A kind of preparation method of the sandwich type electrochemical immunosensor of cardiac troponin
A technology of cardiac troponin and immunosensor, which is applied in the field of immunoassay and biosensing, can solve the problems of long detection time, complicated operation, radioactive contamination, etc., and achieve the goal of lower detection limit, larger specific surface area, and excellent conductivity Effect
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Embodiment 1
[0050] Embodiment 1 prepares gold nanocubic functionalized graphene oxide dispersion, the steps are as follows:
[0051] (1) Preparation of gold nanocubic dispersion
[0052] Prepare 10 mL of a mixture containing 0.25 mM chloroauric acid and 75 mM cetyltrimethylammonium bromide in a 50 mL round bottom flask, and quickly add 0.5 mL, 10 mM freshly prepared ice boron under magnetic stirring Sodium hydride solution was stirred in a water bath at 27°C for 3 hours to obtain a gold nanoparticle aqueous sol;
[0053] Measure 1.0 mL of the above-prepared gold nanoparticle hydrosol and dilute it to 100 mL with ultrapure water as the gold seed dispersion;
[0054] In a 50 mL centrifuge tube, add 0.1 mL, 10 mM chloroauric acid solution, 2.0 mL, 0.2 M cetyltrimethylammonium bromide solution, 1.5 mL, 0.1 M ascorbic acid, and 0.3 mL of the above-prepared gold seed dispersion solution, diluted to 25 mL with ultrapure water, and shaken at 27 °C for 8 hours in a constant temperature shaking i...
Embodiment 2
[0059] Embodiment 2 prepares gold nanocubic functionalized graphene oxide dispersion, the steps are as follows:
[0060] (1) Preparation of gold nanocubic dispersion
[0061] Prepare 10 mL of a mixture containing 0.25 mM chloroauric acid and 75 mM cetyltrimethylammonium bromide in a 50 mL round bottom flask, and quickly add 0.6 mL of 10 mM freshly prepared ice boron under magnetic stirring Sodium hydride solution was stirred in a water bath at 27°C for 3 hours to obtain a gold nanoparticle aqueous sol;
[0062] Measure 1.0 mL of the above-prepared gold nanoparticle hydrosol and dilute it to 100 mL with ultrapure water as the gold seed dispersion;
[0063] In a 50 mL centrifuge tube, add 0.1 mL, 10 mM chloroauric acid solution, 2.0 mL, 0.2 M cetyltrimethylammonium bromide solution, 1.5 mL, 0.1 M ascorbic acid, and 0.4 mL of the above-prepared gold seed dispersion solution, diluted to 25 mL with ultrapure water, and shaken at 27 °C for 8 hours in a constant temperature shaking...
Embodiment 3
[0068] Example 3 Preparation of gold nanocubic functionalized graphene oxide dispersion, the steps are as follows:
[0069] (1) Preparation of gold nanocubic dispersion
[0070] Prepare 10 mL of a mixture containing 0.25 mM chloroauric acid and 75 mM cetyltrimethylammonium bromide in a 50 mL round bottom flask, and quickly add 0.7 mL, 10 mM freshly prepared ice boron under magnetic stirring Sodium hydride solution was stirred in a water bath at 27°C for 3 hours to obtain a gold nanoparticle aqueous sol;
[0071] Measure 1.0 mL of the above-prepared gold nanoparticle hydrosol and dilute it to 100 mL with ultrapure water as the gold seed dispersion;
[0072] In a 50 mL centrifuge tube, add 0.1 mL, 10 mM chloroauric acid solution, 2.0 mL, 0.2 M cetyltrimethylammonium bromide solution, 1.5 mL, 0.1 M ascorbic acid, and 0.5 mL of the above-prepared gold seed dispersion solution, diluted to 25 mL with ultrapure water, and shaken at 27 °C for 8 hours in a constant temperature shakin...
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