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Detection method and detection device for Raman spectrum encoded liquid phase biochip

A technology of biochip and Raman spectroscopy, which is applied in the field of detection methods and devices of liquid-phase biochips, can solve the problems of poor decoding accuracy and stability of liquid-phase biochips, and achieve the elimination of poor decoding accuracy and stability and good application The effect of high foreground and peak stability

Inactive Publication Date: 2018-11-27
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to provide a detection method and device for a Raman spectrum-encoded liquid-phase biochip, thereby overcoming the problem of poor decoding accuracy and stability of the liquid-phase biochip for fluorescently encoded microspheres

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  • Detection method and detection device for Raman spectrum encoded liquid phase biochip
  • Detection method and detection device for Raman spectrum encoded liquid phase biochip
  • Detection method and detection device for Raman spectrum encoded liquid phase biochip

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Embodiment Construction

[0032] The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0033] Unless expressly stated otherwise, throughout the specification and claims, the term "comprise" or variations thereof such as "includes" or "includes" and the like will be understood to include the stated elements or constituents, and not Other elements or other components are not excluded.

[0034] Figure 1 to Figure 3 A schematic diagram showing a detection method of a Raman spectrum-encoded liquid-phase biochip according to a preferred embodiment of the present invention.

[0035] image 3 As shown, the preparation of the liquid-phase biochip encoded by Raman spectrum includes the following steps:

[0036] 1. Preparation of microchips. The micro-chip is made of transparent solid non-toxic material with a...

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Abstract

The invention discloses a detection method and a detection device for a Raman spectrum encoded liquid phase biochip. The detection method comprises the following steps: after a molecular reaction between a vector and a fluorescent probe, connecting the vector to nano metal particles to obtain a surface enhanced Raman vector with a Raman enhancing effect; reacting with a mixture of Raman to-be-detected molecules to prepare the Raman spectrum encoded liquid phase biochip; quantitively analyzing the liquid phase biochip through Raman spectrum to obtain a detection result of Raman to-be-detected molecules types; and quantitatively analyzing the liquid phase biochip through fluorescence microscopic imaging to obtain a detection result of quantity of the Raman to-be-detected molecules. The detection method has the advantages of an existing detection method of a fluorescent encoded microsphere-based liquid phase biochip, solves the problem that the existing liquid phase biochip is poor in encoding accuracy and stability fundamentally and has a very good application prospect in the fields of biomolecular analysis, clinical disease diagnosis and the like.

Description

technical field [0001] The invention relates to the field of molecular detection, and mainly relates to a liquid-phase biochip detection method and device. Background technique [0002] Liquid-phase biochip technology is a new biomolecular high-throughput technology that integrates fluorescence-encoded microspheres, flow cytometry, laser detection, and high-speed digital signal processing. Microspheres are used as carriers for hybridization reactions and signal detection. Usually, multi-color flow cytometry is used as a decoding and detection platform. By combining chip technology and flow cytometry technology, nucleic acid, protein detection of various biomolecules. Liquid-phase chip technology solves the problems of slow reaction rate, poor repeatability and flexibility of solid-state microarray chips. Compared with other traditional immunoassay methods, liquid-phase biochip technology has high throughput, multi-index joint detection, and high sensitivity. , high specifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 何永红谢鲁源陈雪静王蓓
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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