimentation process of the oxidation ditch treatment process of a sewage treatment workshop of a citric acid production enterprise in Shandong The pond returns sludge. The company produces 60t of absolutely dry sludge every day. The sludge treatment method is to add PAM coagulation after concentration. The dosage is 6kg / t of absolute dry sludge. Finally, it is dehydrated by plate and frame filter press or belt filter press. The basic characteristics of the tested sludge are shown in Table 1.

[0039] Table 1 Basic characteristics of the tested sludge samples

[0040] Water content (%)

Embodiment 2

[0041] Example 2 Separation and purification of sludge filamentous fungi

[0042] Dilute the citric acid wastewater sludge sample by 10~10 5 After the different concentration gradients, they were inoculated into Sabouraud medium by the spreading plate method, and cultured in a constant temperature incubator at 25~30℃ for 2~3 days. Various fungal colonies were isolated and picked using the three-point inoculation method. The colony was expanded and cultivated in a petri dish. Repeat the inoculation 3 to 5 times to obtain fungi with different colony morphology after separation and purification. Fungi with different colony characteristics are respectively inoculated on the slant medium and stored in the refrigerator at 4°C.

Embodiment 3

[0043] Example 3 Screening and identification of filamentous fungi

[0044] The purified fungi were analyzed for morphology and growth characteristics, and the filamentous fungi with fast growth rate and developed mycelium were screened out, and then organic matter degradation experiments were performed on them, including gelatin liquefaction, cellulose hydrolysis, starch hydrolysis, and the characteristics were screened out. Obvious filamentous fungus, after 18SrDNA gene sequence and morphological identification, the morphology under microscope looks like figure 1 As shown, it was identified as Penicillium oxalicum.

[0045] (1) Gelatin liquefaction experiment

[0046] Medium configuration: 20 g of glucose, 5.0 g of peptone, 200 g of gelatin, dissolved in 1000 mL of distilled water for sterilization, and 10 mL of the sterilized medium solution was placed in a test tube. The fungus was inoculated on the culture medium and cultured at 25°C and 180 rpm for several days. The blank con...

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Abstract

The invention discloses a method for improving dehydration performance of citric acid sludge by using penicillium oxalicum and belongs to the field of treatment of wastewater sludge of citric acid industry. According to the method, the penicillium oxalicum with an extracellular polymer degradation function is separated, purified and screened out from the citric acid wastewater sludge, a fungal spore suspension with a concentration of 1.0*10<7> / mL to 1.0*10<8> / mL is prepared from the penicillium oxalicum, and then, a Martin liquid culture medium is inoculated with the fungal spore suspension for culture for 2 to 4 days, so that stable mycelia are formed. The mycelia are added into the citric acid wastewater sludge with a total suspended solid content of 1% to 4% according to a volume ratioof 5% to 20%, culture is carried out for 2 to 4 days after uniform mixing, the sludge specific resistance SRF and capillary water absorption time CST of the citric acid wastewater sludge can be separately lowered to (1-6)*10<13>m / kg and 6-12 seconds from (1-3)*10<14>m / kg and 25-43 seconds, and the dehydration performance is remarkably improved.

Description

Technical field [0001] The invention relates to the field of wastewater sludge treatment and disposal in the citric acid industry, in particular to a method for improving the dewatering performance of citric acid wastewater sludge by using Penicillium oxalate. Background technique [0002] Citric acid is mainly fermented, extracted and refined from starches such as corn and potato dried. The process will produce a large amount of high-concentration, difficult-to-degrade organic wastewater. Therefore, the residual sludge produced has a higher level than municipal sludge. The protein content of the sludge is extremely high, and the dewaterability is very poor. The moisture content of this type of sludge is still as high as 85% to 90% after dehydration. Because the higher water content brings great difficulties to its subsequent treatment and transportation, it is necessary to pre-treat the sludge before dewatering to improve its dewatering performance. [0003] At present, there are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02C02F11/12
CPCC02F11/02C02F11/12
Inventor 董黎明李影影汪苹郑心愿李杨杨
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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