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Preparation method and application of a sandwich-type electrochemical sensor for lung cancer markers

A marker and sensor technology, applied in the field of immunoassay and biosensing, can solve the problems of requiring large-scale instruments and equipment, radioactive pollution, etc., and achieve the effects of good water solubility, catalytic performance, large specific surface area, and excellent electrical conductivity

Inactive Publication Date: 2020-01-14
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection methods for lung cancer markers, such as enzyme-linked immunoassay, radioimmunoassay, etc., but these methods may require large-scale instruments and equipment during the detection process, and there are problems such as radioactive contamination. Therefore, the invention of a highly specific , high sensitivity, fast detection speed, and easy-to-operate lung cancer tumor marker sensor is very important

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 A preparation method of a sandwich-type electrochemical sensor for lung cancer markers, the steps are as follows:

[0044] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0045] (2) Take 6 µL, 1.0 mg / mL polydopamine-functionalized gold nanoparticle dispersion and apply it onto the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0046] (3) Add 6 µL, 8 µg / mL lung cancer marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0047] (4) Continue to add 3 µL, 0.5 mg / mL bovine serum albumin BSA solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0048] (5) Add 6 µL, 0.0001 ~ 100 ng / mL of a series of different concentrations of lung cancer marke...

Embodiment 2

[0050] Example 2 A preparation method of a sandwich-type electrochemical sensor for lung cancer markers, the steps are as follows:

[0051] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0052] (2) Take 6 µL, 2.0 mg / mL polydopamine-functionalized gold nanoparticle dispersion and apply it onto the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0053] (3) Add 6 µL, 10 µg / mL lung cancer marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0054] (4) Continue to add 3 µL, 1.0 mg / mL bovine serum albumin BSA solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0055] (5) Add 6 µL, 0.0001 ~ 100 ng / mL of a series of different concentrations of lung cancer mark...

Embodiment 3

[0057] Example 3 A preparation method of a sandwich-type electrochemical sensor for lung cancer markers, the steps are as follows:

[0058] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0059] (2) Take 6 µL, 3.0 mg / mL polydopamine-functionalized gold nanoparticle dispersion and apply it onto the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0060] (3) Add 6 µL, 12 µg / mL lung cancer marker capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0061] (4) Continue to add 3 µL, 1.5 mg / mL bovine serum albumin BSA solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0062] (5) Add 6 µL, 0.0001 ~ 100 ng / mL of a series of different concentrations of lung cancer mark...

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Abstract

The invention belongs to the technical field of immune analysis and biosensing, and provides a preparation method and application of a sandwich-type electrochemical sensor for lung cancer markers. The present invention utilizes the bimetallic nanomaterial Au@Pt with core-shell structure to functionalize nitrogen-doped graphene-loaded Cu 2+ As a catalytic material, it is used as a marker after being incubated with a detection antibody, and polydopamine-functionalized gold nanoparticles are used as an electrode modification material, thereby realizing the detection of lung cancer markers CEA, CA125, and SCCA, with high sensitivity and specificity. The detection limit is low, and it has important scientific significance and application value for the early detection of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of immune analysis and biosensing, and relates to a preparation method and application of a sandwich-type electrochemical sensor for lung cancer markers. Specifically, new nano-functional materials are used as catalysts to label detection antibodies to achieve sensitive detection of lung cancer markers CEA, CA125, and SCCA. Background technique [0002] Lung cancer has a high incidence rate, is difficult to detect, and the tumor grows and metastasizes rapidly, becoming one of the malignant tumors that threaten the health and life of the population most. Therefore, it is very important to realize the sensitive detection of lung cancer markers for the early detection and early treatment of lung cancer. At present, there are many detection methods for lung cancer markers, such as enzyme-linked immunoassay, radioimmunoassay, etc., but these methods may require large-scale instruments and equipment during the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N33/574
CPCG01N27/308G01N27/3277G01N27/3278G01N33/574G01N33/57423G01N33/57473G01N33/57484G01N2800/12
Inventor 李月云吕慧张晓波张春燕张栓王平董云会
Owner SHANDONG UNIV OF TECH
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