Application of Pichia pastoris fermentation products expressing human lysozyme in broiler feed additives

A technology of human lysozyme and Pichia pastoris, which is applied in the fields of application, food processing, animal feed, etc., can solve the problems of limited sources, difficult sources of natural human lysozyme, and few reports on the scope and amount of application, so as to prevent mildew Mutation, prevention of animal infection, and the effect of reducing the chance of ingesting moldy feed

Active Publication Date: 2022-03-29
广州奇龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although human lysozyme has unique advantages and various pharmacological effects, and has many important clinical application values, it is extremely difficult to source natural human lysozyme
Human lysozyme is abundant in breast milk, but its source is extremely limited
In addition, although some research work on the application of lysozyme has been gradually carried out in the feed industry, the use of human lysozyme as an animal feed additive still needs further research and development, and there are few reports on its application range and application amount.

Method used

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  • Application of Pichia pastoris fermentation products expressing human lysozyme in broiler feed additives
  • Application of Pichia pastoris fermentation products expressing human lysozyme in broiler feed additives
  • Application of Pichia pastoris fermentation products expressing human lysozyme in broiler feed additives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of fermentation products of Pichia pastoris expressing human lysozyme

[0049] 1 Gene source:

[0050] The recombinant human lysozyme gene was synthesized by chemical synthesis, and then cloned into the pUC19 plasmid vector by DNA recombination technology. The cloned strain was confirmed to be the recombinant human lysozyme gene by nucleotide DNA sequence analysis.

[0051] 2 Preparation of recombinant human lysozyme gene:

[0052] 2.1 Recombinant human lysozyme gene design and synthesis of oligonucleotide primers by chemical synthesis:

[0053] P1: CCTCGAGAAAAGAGAGGCTGAAGCTAAGGTCTTTGAAAGGTG (SEQ ID NO: 3),

[0054] P2: GGAATTCTTACACTCCACAACCTTG (SEQ ID NO: 4).

[0055] 2.2 PCR amplification: RT product 10 μl, 10×PCR buffer 5 μl, 10 mmol / L dNTP 1 μl, 15 pmol / L P1 primer 1 μl, 15 pmol / L P2 primer 1 μl, Taq enzyme 1 μl (5u), redistilled water 31 μl, total volume 50 μl ;Reaction conditions: 94°C for 1 minute, 55°C for 1 minute, 72°C for 2 minutes, 35 cycles...

Embodiment 2

[0066] A broiler feed containing a fermented product of Pichia pastoris expressing human lysozyme, the composition of which is as follows:

[0067]

Embodiment 3

[0069] A broiler feed containing a fermented product of Pichia pastoris expressing human lysozyme, the composition of which is as follows:

[0070]

[0071]

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Abstract

The invention provides a broiler feed containing a fermented product of Pichia pastoris expressing human lysozyme, the percentage by weight of the fermented product of Pichia pastoris expressing human lysozyme is 0.1% to 10%, and the feed also includes the following parts by weight Composition: 50-60 parts of corn, 2-4 parts of corn oil, 20-30 parts of soybean meal, 2-4 parts of corn gluten powder (CP60%), 4-6 parts of DDGS, 2-4 parts of fiber meal, phosphoric acid 1-2 parts of hydrogen calcium, 1-2 parts of stone powder, 0.15-0.2 parts of salt, 0.5-1.5 parts of additive premix. The bacteriolytic activity of human lysozyme in this feed is 3 times higher than that of egg white lysozyme widely used at present. Non-antigenic and non-toxic, it can replace antibiotics as a safe and non-toxic animal feed additive.

Description

technical field [0001] The invention relates to the technical field of animal breeding feed, in particular to the application of a fermented product of Pichia pastoris expressing human lysozyme in animal feed additives. Background technique [0002] Feed additives are an important material prerequisite for the realization of feed industrial production and modern breeding. Without a modern feed additive industry, there will be no modernization of the feed industry and modern breeding. The main purpose of using antibiotic additives in animal feed is to provide an optimal environment for the small intestinal mucosa to facilitate the full absorption of nutrients, improve the utilization rate of nutrients, feed conversion rate and productivity, and at the same time prevent or treat animal infections and reduce animal health. mortality rate. Adding antibiotics to the diet has a significant effect on young animals in the growing period. Healthy piglets can gain weight even under p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/75A23K10/30A23K10/37A23K20/158A23K20/189A23K20/147A23K20/26A23K10/12
CPCA23K10/12A23K10/30A23K10/37A23K20/147A23K20/158A23K20/189A23K20/26A23K50/75Y02P60/87
Inventor 张华李亚男吕秋军夏枫耿陈达凯陈聪伟杨创钦郑金丽李月俏邓远倪陈禹兴梁佳潮
Owner 广州奇龙生物科技有限公司
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