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Preparation method and application of a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor

An immunosensor, molybdenum diselenide technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems such as insufficient sensitivity, and achieve the effects of improving sensitivity, improving conductivity, and improving electron transmission capacity

Active Publication Date: 2020-01-10
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the immunological detection methods of clinical serum tumor markers mainly include radioimmunoassay, enzyme-linked immunoassay, time-resolved fluorescence immunoassay, etc., but they cannot avoid the shortcomings of radioactive pollution, time-consuming, and insufficient sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 A method for preparing a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor, the steps are as follows:

[0049] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0050] (2) Take 6 µL, 1.0 mg / mL gold nanorod-loaded aminated graphene dispersion and apply it dropwise on the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0051] (3) Add 6 µL, 8.0 µg / mL tumor marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0052] (4) Continue to add 3 µL, 0.5 mg / mL bovine serum albumin BSA solution dropwise to the surface of the electrode to seal the non-specific active sites on the electrode, rinse the surface of the electrode with ultrapure water, and dry it in a refrigera...

Embodiment 2

[0055] Example 2 A preparation method of a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor, the steps are as follows:

[0056] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0057] (2) Take 6 µL, 2.0 mg / mL gold nanorod-loaded aminated graphene dispersion and apply it dropwise on the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0058] (3) Add 6 µL, 10.0 µg / mL tumor marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0059] (4) Continue to add 3 µL, 1.0 mg / mL bovine serum albumin BSA solution dropwise to the surface of the electrode to seal the non-specific active sites on the electrode, rinse the surface of the electrode with ultrapure water, and dry it in a refrig...

Embodiment 3

[0062] Example 3 A preparation method of a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor, the steps are as follows:

[0063] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0064] (2) Take 6 µL, 3.0 mg / mL gold nanorod-loaded aminated graphene dispersion and apply it dropwise on the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0065] (3) Add 6 µL, 12.0 µg / mL tumor marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0066] (4) Continue to add 3 µL, 2.0 mg / mL bovine serum albumin BSA solution dropwise to the surface of the electrode to seal the non-specific active sites on the electrode, rinse the surface of the electrode with ultrapure water, and dry it in a refrig...

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Abstract

The invention belongs to the technical field of immune analysis and biosensing, and provides a preparation method and application of a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor. The invention utilizes bimetallic Pd@Pt nanoparticles to support amino-functionalized MoSe 2 As a catalytic material, it is used as a marker after being incubated with a detection antibody. At the same time, gold nanorods are used to load amino-functionalized graphene as an electrode modification material to successfully construct a sandwich-type immunosensor, thereby realizing the detection of tumor markers NSE and SCCA. It has the advantages of high sensitivity, strong specificity, and low detection limit, and has important scientific significance and application value for early detection of tumors.

Description

technical field [0001] The invention belongs to the technical fields of nanometer functional materials, immune analysis and biosensing, and relates to a preparation method and application of a platinum-palladium composite molybdenum diselenide-labeled sandwich-type immunosensor. Specifically, Pd@Pt / MoSe is used 2 In order to detect antibody markers, sensitive detection of tumor markers NSE and SCCA was realized. Background technique [0002] Cancer is a disease with high morbidity and high mortality that seriously threatens human health. Although the treatment technology of malignant tumors is constantly improving, so far, early detection, early diagnosis and early treatment of malignant tumors are still the most effective means to treat malignant tumors. Tumor markers are specific substances that exist or are secreted by tumor cells. Most of them exist in malignant tumors. The detection of tumor markers in serum has important scientific significance and application prospe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/574
CPCG01N27/308G01N27/3278G01N33/57484
Inventor 李月云张栓张春燕贾翌雷赵增典
Owner SHANDONG UNIV OF TECH
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