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Acclimatization cultivation method of trichaptum biforme, acclimated trichaptum biforme and application

A cultivation method and technology of domestication and cultivation, applied in the field of artificial domestication of fungi, can solve the problems of non-cultivated domesticated species, domestication and cultivation technology of no vesicles, and small quantity

Active Publication Date: 2018-10-02
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the researches on P. pilosae mainly focus on the activity of fermentation products, and the quantity is not much. There is no report on the domestication and cultivation technology of P. cystophora. Therefore, it has always been regarded as a wild germplasm resource, and there is no cultivated domesticated species.

Method used

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  • Acclimatization cultivation method of trichaptum biforme, acclimated trichaptum biforme and application
  • Acclimatization cultivation method of trichaptum biforme, acclimated trichaptum biforme and application
  • Acclimatization cultivation method of trichaptum biforme, acclimated trichaptum biforme and application

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preparation example Construction

[0044] The preparation method of the original seed culture medium of the present invention comprises mixing the sorghum with gypsum after fully absorbing water (water content 55%-60%), subpackaging in original seed bottles, and sterilizing. In the present invention, the size of the original seed bottle is preferably 100×100 mm. In the present invention, the sterilization is preferably high-temperature sterilization, and the temperature of the high-temperature sterilization is preferably 110-150°C, more preferably 120-135°C, most preferably 121°C. In the present invention, the sterilization time is preferably 10-60 minutes, more preferably 20-40 minutes, most preferably 30 minutes. Inoculate after sterilizing and cooling.

[0045] The inoculation of the present invention is preferably to carry out original seed culture on the original seed culture medium of the multiplied mother seed bacterial block. In the present invention, the preferred 10~15mm of the diameter of the propa...

Embodiment 1

[0057] 1. Isolation and cultivation of wild strains: collect the wild vesicles on the birch (such as figure 1 Shown), after cleaning with sterile water, the tissue is separated and the bacterial meat at the junction of the umbrella handle and the canopy is taken and cultivated on the PDA test tube to obtain the mother species mycelium;

[0058] 2. Propagation and cultivation of mother species: sawdust 25-75g / L, bran 25-75g / L, potato slices 150-250g / L, glucose 18-25g / L, peptone 2-4g / L, anhydrous magnesium sulfate 2 ~4g / L, potassium dihydrogen phosphate 1~2g / L, agar 18~22g / L. Add water to make up to 1000mL, and the pH is natural. Using the plate culture method, inoculate the hyphae discs of 6 mm in diameter into the above-mentioned parent culture medium, and cultivate them in a constant temperature incubator at 25°C. The culture results are as follows: figure 2 shown.

[0059] 3. Original seed culture: The original seed medium is: sorghum 98%, gypsum powder 2%. According to...

experiment example 1

[0064] Compared with the wild fungus, the domesticated Epichaete spp. have certain differences in fruiting body agronomic traits and yield. Observe the shape, color and quality of the caps of the two mushrooms with the naked eye. At the same time, use a vernier caliper to measure the thickness of the caps of the fruiting bodies, the horizontal and vertical diameters of the caps, weigh the weight of a single mushroom, and calculate the mushroom shape index and yield. The results are shown in the table. 1.

[0065] Table 1 Agronomic traits and yield analysis of Epichaete spp.

[0066]

[0067] It can be seen from Table 1 that compared with the wild fungus, the domesticated fruiting body has thicker flesh, brighter color, harder texture, thicker cap, and larger horizontal and vertical diameters, and its agronomic characteristics are better than wild fungi. Moreover, the yield of fruiting bodies is larger, and a large number of fruiting bodies can be obtained from the domestic...

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Abstract

The invention provides an acclimatization cultivation method of trichaptum biforme, and belongs to the technical field of artificial acclimatization of fungi. The method comprises the following steps:firstly, performing isolated culture on wild trichaptum biforme sporocarp, so as to obtain mother strain mycelia; secondly, performing mother strain expanding propagation culture on the mother strainmycelia, so as to obtain propagated mother strain; thirdly, performing original strain culture on a propagated mother strain fungus block, so as to obtain original strain; fourthly, performing cultivated species culture on the original strain, so as to obtain cultivated species; fifthly, performing acclimatization cultivation culture on the cultivated species. The method has the advantages that the mycelia growth speed is high, and the mycelia are good in growing and dense; primordium is produced within 14 to 17d, small sporocarp is formed within 7 to 10d, and normal sporocarp is formed within 10 to 15d, and the yield of sporocarp is high. The trichaptum biforme sporocarp obtained through artificial cultivation is nontoxic, and sporocarp polysaccharide has obvious in vitro anti-hepatoma activity.

Description

technical field [0001] The invention belongs to the technical field of artificial domestication of fungi, and in particular relates to a domestication method of the pilosa fungus, the domesticated pilose fungus and its application. Background technique [0002] my country has a vast territory and rich fungal resources, with more than 3,700 species of wild fungi. Many types of wild mushrooms have unique fragrance and delicious taste. They contain protein, polysaccharides, unsaturated fatty acids, mineral elements, vitamins and other nutrients in their bodies. Many of them have medicinal value, such as anti-tumor, anti-inflammation, anti-virus, Protecting the liver, enhancing immunity, lowering blood sugar, anti-gastric ulcer, anti-aging and anti-aging, preventing and treating arthritis and other pharmacological activities. Trichaptum biforme is a species of the genus Trichaptum biforme belonging to the Basidiomycota Heterobasidiomycetes, Polyporaceae Polyporaceae, which grow...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/36A61K36/07A61P35/00C12R1/645
CPCA61K36/07A61P35/00C12N1/14C12N1/36C12N1/145C12R2001/645
Inventor 王立安姚澜赵立强张金秀
Owner HEBEI NORMAL UNIV
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