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Method of constructing multi-functional biomimetic modified layer on surface of cardiovascular implant material based on vascular basement membrane structure

An implant material and cardiovascular technology, applied in coatings, pharmaceutical formulations, medical science, etc., can solve the problems of lack of endothelial cells, etc., and achieve the effect of easy-to-operate preparation process, significant effect, and simple preparation process

Active Publication Date: 2018-10-09
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems lie in: 1) the lack of key technologies for smooth muscle cell phenotype and imitation of endothelial cells under fluid shear force on the material surface; 2) immune rejection and inflammatory response during allograft implantation are still urgent problems to be solved in future research The problem
After reviewing the literature, there is no relevant report

Method used

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  • Method of constructing multi-functional biomimetic modified layer on surface of cardiovascular implant material based on vascular basement membrane structure
  • Method of constructing multi-functional biomimetic modified layer on surface of cardiovascular implant material based on vascular basement membrane structure
  • Method of constructing multi-functional biomimetic modified layer on surface of cardiovascular implant material based on vascular basement membrane structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] See figure 1 The first specific embodiment of the present invention is based on the structure of the vascular basement membrane, adopting the patterned dual cell culture-desorption mode to biomimize the construction of a multifunctional modified layer on the surface of stainless steel. The steps are:

[0037] A. Prepare a molecular weight of 5×10 on the polished stainless steel surface 5 DA hyaluronic acid (HA) stripe-like micropatterns, the stripe width of HA is 100nm, and the stripe width of bare material is 200nm, ready for use;

[0038] B. Preparation of patterned vascular smooth muscle extracellular matrix on the surface of stainless steel: the vascular smooth muscle cells passaged for 3 generations were divided into 5×10 4 The density of pieces / ml is planted on the surface of the micro-pattern described in step A, at 37℃, the volume percentage concentration is 5% CO 2 Cultivate for 3 days under the standard culture conditions of HA. In this step, the contractile smooth ...

Embodiment 2

[0042] Based on the structure of the vascular basement membrane, a patterned dual-cell culture-desorption mode is used to biomimically build a multifunctional modified layer on the titanium surface. The steps are as follows:

[0043] A. The molecular weight is 1×10 prepared on the polished titanium surface 6 DA's hyaluronic acid (HA) striped micro-patterns, the stripe width of HA is 25μm, and the stripe width of bare material is 25μm and 25μm respectively, ready for use;

[0044] B. Preparation of patterned vascular smooth muscle extracellular matrix on titanium surface: The vascular smooth muscle cells of 2 passages were divided into 1×10 5 The density of pieces / ml is planted on the surface of the micro-pattern described in step A, at 37℃, 5% CO 2 Cultivate for 2 days under standard culture conditions of concentration. This step obtains contractile smooth muscle cells with elongated shapes and orderly arrangement after being controlled by HA micropatterns; aspirate the used cell cu...

Embodiment 3

[0048] Based on the structure of the vascular basement membrane, a patterned dual cell culture-desorption mode is used to biomimize the construction of a multifunctional modified layer on the surface of the magnesium alloy. The steps are as follows:

[0049] A. The molecular weight is 4×10 prepared on the surface of magnesium alloy 3 DA's hyaluronic acid (HA) stripe-like micro-patterns, the stripe width of HA is 30μm, and the stripe width of bare material is 20μm respectively, ready to use;

[0050] B. Preparation of patterned vascular smooth muscle extracellular matrix on the surface of magnesium alloy: The vascular smooth muscle cells of the second passage were divided into 1×10 6 The density of pieces / ml is planted on the surface of the micro-pattern described in step A, at 37℃, the volume percentage concentration is 5% CO 2 Cultivate for 1 day under the standard culture conditions of HA. In this step, the contractile smooth muscle cells with elongated shape and orderly arranged ...

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Abstract

The invention discloses a method of constructing a multi-functional biomimetic modified layer on the surface of a cardiovascular implant material based on a vascular basement membrane structure. The method comprises the following steps: firstly preparing an HA striped micro graphic on the surface of the material and sequentially performing culture-stripping mode operation on vascular smooth musclecells / endothelial cells on the surface; through the method, the biomimetic vascular basement membrane structure modified layer composed of an extracellular matrix of the contractile smooth muscle cells and an endothelial extracellular matrix under the action of imitation blood flow shearing force can be obtained; the biomimetic modified layer has the multiple functions of anti-coagulation, endothelialization promotion, macrophage adhesion inhibition, and the like. The invention constructs the multi-functional biomimetic vascular basement membrane structure modified layer with the characteristics of anticoagulation, re-endothelialization and macrophage adhesion inhibition on the surface of the cardiovascular implant material, so that the blood compatibility of the material and the abilityto promote the endothelialization are significantly improved.

Description

Technical field [0001] The invention belongs to the cross-technical field of biomaterial surface engineering and biomimetic manufacturing engineering, and particularly relates to the field of micro-pattern preparation technology and surface modification of cardiovascular implant materials, and specifically relates to a bionic construction on the surface of cardiovascular implant materials based on the structure of blood vessel basement membrane Multifunctional modification layer method. Background technique [0002] So far, the surface modification technology of cardiovascular implant materials has gradually matured and is widely used in clinical practice. However, the surface curative effect of materials used in clinical practice is far from reaching the expected goal. This is mainly because no matter how good the surface is modified, its bionic function cannot match the biological function of the body's own cardiovascular blood contact interface. [0003] Through the bionics on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/28A61L27/06A61L27/04A61L27/50
CPCA61L27/047A61L27/06A61L27/28A61L27/34A61L27/50A61L2400/18A61L2420/02A61L2420/04A61L2420/08C08L5/08
Inventor 李敬安张琨关绍康韩丛珍朱世杰王利国王俊常蕾
Owner ZHENGZHOU UNIV
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