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Plant immune resistant inducer and application thereof

An immune inducer, plant technology, applied in the direction of plant growth regulator, plant growth regulator, application, etc., can solve the problems that have not been reported in the research, and achieve the effect of good drug effect

Inactive Publication Date: 2018-11-16
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biocontrol Trichoderma has achieved good results in greenhouse and field tests, and a variety of commercial Trichoderma preparations have come out abroad, such as Topshield (Trichoderma harzianum T22) in the United States and Tirchodex (T. ), the domestic Trichoderma preparation has also passed the appraisal of the expert group in June 2002, and its biocontrol effect has also been affirmed, but there is no research report on the preparation of the biocontrol Trichoderma protein elicitor inducer. Trichoderma T2 fermentation broth protein elicitor (TraT2A) to prepare plant immune inducer to induce and enhance the disease resistance of lily

Method used

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  • Plant immune resistant inducer and application thereof
  • Plant immune resistant inducer and application thereof
  • Plant immune resistant inducer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of plant immune inducer formula and its quality evaluation

[0032] 1. Determination of the formulation of the inducer

[0033] A plant immune inducer formula is based on 1g of mass, and the distribution ratio of each component is:

[0034]

[0035] 2. Quality evaluation of inducers

[0036] After the above formula is determined, its pH value, foamability, heat storage and low temperature storage stability, etc. are measured, and the reference standards are as follows:

[0037] (1) Determination of low-temperature stability: refer to GB / T19137-2003 low-temperature stability determination method, keep the sample at 0°C for 1 hour, record whether there is solid or oily matter, continue to store at 0°C for 7 days, and perform centrifugation. Record the volume of solid precipitated.

[0038] (2) Heat storage stability: refer to GB / T 19136-2003 Determination method for heat storage stability of pesticides.

[0039] (3) Determination of pH value: me...

Embodiment 4 0

[0078] Example 4 20% TraT2A Inducer Induced Resistance to Schizophyllum in Lanzhou Lily and Detection of Enzyme Activity in Plants

[0079] 1. Test process

[0080] 1. The configuration of the spore suspension: take the Schizophyllum spores activated and cultured at 25°C for 7 days, add 10ml of sterilized water, scrape off the Schizophyllum spores on the culture dish with a sterilized applicator, and then put the sterilized The gauze is folded into 4 layers of filtration, the spore suspension after filtering is transferred to a 50ml beaker, and then the spore suspension is calculated with the hemocytometer counting method to calculate the concentration of the spore suspension, and then diluted to the required concentration (1 × 10 5 spores / mL).

[0081] 2. The surface of lily bulbs was wiped and disinfected with 75% alcohol, then disinfected with 0.1% mercuric chloride for 30 seconds, and then rinsed with sterilized distilled water for 3 to 5 times. They were divided into two...

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Abstract

The invention belongs to the technical field of biological pesticide, and particularly relates to a plant immune resistant inducer and application thereof. The plant immune resistant inducer is prepared by using a Trichoderma aureoviride T2 protein elicitor (TraT2A) which is adopted as a main effective active ingredient and a surfactant, a thickener, a stabilizer, an antifreeze and an antifoamingagent at a specific ratio. The prepared plant immune resistant inducer can be applied to induction of supply of enzyme activities related to disease resistance of lily, the disease resistance of lilycan be enhanced, and the preparation has no pollution to the environment and no harm to human beings and animals, and has good pesticide efficacy.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, in particular to a plant immune inducer and its application. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the fungus genus Deuteromycotina, Hyphomycetes, Hyphospora, myxospores, is a class of ubiquitous fungi, common in soil, is an important community of soil microorganisms, also found in In plant residues and animal feces, it can often be separated from plant root circles, leaf circles, seeds, and bulb surfaces. According to incomplete statistics, Trichoderma has antagonistic effects on at least 29 species of phytopathogenic fungi in 18 genera. Trichoderma aureoviride is a kind of Trichoderma with biocontrol function. The pathogenic bacteria have biocontrol function, and the Bacillus subtilis serine protease gene SS42 produced by it has biocontrol effects on five pathogens including Fusarium oxysporum, Sclerotinia sclerotiorum, Leaf blight, Poplar rot an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00A01P3/00
CPCA01N63/30
Inventor 梁巧兰张娜徐秉良魏列新吴琼李嘉明
Owner GANSU AGRI UNIV
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