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A kind of artificial biological dura mater and preparation method thereof

A dura mater and biological technology, applied in the field of artificial biological dura mater and its preparation, can solve the problems of poor suture performance and weak mechanical properties, and achieve the effects of improving clinical use safety, low cost, and preventing cerebrospinal fluid leakage

Active Publication Date: 2020-07-17
NKD PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this porous sponge-like membrane is limited in neurosurgery due to its weak mechanical properties and poor suturing performance during clinical use.

Method used

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  • A kind of artificial biological dura mater and preparation method thereof
  • A kind of artificial biological dura mater and preparation method thereof
  • A kind of artificial biological dura mater and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 A kind of artificial biological dura mater and preparation method thereof

[0053] Composition of artificial biological dura mater: The mass ratio of bacterial cellulose and collagen is 1:10, and the upper and lower surfaces of the bacterial cellulose membrane are evenly compounded with collagen by means of electrospinning. The bacterial cellulose membrane is produced by Acetobacter.

[0054] Artificial biological dura mater preparation method:

[0055] 1) Fermentation preparation of bacterial cellulose film: activate the cellulose-producing Acetobacterium to obtain activated strains, expand and cultivate the activated strains to obtain seed liquid, inoculate the seed liquid into the fermentation medium and ferment to obtain bacterial cellulose Fermentation broth, after 8 days of fermentation, a layer of bacterial cellulose film will be formed on the upper surface of the fermentation broth, which is taken out for use;

[0056] Wherein, the formula of t...

Embodiment 2

[0063] Embodiment 2 A kind of artificial biological dura mater and preparation method thereof

[0064] Composition of artificial biological dura mater: The mass ratio of bacterial cellulose and collagen is 1:10, and the upper and lower surfaces of the bacterial cellulose membrane are evenly compounded with collagen by means of electrospinning. The bacterial cellulose film is produced by microsarcinia.

[0065] Preparation:

[0066] 1) Fermentation preparation of bacterial cellulose film: activate the cellulose-producing Acetobacterium to obtain activated strains, expand and cultivate the activated strains to obtain seed liquid, inoculate the seed liquid into the fermentation medium and ferment to obtain bacterial cellulose Fermentation broth, after 8 days of fermentation, a layer of bacterial cellulose film will be formed on the upper surface of the fermentation broth, which is taken out for use;

[0067] Wherein, the formula of the fermentation medium is that every 500 m...

Embodiment 3

[0074] Embodiment 3 A kind of artificial biological dura mater and preparation method thereof

[0075] Composition of artificial biological dura mater: The mass ratio of bacterial cellulose and collagen is 0.5:10, and the upper and lower surfaces of the bacterial cellulose membrane are evenly compounded with collagen by means of electrospinning. The bacterial cellulose membrane is produced by Acetobacter.

[0076] Preparation:

[0077] 1) Fermentation preparation of bacterial cellulose film: activate the cellulose-producing Acetobacterium to obtain activated strains, expand and cultivate the activated strains to obtain seed liquid, inoculate the seed liquid into the fermentation medium and ferment to obtain bacterial cellulose Fermentation broth, after 8 days of fermentation, a layer of bacterial cellulose film will be formed on the upper surface of the fermentation broth, which is taken out for use;

[0078] Wherein, the formula of the fermentation medium is that every 5...

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Abstract

The invention belongs to the technical field of bio-based materials, and particularly relates to an artificial biological dura mater and a preparation method thereof. The artificial biological dura mater is prepared from the following raw materials of bacterial cellulose and collagen according to a mass ratio of (0.1 to 3):10; any crosslinking agent is not added, so that the adverse effect to therepair process of the dura mater by the crosslinking agent is reduced, and the clinical use safety of the dura mater product is improved. The artificial biological dura mater has the advantages that the artificial biological dura mater is prepared by an electrostatic spinning method; the pH (potential of hydrogen), temperature and other technology conditions are strictly controlled, so that the good biocompatibility and mechanical property are realized, the requirement of operation suture in the clinical use process is met, and the leakage of cerebrospinal fluid is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of bio-based materials, and in particular relates to an artificial biological dura mater and a preparation method thereof. Background technique [0002] The dura mater (dura mater) is a thick and tough double-membrane tissue between the skull and brain tissue, which constitutes an important natural protective medium for brain tissue, and trauma, inflammation, tumor erosion and surgical operations are all It can cause damage to the dura mater and destroy its integrity. Dural plastic surgery is an important operation step in the early and second phase repair of skull defects in craniomaxillofacial plastic surgery. It is indispensable for maintaining its anatomical integrity and protecting brain tissue. The repair materials for dural defects also directly affect the incidence of complications such as intracranial infection, epilepsy, encephalocele, and cerebrospinal fluid leakage after dural reconstruction, as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/20A61L27/24
CPCA61L27/20A61L27/24A61L27/50A61L2430/32C08L1/02
Inventor 石凌锋陶秀梅陈鹏尚丽霞
Owner NKD PHARMA CO LTD
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