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Complex enzyme preparation, preparation method thereof and method for producing biogas

A compound enzyme preparation and biogas technology, applied to biochemical equipment and methods, enzymes, lyases, etc., can solve problems such as low efficiency, insufficient reaction, and unsatisfactory effects, so as to increase biogas production and prolong the growth period of bacteria , The effect of microbial growth and metabolism on high-efficiency biogas fermentation

Pending Publication Date: 2022-03-18
RURAL ENERGY & ENVIRONMENTAL PROTECTION INST OF HEILONGJIANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are very few enzyme preparations available on the market for large-scale biogas production, and most of them have problems of low efficiency and insufficient reaction in the first stage of fermentation, and the effect is not particularly ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Compound enzyme preparation, including the following raw materials: 25kg of amylase, 25kg of cellulase, 20kg of xylanase, 8kg of lipase and 8kg of pectinase;

[0045] Among them, amylase is a mixture of α-amylase, β-amylase, glucoamylase and starch-1,6-glucosidase mixed with equal mass, cellulase is exo-β-glucanase, A mixture of endo-β-glucanase and β-glucosidase with equal mass, xylanase is β-1,4-endoxylanase, β-xylosidase, α-L - a mixture of arabinosidase, α-D-glucuronidase, acetylxylanase and phenolic esterase in equal quantities, lipase is phosphatase, sterolase and carboxylesterase in The mixture after equal mass mixing, pectinase is the mixture after equal mass mixing of polymethylgalactose, aldolase, polygalacturonase and polygalacturonate lyase;

[0046] The preparation method of compound enzyme preparation specifically comprises the following steps:

[0047] (1) take each raw material by the weight of above-mentioned composite enzyme preparation;

[0048] (2) ...

Embodiment 2

[0058] Compound enzyme preparation, including the following raw materials: 20kg of amylase, 30kg of cellulase, 15kg of xylanase, 10kg of lipase and 10kg of pectinase;

[0059] Among them, amylase is a mixture of α-amylase, β-amylase, glucoamylase and starch-1,6-glucosidase mixed with equal mass, cellulase is exo-β-glucanase, A mixture of endo-β-glucanase and β-glucosidase with equal mass, xylanase is β-1,4-endoxylanase, β-xylosidase, α-L - a mixture of arabinosidase, α-D-glucuronidase, acetylxylanase and phenolic esterase in equal quantities, lipase is phosphatase, sterolase and carboxylesterase in The mixture after equal mass mixing, pectinase is the mixture after equal mass mixing of polymethylgalactose, aldolase, polygalacturonase and polygalacturonate lyase;

[0060] The preparation method of compound enzyme preparation specifically comprises the following steps:

[0061] (1) take each raw material by the weight of above-mentioned composite enzyme preparation;

[0062] ...

Embodiment 3

[0072] Compound enzyme preparation, including the following raw materials: 30kg of amylase, 20kg of cellulase, 15kg of xylanase, 10kg of lipase and 10kg of pectinase;

[0073] Among them, amylase is a mixture of α-amylase, β-amylase, glucoamylase and starch-1,6-glucosidase mixed with equal mass, cellulase is exo-β-glucanase, A mixture of endo-β-glucanase and β-glucosidase with equal mass, xylanase is β-1,4-endoxylanase, β-xylosidase, α-L - a mixture of arabinosidase, α-D-glucuronidase, acetylxylanase and phenolic esterase in equal quantities, lipase is phosphatase, sterolase and carboxylesterase in The mixture after equal mass mixing, pectinase is the mixture after equal mass mixing of polymethylgalactose, aldolase, polygalacturonase and polygalacturonate lyase;

[0074] The preparation method of compound enzyme preparation specifically comprises the following steps:

[0075] (1) take each raw material by the weight of above-mentioned composite enzyme preparation;

[0076] ...

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PUM

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Abstract

The invention discloses a complex enzyme preparation which comprises 20-30 parts of amylase, 20-30 parts of cellulase, 15-25 parts of xylanase, 5-10 parts of lipase and 5-10 parts of pectinase, the amylase is at least one of alpha-amylase, beta-amylase, glucamylase and starch-1, 6-glucosidase, and the cellulase is at least one of alpha-amylase, beta-amylase, glucamylase and starch-1, 6-glucosidase. The preparation method of the compound enzyme preparation comprises the following steps: weighing the raw materials and stirring; the method for producing the biogas comprises the following steps: treating the livestock and poultry manure, the crop straws, the kitchen wastes and the vegetable wastes, uniformly stirring and mixing, adding the water, the compound enzyme preparation and the ferrous nitrate, and performing anaerobic fermentation, thereby obtaining the biogas. From the perspective of biogas anaerobic fermentation microbial metabolism and enzyme activity change rules, the compound enzyme preparation formula is used, so that the effects of promoting rapid and efficient hydrolysis of fermentation raw materials and growth and metabolism of biogas fermentation microorganisms are achieved, and the purpose of efficient and stable operation of a biogas production system through anaerobic fermentation of the fermentation raw materials is achieved.

Description

technical field [0001] The invention relates to the technical field of renewable energy utilization and environmental protection, and more specifically relates to a compound enzyme preparation, a preparation method thereof and a method for generating biogas. Background technique [0002] With the long-term use of coal, oil, and natural gas, these non-renewable fossil energy sources are facing the danger of depletion. At the same time, my country's agricultural production produces a large amount of waste such as crop stalks, roots, and stems every year, a large amount of animal manure produced by animal husbandry, and a large amount of organic waste in human production and life. [0003] Biogas technology, as a technology to produce flammable gases such as methane from livestock manure, crop stalks, food processing waste and wastewater, and alcohol waste, is getting more and more attention. The use of anaerobic fermentation of organic matter to produce biogas is an effective...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/26C12N9/42C12N9/24C12N9/20C12N9/18C12N9/88C12N9/34C12N9/16C12P5/02C02F11/04
CPCC12N9/2411C12N9/2437C12N9/248C12N9/20C12N9/88C12N9/2408C12N9/18C12N9/2414C12N9/2425C12N9/2428C12N9/2445C12N9/2482C12N9/2485C12N9/16C12P5/023C02F11/04C12Y302/01008C12Y302/01032C12Y302/01021C12Y301/01003C12Y301/01011C12Y302/01015C12Y402/02002C12Y302/01002C12Y302/01003C12Y302/01001C12Y302/01004C12Y302/01091C12Y302/01139C12Y301/03C12Y301/01001
Inventor 裴占江史风梅刘杰王粟李鹏飞于秋月周冰南
Owner RURAL ENERGY & ENVIRONMENTAL PROTECTION INST OF HEILONGJIANG ACAD OF AGRI SCI
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