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SNP marker composition related to brain radiation injury caused by radiotherapy and application thereof

A radioactive brain injury and marker technology, applied in the fields of genetic engineering and oncology, can solve problems such as unreported

Active Publication Date: 2018-10-30
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no reports on the genetics of radiation-induced brain injury

Method used

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  • SNP marker composition related to brain radiation injury caused by radiotherapy and application thereof
  • SNP marker composition related to brain radiation injury caused by radiotherapy and application thereof
  • SNP marker composition related to brain radiation injury caused by radiotherapy and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] Example 1 Screening of SNP markers

[0139] 1. Collection of samples and collation of data

[0140] From 2005 to 2007, a large number of blood samples were collected from the Biobank of Sun Yat-Sen University Cancer Hospital. After sorting out the sample information, 1082 samples that met the following criteria were selected for genome-wide microarray scanning:

[0141] Inclusion criteria:

[0142] (1) Nasopharyngeal carcinoma patients with clear pathological diagnosis and clinical stage;

[0143] (2) The expected survival is greater than 6 months;

[0144] (3) Functional status score (KPS) ≥ 70 points;

[0145] (4) Age 18-80 years old;

[0146] (5) Have complete medical records (medical history, physical examination, related examinations, previous treatments);

[0147] (6) Complete radiation therapy and conduct regular MR image review;

[0148] (7) Participate voluntarily and sign the informed consent form.

[0149] At the same time, patients with any of the follo...

Embodiment 2

[0173] Example 2 TaqMan (Applied Biosystems) genotyping detection of a single SNP

[0174] The loci associated with radiation-induced brain injury found in Example 1 through whole-genome scanning were detected and verified in the original sample, and the Sequenom MassARRAY genotyping method was used. The specific steps were as follows:

[0175] 1. The specific steps are:

[0176] (1) Collect 3 mL of peripheral venous blood with an EDTA anticoagulant tube, centrifuge at 3500 r / min for 10 minutes, and then aspirate the plasma.

[0177] (2) Remove red blood cells: add an equal volume of red blood cell lysate (10 mmol / L TrispH7.6; 5 mmol / L MgCl 2 ; 10 mmol / L NaCl), mix well by inverting up and down, centrifuge at 4000r / min for 10 minutes, and discard the supernatant. Then add 5 mL of erythrocyte lysate, mix well by inverting up and down, centrifuge at 4000r / min for 10 minutes, and discard the supernatant.

[0178] (3) Lyse nucleated cells: add 1mL leukocyte lysate (50mmol / L Tri...

Embodiment 3

[0201] Example 3 Kit for Predicting the Risk of Radiation Brain Injury Caused by Tumor Radiotherapy

[0202] The production and operation process of the SNP kit is based on the Sequenom MassARRAY genotyping detection technology.

[0203] 1. The kit contains specific amplification primers and specific extension primers for 15 SNPs:

[0204] The amplification primer sequence of rs1501803 is SEQ ID No: 1-2;

[0205] The amplification primer sequence of rs13166368 is SEQ ID No: 4-5;

[0206] The amplification primer sequence of rs2295767 is SEQ ID No: 7-8;

[0207] The amplification primer sequence of rs6977424 is SEQ ID No: 10-11;

[0208] The amplification primer sequence of rs6988369 is SEQ ID No: 13-14;

[0209] The amplification primer sequence of rs1173107 is SEQ ID No: 16-17;

[0210] The amplification primer sequence of rs1410079 is SEQ ID No: 19-20;

[0211] The amplification primer sequence of rs11017797 is SEQ ID No: 22-23;

[0212] The amplification primer seque...

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Abstract

The invention discloses an SNP marker composition related to onset risk of brain radiation injury caused by tumor radiotherapy and application thereof. The SNP marker composition comprises 15 SNP loci. Meanwhile, a kit for predicating the onset risk of brain radiation injury caused by tumor radiotherapy is prepared, the kit is adopted to detect types of SNP markers, and the onset risk of onset risk of patients suffering from nasopharyngeal carcinoma can be more comprehensively and accurately detected estimated through clinic information of the patients. Through the adoption of the SNP marker composition to clinical applications, and adoption of protective measures in advance to high-risk patients, the SNP marker composition is beneficial to implementation of individualized treatment to thepatients, and improves the long-term survival quality of the patients suffering from nasopharyngeal carcinoma. Meanwhile, the SNP marker composition can provide a method and tactical reference for the predication of risk of normal tissue injury caused by other radiotherapies.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and oncology, more specifically, a group of SNP markers related to radiation-induced brain injury caused by radiotherapy and applications thereof. Background technique [0002] Radiotherapy is the preferred treatment for nasopharyngeal carcinoma, and radiation-induced brain injury is one of the most serious late adverse reactions in patients with nasopharyngeal carcinoma. This adverse reaction is often irreversible, and its clinical symptoms include dizziness, headache, memory loss, cognitive dysfunction, etc., which greatly affect the quality of life of patients. Once radiation-induced brain injury occurs, treatment is quite difficult. Clinically, hormones, gangliosides or citicoline and other drugs are generally given, or hyperbaric oxygen or surgical treatment. In recent years, it has been found that bevacizumab and nerve growth factor also have certain curative effects. Most of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886C12N15/11
CPCC12Q1/6883C12Q1/6886C12Q2600/112C12Q2600/156
Inventor 贾卫华王曈旻沈国平薛文琼
Owner SUN YAT SEN UNIV
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