Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A preparation method of platelet lysate rich in CD41+ and CD81+ microcapsules

A platelet lysate, CD81 technology, applied in medical preparations containing active ingredients, blood/immune system cells, pharmaceutical formulations, etc., can solve problems such as low content of microcapsules, failure to achieve platelet regeneration and repair function, etc.

Active Publication Date: 2021-09-14
天晴干细胞股份有限公司
View PDF29 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used clinical method is to use thrombin and calcium ions to activate, and this method releases less than 10 μg / mL of CD41+ and CD81+ microcapsules with regeneration and repair ability in platelet secretions, and microcapsules in platelet lysate The content of cysts is small, so the optimal state of platelet regeneration and repair function has not been realized

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of platelet lysate rich in CD41+ and CD81+ microcapsules
  • A preparation method of platelet lysate rich in CD41+ and CD81+ microcapsules
  • A preparation method of platelet lysate rich in CD41+ and CD81+ microcapsules

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0019] DETAILED DESCRIPTION a: a rich source of the present embodiment, CD41 +, platelet lysate prepared microcapsules were CD81 + perform the following steps:

[0020] A, with a focusing device of extracorporeal shock wave at a frequency of 15Hz, fluence 0.15 ~ 0.20mJ / mm 2 , At room temperature or impact platelet rich plasma and platelet-rich saline 200-500 times, incubated for 10-15 min at 37 °;

[0021] Second, and then to a frequency 15Hz, fluence 0.20 ~ 0.25mJ / mm 2 , The impact of 700-1000 times at room temperature, incubated for 20-30 min at 37 °;

[0022] Three, then the frequency of 15Hz, fluence 0.25 ~ 0.30mJ / mm 2 , 1500-2000 impact times at room temperature, 37 degrees for 10 minutes to obtain a suspension;

[0023] Fourth, the step of a suspension into cryovials, frozen in liquid nitrogen for 20-40 min, shaking water bath after removal melting operation is repeated 4-6 times and then centrifuged and the supernatant, then the supernatant was filtered with a syringe...

specific Embodiment approach 2

[0030] DETAILED Embodiment 2: Embodiment of the present embodiment and the exemplary embodiment except that a: a step in platelet rich plasma or platelet rich platelet concentration of physiological saline (6 ~ 15) × 10 5 A / μL. Other specific embodiments of the same one or two.

specific Embodiment approach 3

[0031] DETAILED Embodiment 3: Embodiment of the present embodiment and the exemplary embodiment one or two exceptions: Step I, II and III are incubated at 37 [deg.] C to CO 2 Incubator made. DETAILED the same manner as embodiment one or two.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

A method for preparing platelet lysates rich in CD41+ and CD81+ microcapsules. The present invention relates to a method for preparing platelet lysates rich in CD41+ and CD81+ microcapsules. The purpose of the invention is to increase the content of CD41+ and CD81+ in platelet lysates The microcapsules of the present invention increase the formation and release of cytokines, CD41+, and CD81+ microcapsules in the platelets through shock wave grading. The concentration of microcapsules contained in the platelet lysate of the present invention is 120 μg / mL, the total protein of microcapsules is 7-9 μg / mL, 69.33±2.33% of the microcapsules are concentrated in the range of 30-150nm, and 15.10±3.20% of the microcapsules The particle size is concentrated in the range of 150-300nm, and a large amount of platelet cytokines can be released. The invention is applied in the field of biotechnology.

Description

Technical field [0001] The present invention relates to a method of preparing platelet lysate microcapsules CD81 + enriched CD41 +,. Background technique [0002] Platelets is seen in a very long time is non-functional cell fragments in the blood, until 1882, the Italian physician Bizzozero found that they play an important role in hemostasis after vascular injury, platelets was first proposed name has since opened Research platelet coagulation, hemostasis, the maintenance history of the integrity of the capillary wall. Now people have realized that an important function of platelets. [0003] Platelets detached from the bone marrow mature megakaryocytes cytoplasm cytoplasmic cleaved pieces having biological activity, small size, a diameter of 2-3 microns, no nuclei. Having a specific platelet morphology and biochemical composition, there is a relatively constant quantity in normal blood (e.g., human platelet number is 10 to 300,000 per cubic millimeter). Platelets in the resting...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/078A61K38/19A61K35/19A61P17/02
CPCA61K35/19A61K38/19A61P17/02C12N5/0644
Inventor 张怡牛春玮吕秀明刘艳青
Owner 天晴干细胞股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com