Seaborne green method for safely preserving aquatic products
A technology for aquatic products and cold storage, which is applied in the field of green and safe preservation of aquatic products in non-ammonia fluoride evaporative cold rooms on ships, can solve the problems of high cost, inapplicability, and poor fresh-keeping quality.
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Embodiment 1
[0067] Example 1: Preparation of Antarctic Krill Protein Hydrolysate
[0068] Using 100kg Antarctic krill as raw material, first use papain (Nanning Pangbo Bioengineering Co., Ltd., enzyme activity 1 million U / g) for enzymatic hydrolysis, and the amount of enzyme added is 1000 U / g protein, at 40°C, pH 6.5 Enzymatic hydrolysis under conditions for 3 hours to obtain crude Antarctic krill enzyme solution; then the crude Antarctic krill enzyme solution was passed through an ultrafiltration membrane with a pore molecular weight of 100KDa and a membrane pore molecular weight of 50KDa to obtain a protein hydrolysate with an average molecular weight of 50-100KDa.
Embodiment 2
[0070] An antifreeze liquid, the components are as follows in mass percentage: 95% edible alcohol 15%, propylene glycol 10%, glycerol 2%, sodium chloride 3%, calcium chloride 1%, glycine 0.1%, Antarctic krill The protein hydrolyzate is 0.014%, the phospholipid is 0.1%, and the balance is water. The preparation method is as follows: first dissolve 300g sodium chloride, 100g calcium chloride, 10g glycine and 10g phospholipids in 6878.6g water, then add 1500g edible alcohol, 1000g propylene glycol, 200g glycerol and 1.4g Antarctic krill protein hydrolysate and stir well. Obtain antifreeze. The obtained antifreeze liquid has a freezing point of -25°C, good fluidity, and a viscosity of 46.8mPa·S, which is lower in viscosity and less volatile than the ternary refrigerant invented by Zhu Zhiwei and others [Chinese Patent 201210558113.4]. The antifreeze liquid will not freeze and crystallize during rapid stirring at low temperature.
[0071] The test found (freezing curve such as imag...
Embodiment 3
[0073] An antifreeze liquid, the components are as follows in mass percentage: 95% edible alcohol 20%, propylene glycol 15%, glycerol 4%, sodium chloride 4%, calcium chloride 2%, alanine 0.12%, Antarctica Krill protein hydrolysate is 0.30%, Tween (20) is 0.15%, and the balance is water. The preparation method is to first dissolve 400g sodium chloride, 200g calcium chloride, 12g alanine and 15g Tween (20) with 5443g water, then add 2000g edible alcohol, 1500g propylene glycol, 400g glycerol and 30g Antarctic krill protein to hydrolyze After stirring evenly, the antifreeze liquid is obtained. The obtained antifreeze liquid has a freezing point of -35°C, good fluidity at low temperatures, and a viscosity of 185mPa·S. Compared with Qin Haijie [Chinese Patent 201110174042.3], the quaternary refrigerant antifreeze liquid invented by Qin Haijie has less volatilization and lower salt content, and has less corrosion to equipment. The antifreeze liquid will not freeze and crystallize d...
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