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Cosmetic composition for skin wrinkle repair and product thereof

A cosmetic composition and a technology for the composition are applied in cosmetic preparations, cosmetics, preparations for skin care, etc., and can solve problems such as lack of modification, poor histocompatibility, and skin allergies.

Inactive Publication Date: 2018-11-23
宁波美奈生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since these cytokines are expressed in lower organisms such as Escherichia coli or yeast, they lack post-translational modification of protein transcription in mammalian cells, including processes such as glycosylation, methylation, and terminal modification. Biological activity is very limited
Even if the cell growth factor fermented and prepared in mammals, it is still poor in histocompatibility when applied to humans, so that the skin causes allergies

Method used

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  • Cosmetic composition for skin wrinkle repair and product thereof
  • Cosmetic composition for skin wrinkle repair and product thereof
  • Cosmetic composition for skin wrinkle repair and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 Culture of human placental pluripotent stem cells

[0079] Collect discarded human placenta, cut into 1mm pieces 3 Size in 10ml Mesen Gro serum-free medium (containing 1 times serum-free supplement, 50μg / ml Dendrobium officinale polysaccharide, 10μg / m ginsenoside Rh1, 50μg / ml shikonin and 20μg / ml ascorbic acid), 37 ℃, 5 %CO 2 Placental pluripotent stem cells were obtained by primary culture in an incubator, and then subcultured.

[0080] Digested with 0.25% trypsin (containing 0.02% EDTA), from the first passage 25ml 175cm 2 At the beginning of the culture flask proliferation culture, pass to the second generation in one three-layer cell culture flask, then culture in the third generation in two five-layer cell culture flasks, and then in the fourth generation in six five-layer cell culture flasks , and then cultured in 18 five-layer cell culture flasks of the fifth generation, and then enlarged to culture in 42 five-layer cell culture flasks of the sixth g...

Embodiment 2

[0082] Example 2 Preparation of cosmetic raw materials derived from human placenta pluripotent stem cells

[0083] The human placental pluripotent stem cell culture solution was added to a dialysis bag with a molecular weight of 500 (Spectrum, USA), and placed in a refrigerator for dialysis and concentration, and the volume was concentrated by 5 times.

[0084] In addition, the culture solution of human placental pluripotent stem cells can also be concentrated by ultrafiltration using a 500-molecular-weight cellulose acetate membrane (Spectrum Corporation, USA), and the volume is concentrated 5 times.

Embodiment 3

[0085] Example 3 Detection of cytokine expression and secretion levels in human placental pluripotent stem cells

[0086] The human placental pluripotent stem cell culture fluid and its concentrate prepared in Example 1 were used to detect the secretion level of cytokines therein by an ELISA kit (R&D Systems, Inc., USA). The usage steps refer to the instruction manual.

[0087] The experimental groups are as follows: comparative experimental group A is to use no plant active extracts to cultivate human placental pluripotent stem cells, that is, Mesen Gro serum-free medium (containing 1 times serum-free supplement and 20 μg / ml ascorbic acid), to obtain culture medium; comparative experiment Group B is the culture of human placental pluripotent stem cells with Dendrobium officinale polysaccharide, that is, Mesen Gro serum-free medium (containing 1 times serum-free supplement, 110 μg / ml Dendrobium officinale polysaccharide and 20 μg / ml ascorbic acid), to obtain culture medium; com...

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Abstract

The invention provides a cosmetic composition for skin wrinkle repair. The composition is prepared by culturing human adult stem cells from tissues using dendrobium candidum polysaccharide, ginsenoside Rh1 and shikonin, and promoting protein synthesis and secretion of the adult stem cells; a stem cell culture solution obtained by the culture is rich in cell growth nutrition factor, has the effectof skin regeneration and repair, and becomes a biological beauty material for skin regeneration and repair; extracts of natural plants of dendrobium candidum and comfrey, oligopeptide 3 and carnosine,which have the effect of promoting the proliferation and growth of human epidermal cells and fibroblasts, can be added to directly prepare a regenerative repair essence, a mask, a cream cosmetic andthe like.

Description

technical field [0001] The invention relates to a cosmetic composition for wrinkle-removing and repairing skin and the product prepared therefrom. In particular, the present invention relates to using Dendrobium officinale polysaccharides, ginsenoside Rh1 and shikonin to cultivate human adult stem cells from tissues, to promote protein synthesis and secretion of adult stem cells, and to obtain stem cell culture fluid rich in cell growth nutrients The factor has the effect of skin regeneration and repair, and can be prepared into products such as regeneration and repair essence, mask, face cream and eye cream, which can be used for repairing skin wrinkles, scars and scars. Background technique [0002] At present, cosmetic raw materials are gradually abandoning chemicals as raw materials, advocating natural, pollution-free, and hormone-free raw materials to produce various cosmetics, such as active substances extracted from natural plants and active nutrients extracted from d...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/99A61K8/9794A61K8/9789A61Q19/08A61Q19/00A61P17/02
CPCA61P17/02A61Q19/00A61Q19/08A61K8/64A61K8/9789A61K8/9794A61K8/99A61K2800/805A61K2800/10
Inventor 不公告发明人
Owner 宁波美奈生物科技有限公司
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