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A strontium-doped porous silk fibroin microsphere and its preparation method

A technology of porous microspheres and porous filaments, which is applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problems of unstable performance of prepared materials, incomplete research on structure and morphology, etc., and achieve the effect of low cost.

Active Publication Date: 2020-08-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the main problems of existing micro-carriers in the application of tissue engineering, such as the unstable properties of the prepared materials and the incompleteness of the research on the structure and morphology, and combines the advantages and characteristics of silk fibroin to provide a kind of microcarrier based on silk fibroin and strontium chloride. Main material, strontium-doped silk fibroin-based porous microspheres prepared by emulsion method combined with freeze-drying technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate them into 2% (w / v) silk fibroin solution;

[0024] 2) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0025] 3) Take 40ml of petroleum ether in a new 100ml beaker, add 0.7ml of span-80, and mix the two evenly;

[0026] 4) Mix 10ml of silk fibroin solution and 0.01g of strontium chloride evenly, add the solution in 3), and stir to form a uniform spherical shape;

[0027] 5) Pour the solution of 4) into the pre-cooled petroleum ether in 2) quickly, and then place it at -50°C to stand for precipitation;

[0028] 6) Remove the petroleum ether in the solution of 5) in a freezing atmosphere to obtain strontium-doped silk fibroin microspheres containing ice crystals, freeze the silk fibroin microspheres, and then obtain dry strontium-doped porous silk by freeze-drying protein microspheres.

[0029] The composition of the obtained porous microsphere ...

Embodiment 2

[0031] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate them into 4% (w / v) silk fibroin solution;

[0032] 2) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0033] 3) Take 40ml of petroleum ether in a new 100ml beaker, add 0.7ml of span-80, and mix the two evenly;

[0034] 4) Mix 10ml of silk fibroin solution and 0.01g of strontium chloride evenly, add the solution in 3), and stir to form a uniform spherical shape;

[0035] 5) Pour the solution of 4) into the pre-cooled petroleum ether in 2) quickly, and then place it at -50°C to stand for precipitation;

[0036] 6) Remove the petroleum ether in the solution of 5) in a freezing atmosphere to obtain strontium-doped silk fibroin microspheres containing ice crystals, freeze the silk fibroin microspheres, and then obtain dry strontium-doped porous silk by freeze-drying protein microspheres.

Embodiment 3

[0038] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate them into 6.5% (w / v) silk fibroin solution;

[0039] 2) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0040] 3) Take 40ml of petroleum ether in a new 100ml beaker, add 0.7ml of span-80, and mix the two evenly;

[0041] 4) Mix 10ml of silk fibroin solution and 0.01g of strontium chloride evenly, add the solution in 3), and stir to form a uniform spherical shape;

[0042] 5) Pour the solution of 4) into the pre-cooled petroleum ether in 2) quickly, and then place it at -50°C to stand for precipitation;

[0043] 6) Remove the petroleum ether in the solution of 5) in a freezing atmosphere to obtain strontium-doped silk fibroin microspheres containing ice crystals, freeze the silk fibroin microspheres, and then obtain dry strontium-doped porous silk by freeze-drying protein microspheres.

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Abstract

The invention discloses a preparation method of a strontium-doped porous silk fibroin microsphere. The strontium-doped porous silk fibroin microsphere is prepared from silk fibroin and strontium chloride, wherein the porosity of the porous microsphere is 80 percent or above, the aperture is 5 to 40mu m, and the weight of the strontium chloride accounts for 5 to 38 percent of total weight. The silkfibroin disclosed by the invention is used as a natural protein module, is widely used for the health-care food industry and has no stimulating effect on a human body; strontium is a trace element which is essential for the human body, has the capabilities of osteogenic induction and the like, and can realize the effect of improving bone repair; meanwhile, the silk fibroin as a main material fora cell carrier can simulate protein components of an extracellular matrix. According to the strontium-doped porous silk fibroin microsphere prepared by the preparation method disclosed by the invention, slow release of strontium can be realized, and the problems that growth factors are obtained from animal body for use and the like are effectively solved.

Description

technical field [0001] The invention relates to a method for preparing porous silk fibroin microspheres doped with strontium, which belongs to the technical field of biomedical materials. Background technique [0002] Bone and cartilage tissue play a very important role in supporting and protecting the human body. The traditional bone repair method is to use autologous bone and allogeneic bone for bone transplantation. This method is not only limited by bone supply resources, but also expensive. The use of allogeneic bone is also prone to adverse reactions such as immune rejection. With the development of tissue engineering and regenerative medicine, it has achieved great success in bone regeneration, and the regenerated bone has normal functions and biological activities. In the process of bone repair, it is necessary to construct three-dimensional scaffolds loaded with cells and growth factors, such as porous sponge scaffolds, fiber mesh scaffolds, and hydrogels. Compared...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/50A61L27/22
CPCA61L27/227A61L27/50A61L27/54A61L27/56A61L2300/102A61L2300/602A61L2430/02
Inventor 杨明英雷芳
Owner ZHEJIANG UNIV
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