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Cosmetic composition for resisting inflammation and removing acne of skin and product of cosmetic composition

A cosmetic composition and composition technology, applied in the direction of cosmetic preparations, cosmetics, dressing preparations, etc., can solve problems such as easy dependence on products, difficult diagnosis and treatment recovery, easy skin irritation, etc.

Active Publication Date: 2019-04-16
浙江奥比特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acne condition lasts for a long period, and it is difficult to diagnose and recover. Improper conditioning can easily aggravate the acne condition, causing skin sensitivity, dryness and peeling, redness, swelling, stinging pain, etc.
Many acne-removing products not only fail to improve and repair acne-prone skin, but also easily irritate the skin and cause recurrence of acne
Some anti-acne and anti-inflammatory products that have a great improvement effect on acne and acne can slightly improve the skin quality of acne, but if you use it too much or use it for a long time, you will easily rely on the product, and your skin will be dry and sensitive than before

Method used

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  • Cosmetic composition for resisting inflammation and removing acne of skin and product of cosmetic composition
  • Cosmetic composition for resisting inflammation and removing acne of skin and product of cosmetic composition
  • Cosmetic composition for resisting inflammation and removing acne of skin and product of cosmetic composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Culture of human peripheral blood T lymphocytes

[0083] Collect 200ml of human peripheral blood from healthy donors (with signed informed consent), separate and purify with lymphocyte separation medium to obtain 6.1×10 8 mononuclear cells in 150ml CTS TM Serum-free medium (containing 1 times serum-free supplement, 20 μg / ml Hedyotis diffusa extract, 10 μg / ml luteolin, 10 μg / ml paeoniflorin and 20ng / ml interleukin 10), 37°C, 5% CO 2 In the incubator, 50ml culture system, 75cm 2 Culture T lymphocytes in culture flasks.

[0084] When the T lymphocytes are cultured on the 3rd to 4th day, transfer to the cell culture bag, expand to 100ml for culture; after that, continue to expand and culture at intervals of 3 to 4 days, add 1 times the volume of fresh serum-free medium, and continue to culture until the first day On day 21, the culture fluid was collected and T lymphocytes were obtained; a total of 5.6 L of culture fluid was collected.

[0085] 4.48×10 10 M...

Embodiment 2

[0086] Example 2 Preparation of cosmetic raw materials derived from human T lymphocytes

[0087] Human T lymphocyte culture fluid was added to a 500 molecular weight dialysis bag (Spectrum, USA), placed in a refrigerator for dialysis concentration, and the volume was concentrated 5 times.

[0088] In addition, the culture solution of human T lymphocytes can also be concentrated by ultrafiltration using a 500-molecular-weight cellulose acetate membrane (Spectrum Corporation, USA), and the volume is concentrated by 5 times.

Embodiment 3

[0089] Example 3 Detection of cytokine expression and secretion levels in human T lymphocytes

[0090] The human T lymphocyte culture fluid and its concentrate prepared in Example 1 were used to detect the secretion level of cytokines therein by an ELISA kit (Ebioscience, USA), and the usage steps refer to the instruction manual.

[0091] The experimental groups are as follows:

[0092] Experimental group A: Human T lymphocytes were cultured using plant-free active extracts, namely CTS TM Serum-free medium (containing 1 times serum-free supplement and 20ng / ml interleukin-10) to obtain culture fluid;

[0093] Experimental group B: Human placental pluripotent T lymphocytes were cultured with Hedyotis diffusa extract, namely CTS TM Serum-free medium (containing 1 times serum-free supplement, 40 μg / ml Hedyotis diffusa extract and 20ng / ml interleukin-10) to obtain culture solution;

[0094] Experimental group C: Human placental pluripotent T lymphocytes were cultured with lu...

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PUM

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Abstract

The invention provides a cosmetic composition for resisting inflammation and removing acne of the skin. According to the composition, an extract of oldenlandia diffusa, galuteolin and paeoniflorin areextracted from tissue for cultivating human T lymphocytes, continuous multiplication of the T lymphocytes and secretion of cell factors are promoted, and a T lymphocyte cultivation solution obtainedthrough cultivation is rich in cell factor, has the functions of resisting inflammation and removing acne of the skin, becomes a raw biological beautifying material for resisting inflammation and removing acne of the skin; extracts of natural plants of oldenlandia diffusa, honeysuckles and paeonia lactiflora pallas can be added, and have the functions of inhibiting the inflammatory reaction of theskin, and essence, facial masks, cream-type cosmetics and other cosmetics which are capable of resisting inflammation and removing acne are directly prepared.

Description

technical field [0001] The invention relates to a cosmetic composition for anti-inflammatory and acne-removing skin and the product prepared therefrom. In particular, the present invention relates to the use of Hedyotis diffusa extract, luteolin and paeoniflorin to culture human T lymphocytes from tissues, to promote the proliferation and growth of T lymphocytes and the secretion of cytokines, and to cultivate T lymphocytes obtained by culturing It is rich in cell nutrient factors and has anti-inflammatory and anti-acne effects on the skin. It can be prepared into products such as anti-inflammatory and anti-acne essence, facial mask, face cream and eye cream. oxidation. Background technique [0002] In fact, acne, acne and acne marks mainly need to solve the problem of inflammation. Inflammation and acne can be said to be inseparable. Currently commonly used drugs, such as adapalene gel for external use, can improve acne marks and prevent the formation of acne pits; fusidi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/60A61K8/99A61K8/64A61Q19/00A61Q19/08A61Q19/02
CPCA61Q19/00A61Q19/005A61Q19/02A61Q19/08A61K8/602A61K8/64A61K8/9789A61K8/99A61K2800/522A61K2800/805A61K2800/592A61K2800/5922
Inventor 不公告发明人
Owner 浙江奥比特生物科技有限公司
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