A polypeptide having both anti-oxidation and aggregation-inhibiting properties of Aβ42 and its application and gene encoding the polypeptide

An anti-oxidative and characteristic technology, applied in the field of peptides, can solve problems such as organ damage and neuron function damage, achieve major social and economic benefits, good antioxidant effects, and improve medical conditions.

Active Publication Date: 2021-07-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of ROS production and accumulation will obviously cause organ damage, resulting in the inability of neurons to perform normal aerobic metabolism to obtain energy and severely damage their functions.

Method used

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  • A polypeptide having both anti-oxidation and aggregation-inhibiting properties of Aβ42 and its application and gene encoding the polypeptide
  • A polypeptide having both anti-oxidation and aggregation-inhibiting properties of Aβ42 and its application and gene encoding the polypeptide
  • A polypeptide having both anti-oxidation and aggregation-inhibiting properties of Aβ42 and its application and gene encoding the polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Polypeptide WN-5, which has anti-oxidation and anti-Aβ42 aggregation properties, was synthesized by peptide solid-phase synthesis, as follows:

[0036] Using the standard Fomc scheme, Wang resin was selected, and the peptide chain was extended from the C-terminal to the N-terminal one by one according to the sequence characteristics of the amino acid sequence Trp-Pro-Pro-Lys-Asn. The amount of each amino acid was 0.1mol, and 0.3mol of Fmoc was added for protection. For amino acids, HOBt is added to activate and protect the carboxyl groups of amino acids in each step of condensation, and each step of condensation is treated with 20% piperidine / N,N-dimethylformamide (DMF) solution (15ml / g) for 20min to remove the Fmoc protecting group; peptide After the side chain is synthesized, add the peptide chain containing the resin to the dichloromethane: trifluoroacetic acid mixture with a volume ratio of 99:1 to cut the peptide chain from the resin; add the peptide again to the vo...

Embodiment 2

[0039] Determination of Oxidative Free Radical Absorption Capacity of Synthetic Polypeptide WN-5

[0040] Oxygen radical absorbance capacity (ORAC) is a method for evaluating antioxidant capacity based on the hydrogen atom transfer mechanism, which is considered to be close to the antioxidant function of organisms. The ORAC reaction was carried out in a 75mM phosphate buffer solution environment at 37°C, and the synthesized polypeptide WN-5, reduced glutathione (GSH), fluorescein and AAPH free radicals were all dissolved in a 75mM phosphate buffer solution, in which The pH value of the solution was 7.4, and the final concentrations were 1.30 mM, 3.38 mM, 78 nM and 9.95 mM, respectively. Trolox was used as a standard antioxidant, and its final concentration gradient was divided into 0.5 μM, 1.0 μM, 2 μM and 4.2 μM, and GSH was used as a positive control. The synthesized polypeptide WN-5 and GSH were mixed with fluorescein respectively, and incubated at 37°C for 20 minutes, the...

Embodiment 3

[0045] Determination of DPPH·scavenging activity of synthetic polypeptide WN-5

[0046] DPPH radical scavenging activity is a method to evaluate antioxidant capacity based on electron transfer mechanism. The DPPH free radical scavenging activity reaction was carried out at 37°C. The synthesized polypeptide WN-5, ascorbic acid and DPPH were respectively dissolved in 95vol% ethanol, and the concentrations (4mM, 8mM, 12mM, 16mM and 20mM) were accurately measured with a pipette. ) 100 μL of synthetic peptide and ascorbic acid solution were put into a 96-well ELISA plate, then 100 μL of DPPH solution was added, the final concentration of DPPH was 0.2 mM, and the solution was vibrated for 30 s in a microplate reader, incubated for 15 min, and then the solution was detected at 517 nm for absorbance value, the DPPH radical scavenging activity of synthetic peptides or ascorbic acid was calculated as follows:

[0047] DPPH·clearance capacity (%)=[(A control -A sample ) / A control ]×1...

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Abstract

The invention discloses a polypeptide having the properties of anti-oxidation and Aβ42 aggregation inhibition, its application and the gene encoding the polypeptide. The amino acid sequence of the polypeptide having anti-oxidation and anti-Aβ42 aggregation properties of the present invention is Trp-Pro-Pro-Lys-Asn. The polypeptide with anti-oxidation and anti-Aβ42 aggregation properties of the present invention has a good anti-oxidation effect, can significantly reduce the expression of Aβ42 aggregation points in cells, effectively inhibits Aβ42 aggregation, and is applied to the preparation of anti-Aβ42 protein aggregation drugs Or food, or used in the preparation of drugs or food for the prevention or treatment of Alzheimer's disease, which can effectively prevent and treat neurodegenerative diseases including AD diseases, improve the medical conditions of neurodegenerative diseases, and have significant social and economic benefits.

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to polypeptides with anti-oxidation and anti-Aβ42 aggregation properties and applications thereof. Background technique [0002] β-amyloid (amyloid-β, Aβ) is a polypeptide containing 39-43 amino acids produced by the proteolysis of amyloid precursor protein (APP) by β- and γ-secretase. Among them, Aβ42 is the most common subtype of Aβ in the human body and exists in large quantities in Alzheimer's disease patients. It easily aggregates to form the core of Aβ precipitation, triggering neurotoxicity, and is considered as an early-onset biomarker of Alzheimer's disease. [0003] Alzheimer's disease (AD) is a progressive neurodegenerative disease accompanied by dysfunction such as memory impairment, aphasia, agnosia, visual-spatial skill impairment, and executive dysfunction. It is the most common type of senile dementia. form, the etiology of which has not yet been fully elucida...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/11A61K38/08A61P25/28A23L33/18
CPCA23L33/18A61P25/28C07K7/06
Inventor 任娇艳黎青勇
Owner SOUTH CHINA UNIV OF TECH
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