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Geobacillus stearothermophilus strain as well as application and application method thereof

A technology of Geobacillus stearothermophilus and thermophilic fat is applied in the field of Geobacillus stearothermophilus strains, which can solve the problems of reduced activity of decomposing strains, delaying fermentation decomposition time, reducing fertilizer efficiency, etc. the effect of destruction

Inactive Publication Date: 2018-10-23
叶荫人
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the high temperature stage, the average fermentation temperature is higher than 60°C, which will reduce the activity of the decomposing strains, delay the fermentation and decomposition time, and the nitrogen loss during the composting process is relatively serious, and some can even reach 30-50%.
While the organic matter in manure and garbage compost is decomposing, macromolecular nitrogen-containing compounds are converted into low-molecular nitrogen-containing compounds and volatilized, thereby reducing fertilizer efficiency

Method used

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  • Geobacillus stearothermophilus strain as well as application and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 strain screening

[0025] 1) Medium composition and preparation:

[0026] Add 5g of soybean peptone, 5g of sodium chloride, 15g of trypsin, 15g of agar and 1000g of distilled water, add each component into distilled water, stir evenly, adjust the pH to 7.3, and autoclave at 121°C for 15 minutes. Liquid medium without agar.

[0027] 2) Take the organic matter in the fermented compost of poultry and livestock manure at a temperature above 60°C, add 250ml of water, and put it into a high-temperature incubator at 60°C for 24 hours of shaking culture, and the shaking frequency is less than 20 rpm.

[0028] Take 1 mL of the cultured bacterial solution and add it to 9 mL of sterile water, then carry out a 10-fold gradient dilution, and dilute to 10 -2 、10 -3 , 10 -4 , aseptically pipette 0.1 mL of each dilution of the bacterial solution, spread it on the medium plate, each dilution has two parallels, and place it in a 60°C incubator for constant temperature culti...

Embodiment 2

[0031] For the strain R2D2-6.626069934 isolated in Example 1, through the observation of biological characteristics, the morphological identification was carried out, and the results were as follows:

[0032] Cultivate on the culture medium at 60°C for 4 hours, forming large and round white colonies with a diameter of about 1-3mm. Microscopic examination shows that the bacteria are rod-shaped, with an average of 0.8×2.8 μm, and the spores are oval, located at the end of the bacteria.

Embodiment 316

[0033] Example 3 16S rDNA molecular biology identification

[0034] Using the Illumina MiSeq sequencer, the read length can reach 2×250bp, and each reaction can generate 1-8Gb sequencing results.

[0035] Primers:

[0036] GEOBAC-F: 5'-TAAGCGTGAGGTCGGTGGTTC-3', SEQ ID NO: 3

[0037] GEOBAC-R: 5'-GCGCTCTCGGCTTCTTCCTT-3'; SEQ ID NO: 4

[0038] The PCR reaction system (50 μl) was: Premix Taq (Takara Taq TM Version 2.0plus dye) 25 μl, primers GEOBAC-F and GEOBAC-R 2 μl each, template DNA 1.0 μl, deionized water 20 μl.

[0039] The amplification conditions were: pre-denaturation at 95°C for 5 minutes, denaturation at 95°C for 60 seconds, annealing at 55°C for 90 seconds, extension at 72°C for 2 minutes, 35 cycles, and extension at 72°C for 7 minutes to obtain amplified DNA samples. The PCR amplification product was sent to the Biotechnology Development Center of Central University for purification and 16S rDNA sequencing. The sequencing results are shown in SEQ ID NO: 1 and SEQ ...

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Abstract

The invention relates to a geobacillus stearothermophilus strain. The geobacillus stearothermophilus R2D2-6. 626069934 is preserved in CGMCC ( China General Microbiological Culture Collection Center)and has the preservation number of CGMCC NO.15478. The strain can be used for maintaining the activity and realizing mass breeding in the compost fermentation high-temperature period; the fast decomposition of organic matters in the compost fermentation can be promoted; the time required by the composting can be greatly reduced; the complete composting degree is improved; the loss of nitrogen elements caused by high temperature or time factors can be reduced; the national NY525-2012 organic fertilizer industry standard can be reached. The organic waste resource reutilization in poultry and livestock culture industry can be favorably improved; the environment damage of the poultry and livestock culture industry can be greatly reduced; the geobacillus stearothermophilus strain also conformsto the operation rule of rural revitalization and green environment agriculture sustainable environment.

Description

technical field [0001] The invention belongs to the technical field of microbial composting, and relates to a strain of Geobacillus stearothermophilus and its application and application method. Background technique [0002] At present, my country produces more than 150 million tons of meat, eggs and milk every year, and produces about 3.8 billion tons of livestock and poultry manure, and 40% of livestock and poultry waste has not been effectively treated and utilized, which has become a difficult problem in rural environmental governance. The Ministry of Agriculture will accelerate the resource utilization of livestock and poultry waste, promote the green development of animal husbandry, and establish a system for the resource utilization of livestock and poultry breeding waste by 2020, so that the comprehensive utilization rate of livestock and poultry manure in the country will reach more than 75%. At present, in order to deal with these livestock and poultry wastes, many...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G1/00C05F17/00C12R1/07
CPCC05C11/00C05F17/00C12N1/20C12N1/205C12R2001/07C05F5/002C05F5/006C05F11/00C05F11/08C05F3/00Y02W30/40
Inventor 叶荫人
Owner 叶荫人
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