Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

hcst gene as a target for diagnosis and treatment of preeclampsia

A preeclampsia, gene technology, applied in the field of biomedicine, can solve problems such as abnormal expression of multiple genes

Active Publication Date: 2020-06-09
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many preeclampsia-related genes have been found, such as the angiotensinogen (AGT) gene (Ward K, Hata A, Jeunemaitre X, et al. A molecular variant of angiotensiongen associated with preeclampsia. NatureGenet, 1993, 4: 59- 61.), prothrombin gene [Kupferminc MJ, EldorA, Steinman N, et al.Increased frequency of genetic thrombophilia in women with complications of pregnancy.N Engl J Ned, 1999,340:9-13], tumor necrosis factor a ( TNF a) gene [Rinehart BK, Terrone DA, Lagoo-Deenadayalan S, et al. Expression of the placental cytokines tumor necrosis factor alpha, interleukin 1beta, and interlukin 10 is increased in preeclampsia. Am J Obstet Gynecol, 1999, 181(4): 915 -920.], etc., but because its occurrence and development involve abnormal expression of multiple genes, more in-depth research is still needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • hcst gene as a target for diagnosis and treatment of preeclampsia
  • hcst gene as a target for diagnosis and treatment of preeclampsia
  • hcst gene as a target for diagnosis and treatment of preeclampsia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Screening for Abnormally Expressed Genes in the Placental Tissue of Patients with Preeclampsia

[0063] 1. Organization collection

[0064] The placental tissues of 5 patients with preeclampsia who were delivered in the obstetrics and gynecology department of the hospital were collected, and 5 cases of placental tissues of late-pregnancy pregnant women with normal blood pressure who underwent surgical termination of pregnancy due to social factors or pelvic abnormalities were selected as the normal control group. Multiple pregnancy, infectious diseases, chemical drug dependence, maternal smoking, fetal congenital malformations and other pregnancy complications and complications were all excluded, and the gestational age was strictly verified according to the B-ultrasound data in the first trimester, and the number of days of pregnancy was calculated. Sign the informed consent form before collecting the specimens. The diagnosis and grading standards of preecla...

Embodiment 2

[0101] Example 2 Large sample verification of expression of differentially expressed genes at the transcriptional level

[0102] 1. Organization collection

[0103] According to the standard of Example 1, 35 cases of placental tissues of preeclampsia patients and 40 cases of placental tissues of normal pregnant women were collected.

[0104] 2. Tissue RNA extraction and identification

[0105] Step is with embodiment 1.

[0106] 3. Design and preparation of primers

[0107] The primer sequences for qRT-PCR detection of HCST gene expression and the primer sequences for qRT-PCR amplification of the internal reference GAPDH were designed and synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., and their sequence information was entered into NCBI after searching the UCSC database The primers are designed by the software and confirmed by blast in the gene bank.

[0108] HCST gene primers:

[0109] Upstream primer: 5'-GGTCACATCCTCTTTCCTG-3' (SEQ ID NO.1);

[0110] Downst...

Embodiment 3

[0133] Embodiment 3 promotes HCST gene expression

[0134] 1. Construction of HCST gene expression vector

[0135] Amplification primers were designed according to the coding sequence of the HCST gene (as shown in SEQ ID NO.5). The coding sequence of the full-length HCST gene was amplified from the cDNA library of adult fetal brain (clontech company, catalog number: 638831). The obtained recombinant vector pcDNA3.1-HCST was connected to the eukaryotic cell expression vector pcDNA3.1 double-digested with enzymes BamHI and XhoI for subsequent experiments.

[0136] 2. Culture of placental trophoblast cells

[0137] JEG-3 cells were placed in DMEM high pond medium containing double antibody and 10% fetal bovine serum at 37°C, 5% CO 2 Cultured in an incubator, the culture medium was changed every 24 hours, and the cells were passaged once every 48 hours. Cells in the logarithmic growth phase were used for subsequent experiments.

[0138] 3. Cell transfection

[0139] Lipofect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
cover factoraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biomedicine and discloses application of HCST (hematopoietic cell signal transducer) gene as a target to diagnose and treat preeclampsia. It is proved by the experiment that HCST gene has significant differences of expression in normal placenta tissue and preeclampsia placenta tissue. According to relevancy of HCST gene to preeclampsia, HCST gene may be used as a novel molecular marker to clinically diagnose preeclampsia and may also be used as a novel target to preeclampsia drugs.

Description

technical field [0001] The invention relates to the field of biomedicine, more specifically, the invention relates to the application of HCST gene in the preparation of products for diagnosis and treatment of preeclampsia. Background technique [0002] Preeclampsia is one of the main diseases that cause increased maternal and child morbidity and mortality in the field of obstetrics. It has obvious genetic predisposition, and its pathophysiological changes involve multi-system and multi-organ lesions. The etiology and pathogenesis have not yet been fully elucidated. , has always been the focus of obstetrics research. The change of gene expression is the core mechanism to regulate the process of cell life activities. Abnormal metabolic processes or pathological changes, whether controlled by a single gene or a multi-gene system, are essentially caused by differential expression of genes, so they can regulate blood pressure, The genes of body fluid volume, placental growth, th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P15/00A61P9/12
CPCA61K45/00A61P9/12A61P15/00C12Q1/6883C12Q2600/158
Inventor 孙耀兰张冬梅
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com