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Culturing method of Lonicera fragrantissima in vitro pollen germination

A technology of pollen germination and cultivation method, applied in the field of plant reproduction, can solve the problems that do not involve the pollen germination and cultivation technology of honeysuckle

Inactive Publication Date: 2018-11-06
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this patent does not relate to the germination and cultivation technology of honeysuckle pollen

Method used

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  • Culturing method of Lonicera fragrantissima in vitro pollen germination
  • Culturing method of Lonicera fragrantissima in vitro pollen germination
  • Culturing method of Lonicera fragrantissima in vitro pollen germination

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Embodiment 1

[0032] The present invention will be further described in detail by taking the honeysuckle in Xi'an Botanical Garden in Shaanxi Province as an example. The culture method for the germination of isolated pollen of Lonicera japonica described in this embodiment, the isolated pollen used is fresh pollen of Lonicera japonica.

[0033] In mid-March, in the Xi'an Botanical Garden Park, select the robust, disease-free and pest-free Eucalyptus honeysuckle plants, and pick the loose powdered flowers of the day from 9:00 to 10:00 in the morning, put them on sulfuric acid paper, and quickly bring them back to the laboratory. Lightly brush the pollen sacs that have loosened the pollen to collect the pollen.

[0034] (1) Sucrose concentration test

[0035] Prepare sucrose solutions with mass fractions of 15%, 20%, 25%, 30%, 35%, 40%, and 45% for later use.

[0036] Use a pipette to drop 1 drop of sucrose solution on the glass slide, stick the pollen lightly with a pin, put it in the sucr...

Embodiment 2

[0043] The present invention will be further described in detail by taking the honeysuckle in Xi'an Botanical Garden in Shaanxi Province as an example. The culture method for the germination of isolated pollen of Lonicera japonica described in this embodiment, the isolated pollen used is fresh pollen of Lonicera japonica.

[0044]In mid-March, in the Xi'an Botanical Garden Park, select the robust, disease-free and pest-free Eucalyptus honeysuckle plants, and pick the loose powdered flowers of the day from 9:00 to 10:00 in the morning, put them on sulfuric acid paper, and quickly bring them back to the laboratory. Lightly brush the pollen sacs that have loosened the pollen to collect the pollen.

[0045] Prepare 30% sucrose + boric acid solutions with concentrations of 100, 200, and 300 mg / L and 35% sucrose + boric acid solutions with concentrations of 100, 200, and 300 mg / L respectively. The sucrose solution was used as the control, and the ratio of the culture solution suita...

Embodiment 3

[0052] On March 15th, in the Xi'an Botanical Garden Park, the robust growth and no pests and diseases of Lonicera japonica plants were selected, and the loose powdered flowers of the day were picked from 9:00 to 10:00 in the morning, placed on sulfuric acid paper, and quickly brought back to the laboratory. Brush the pollen sacs that have loosened powder lightly, collect pollen, dry in the shade indoors for 48 hours, and store at -20°C, room temperature, and 5°C, respectively.

[0053] On March 22, March 28, April 7, April 17 and April 26, culture solutions with a mass fraction of 30% sucrose + 100 mg / L boric acid were prepared.

[0054] Use a pipette to drop 1 drop of culture solution on the glass slide, stick the pollen lightly with a pin, put it in the culture solution quickly and shake gently to make the pollen disperse, place it in an incubator for 3 hours at 12°C, and observe Pollen germination. When counting, the pollen tube length exceeds the pollen grain diameter as ...

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Abstract

The invention belongs to the technical field of plant sexual reproduction, and discloses a culturing method of Lonicera fragrantissima in vitro pollen germination. The culturing method comprises following steps: collected Lonicera fragrantissima pollens are dispersed in a culture solution, and culturing is carried out for about 3h in an incubator, wherein culturing temperature is controlled to be9 to 18 DEG C, preferably 12 DEG C, the culture solution comprises cane sugar at a mass concentration ranging from 15 to 45%, and 100 to 300mg / L of a boric acid solution, preferably cane sugar at a mass concentration of 30%, and +100mg / L of the boric acid solution. The Lonicera fragrantissima fesh pollen germination rate is capable of reaching 96.28%. The culturing method is suitable to be used for Lonicera fragrantissima in vitro pollen vitality measuring; operation is simple and convenient; the repeatability is high; the data is accurate and reliable; and foundation is provided for Lonicerafragrantissima pollen in vitro preservation and variety improvement.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a method for cultivating and germination of plant pollen, in particular to a method for cultivating the germination of isolated pollen of Lonicera japonica. Background technique [0002] Tulip Honeysuckle ( Lonicera fragrantissima Lindl. et Paxt.), also known as honeysuckle, goat milk, and xiangjilizi, is a semi-evergreen shrub of the genus Lonicera in the Lonicera family. It flowers first in late winter and early spring, and leaves are strong. It is an important aroma in early spring in the north. It is a flower-like tree with high ornamental value. The content of chlorogenic acid in the fresh leaves of Lonicera japonica is high, which has important medicinal value. Its fruit has peculiar properties, bright color, sweet and refreshing fruity taste, and rich nutrition. It has high development and application value. Tulip Lonicera has excellent traits and is an excellent...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 尉倩刘安成李淑娟柏国清王庆卢元
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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