Accurate and efficient eukaryotic biological gene identification method based on transcriptome

Abstract

The invention discloses an accurate and efficient eukaryotic biological gene identification method based on a transcriptome. The method comprises 1, quasi-identification gene expression prediction, 2,acquisition of a quasi-identification gene high expressed sample, RNA extraction and reverse transcription, 3, transcriptome sequencing, 4, transcriptome data analysis and storage, 5, transcriptome data retrieval and 6, gene identification primer design and gene identification. Through the close specie research report analysis, the transcriptome of the high expressed sample of the gene to be identified is determined, the target sequence of the gene to be identified is screened in the transcriptome, and the gene is identified and cloned based on the target sequence so that an accurate gene sequence is obtained. The method is suitable for identification of functional genes of most eukaryotes, is very accurate, satisfies the research demands of the functional gene identification in the research process, has a reasonable design, is easy to use, realizes a low cost and is suitable for promotion and use.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an accurate and efficient eukaryotic gene identification method based on transcriptome. Background technique [0002] Since the completion of the human genome, the genomes of more and more organisms have been sequenced. Genome-based identification of biological genes is relatively easy. However, the proportion of species whose genomes have been sequenced is very small, and most species need to identify their own genes through homologous cloning. Homologous cloning works better when the corresponding genes have been identified in closely related species. However, homologous cloning often fails to achieve satisfactory results in the identification of genes with low consistency among species and genes of some species with special evolutionary status. It is often necessary to analyze the conserved sites of the gene and design an incorporation primer, which may require several or even ...

AI Technical Summary

Problems solved by technology

Greatly increased the economic cost and time cost of identification