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Time resolution fluorescent test strip for detecting permethrin and application of time resolution fluorescent test strip

A technology of time-resolved fluorescence and permethrin, applied in measuring devices, analytical materials, instruments, etc., can solve the problems of unsuitable basic-level laboratory batches, rapid detection of samples, difficult batch detection of samples, complex detection steps, etc., to achieve easy The effects of promotional use, high sensitivity, and short detection time

Inactive Publication Date: 2018-09-14
NORTH CHINA UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The accuracy of the instrument detection method is high, but the instrument is expensive, requires professional technicians, the detection steps are complicated, and it is difficult to detect samples in batches. It is not suitable for batch and rapid detection of samples in grassroots laboratories.

Method used

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  • Time resolution fluorescent test strip for detecting permethrin and application of time resolution fluorescent test strip
  • Time resolution fluorescent test strip for detecting permethrin and application of time resolution fluorescent test strip
  • Time resolution fluorescent test strip for detecting permethrin and application of time resolution fluorescent test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of permethrin detection test strips

[0040] The preparation method of this test strip mainly comprises the following steps:

[0041] 1) Prepare a sample absorption pad sprayed with a permethrin monoclonal antibody-time-resolved fluorescent marker;

[0042] 2) preparing a reaction membrane with a detection line coated with a permethrin hapten-carrier protein conjugate and a quality control line coated with an anti-rabbit antibody;

[0043] 3) Assemble the sample absorbent pad, reaction membrane and water absorbent pad prepared in 1) and 2) into a test strip.

[0044] The following step-by-step detailed description:

[0045] 1. Preparation of permethrin hapten

[0046] (1) Dissolve 24.3g of 3-(4-nitrophenoxy)benzaldehyde in 100ml of anhydrous methanol, stir and dissolve at room temperature, add 0.4g of sodium borohydride in batches, react at room temperature for 5h, evaporate the solvent to dryness, and Analysis and purification, to obtain 23.3g...

Embodiment 2

[0067] Example 2 Detection of Permethrin Residues in Samples

[0068] 1. Sample processing

[0069] (1) Apple pretreatment method.

[0070] Homogenize the sample with a homogenizer; weigh (2.00±0.05)g homogenized apple sample into a 10mL polystyrene centrifuge tube, add 4mL 0.01M PBS, 2mL methanol, and vortex for 5min with a vortexer; room temperature Centrifuge at 4000r / min for 5min; take 200μL of the supernatant, add it to 800μL of 0.01M PBS, mix thoroughly, and take 50μL of it for analysis.

[0071] (2) Corn pretreatment method.

[0072] Homogenize the sample with a homogenizer; weigh (2.00±0.05)g homogenized corn sample into a 10mL polystyrene centrifuge tube, add 4mL 10% sodium chloride and 2mL methanol respectively, and vibrate with a vortexer for 5min; Centrifuge at 4000r / min for 5min at room temperature; take 100μL of the supernatant, add it to 900μL of 0.01M PBS, mix thoroughly, and take 50μL of it for analysis.

[0073] (3) Vegetable / tea pretreatment method.

[...

Embodiment 3

[0082] Embodiment 3 sample detection example

[0083] 1. Detection limit test

[0084] Take blank apple / corn / tea / tomato / spinach / cabbage samples, add permethrin to the final concentration of 0.5ng / ml, 1ng / ml, 5ng / ml, 10ng / ml, 15ng / ml, 25ng / ml, 30ng / ml, 35ng / ml, take a test strip for detection, and repeat the measurement three times for each sample.

[0085] When testing samples such as apple / corn / tea / tomato / spinach / cabbage with test strips, determine the detection limit range according to the results displayed on the test strips, indicating that the detection limit range of the test strips: 1ng / ml-30ng / ml.

[0086] 2. Sample precision and accuracy test

[0087] The recovery rate was used as the accuracy evaluation index, and the relative standard deviation (RSD%) of the test results of repeated determination of a certain concentration sample was used as the precision evaluation index. The calculation formula is: recovery rate (%)=actual measured value / theoretical value×100%,...

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Abstract

The invention discloses a time resolution fluorescent test strip for detecting permethrin and application of the time resolution fluorescent test strip. The test strip comprises a sample absorption mat, a reaction membrane and a water absorption mat, wherein the reaction membrane is disposed in the middle, and the sample absorption mat and the water absorption mat are respectively in lap joint onthe left end and the right end of the reaction membrane; the sample absorption mat is provided with a sample feeding area and a microsphere area, and the microsphere area is provided with permethrin time resolution fluorescent microspheres; and the reaction membrane is provided with a detection line (line T) and a quality control line (line C), the line T is connected with an upper coated permethrin antigen (coating antigen), and the line C is coated with an anti-rabbit antibody. The method also provides a method for detecting permethrin residue in samples such as apples, maize, tealeaves, tomatoes, spinach, Chinese cabbages and the like by using the permethrin test strip. The test strip provided by the invention has the characteristics of simple operation, high sensitivity, quantitative detection, rapid detection, low cost and the like, and is suitable for screening and on-site monitoring a great amount of samples.

Description

technical field [0001] The invention relates to a time-resolved fluorescent test strip for detecting permethrin and its application, belongs to the technical field of time-resolved fluorescent immunoassay (TRFIA), and is used for permethrin in samples such as apples, corn, tea leaves, tomatoes, spinach, and cabbage. Esters content detection. Background technique [0002] Permethrin, also known as permethrin, insecticide, permethrin, is a pyrethroid insecticide without cyano structure. As the first unstable insecticide suitable for the control of agricultural pests among the pyrethroid insecticides, permethrin has strong contact and stomach poisoning effects, and can be used to control cotton, vegetables, tea, and fruit trees. pests, especially for the control of sanitary pests. Compared with organochlorine pesticides, pyrethroid pesticides have less pollution to the environment, but they can enter the body through the food chain, thereby causing reproductive, immune and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/533G01N33/543
CPCG01N33/531G01N33/533G01N33/54306G01N33/558
Inventor 张静王晓磊
Owner NORTH CHINA UNIVERSITY OF TECHNOLOGY
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