Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Furan metabolite hapten and artificial antigen and their application in quantitative fluorescence immunochromatography

An artificial antigen and fluorescence quantitative technology, applied in the field of immunochemistry, can solve the problems that the colloidal gold method cannot meet the national detection standards, cannot meet the rapid screening, and the detection time is long, so as to achieve consistent density, improve immunogenicity, and improve efficiency. price effect

Inactive Publication Date: 2018-09-14
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD +2
View PDF10 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing enzyme-linked immunoassay method has high sensitivity and high throughput, it also requires professional operation, and the detection time is long, and it also cannot meet the needs of rapid on-site screening; while the colloidal gold method has high throughput, It has the advantages of simple operation, short detection time and no need for professional and technical personnel to operate, but its poor sensitivity also makes the colloidal gold method unable to meet the requirements of national detection standards

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Furan metabolite hapten and artificial antigen and their application in quantitative fluorescence immunochromatography
  • Furan metabolite hapten and artificial antigen and their application in quantitative fluorescence immunochromatography
  • Furan metabolite hapten and artificial antigen and their application in quantitative fluorescence immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of furan metabolite hapten, its preparation method is as follows:

[0052] (1) Take 4.8g (28.9mmol) of compound I in a 50ml round bottom flask, then add 20ml of acetone, 3.0g (11.5mmol) of compound II and 3.2g (23.0mmol) of potassium carbonate. React at 80°C for more than 5h. After the reaction was complete, remove the solvent under reduced pressure, add 40ml of purified water, extract twice with ethyl acetate, remove the solvent under reduced pressure, and recrystallize to obtain 3.2g of compound III (ethyl acetate / petroleum ether, 1 / 5, v / v) .

[0053] (2) Dissolve 3.2g (7.4mmol) of compound III in 10ml of ethanol, add 6M lithium hydroxide solution to adjust the pH value of the ethanol solution of compound III to 10, react at room temperature for more than 15h, and then add 40ml of purified water to obtain The pH value of the solution was adjusted to 4 by 1M dilute hydrochloric acid, filtered, and dried to obtain 2.3 g of compound IV.

[0054] (3) Dissolve 50...

Embodiment 2

[0056] A kind of furan metabolite hapten, its preparation method is as follows:

[0057] (1) Take 3.8g (23.0mmol) of compound I in a 50ml round bottom flask, then add 20ml of acetone, 3.0g (11.5mmol) of compound II and 2.4g (17.3mmol) of potassium carbonate. Reaction at 90°C for 2h. After completion of the reaction, remove the solvent under reduced pressure, add 40ml of purified water, extract twice with ethyl acetate, remove the solvent under reduced pressure, and purify by column chromatography to obtain 2.4g of compound III (ethyl acetate / petroleum ether, 1 / 5, v / v).

[0058] (2) Dissolve 2.4g (5.6mmol) of compound III in 10ml of ethanol, add 6M lithium hydroxide solution to adjust the pH value of the ethanol solution of compound III to 12, react at room temperature for 26h, and then add 40ml of purified water to obtain The pH value of the solution was adjusted to 5 by 1M dilute hydrochloric acid, filtered, and dried to obtain 1.7 g of compound IV.

[0059] (3) Dissolve ...

Embodiment 3

[0061] A kind of furan metabolite hapten, its preparation method is as follows:

[0062] (1) Take 3.8g (23.0mmol) of compound I in a 50ml round bottom flask, then add 20ml of acetone, 3.0g (11.5mmol) of compound II and 2.4g (17.3mmol) of potassium carbonate. Reaction at 90°C for 2h. After completion of the reaction, remove the solvent under reduced pressure, add 40ml of purified water, extract twice with ethyl acetate, remove the solvent under reduced pressure, and purify by column chromatography to obtain 2.6g of compound III (ethyl acetate / petroleum ether, 1 / 5, v / v).

[0063] (2) Dissolve 2.6g (6.1mmol) of compound III in 10ml of ethanol, add 6M lithium hydroxide solution to adjust the pH value of the ethanol solution of compound III to 11, react at room temperature for 20h, and then add 40ml of purified water to obtain The pH value of the solution was adjusted to 4.5 by 1M dilute hydrochloric acid, filtered, and dried to obtain 1.9 g of compound IV.

[0064] (3) Dissolv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
intra-assay coefficient of variationaaaaaaaaaa
Login to View More

Abstract

The invention discloses furan metabolite hapten and artificial antigen and their application in quantitative fluorescence immunochromatography. The hapten structure has characteristic structures of two articles under test such that the spatial position of the hapten in a carrier protein is more prominent, a recognition site is added, immunogenicity of the small-molecular hapten is effectively improved, and valence of the hapten is also improved. The artificial antigen herein has high sensitivity for the ELISA (enzyme-linked immuno sorbent assay) of furan metabolites. A quantitative fluorescence immunochromatography test strip made with the artificial antigen can cooperate with a detection method of the invention to quickly and conveniently test furan metabolites; the test strip has high sensitivity, high precision and good stability.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry, and in particular relates to a furan metabolite hapten, an artificial antigen and an application thereof in fluorescence quantitative immunochromatography. Background technique [0002] Furans are a class of artificially synthesized antibiotics that share a 5-nitrofuran ring structure, and have antibacterial effects on Gram-positive bacteria, Gram-negative bacteria, and some fungi. Because of its low price, it is widely used in livestock, poultry and fish farming to treat enteritis and coccidiosis caused by Salmonella and Escherichia coli, and is often used as a growth promoter for livestock and aquatic fish and shrimp added to the feed. Nitrofurans mainly include furaltadone, nitrofurazone, nitrofurantoin and furazolidone. Nitrofuran prototype drugs are rapidly metabolized in vivo, and their metabolites are AOZ, AMOZ, AHD, and SEM. Therefore, the detection of metabolites is often used ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D263/26C07D233/80C07C281/14C07K14/76C07K14/765C07K14/795C07K16/44G01N33/68
CPCC07C281/14C07D233/80C07D263/26C07K14/76C07K14/765C07K14/795C07K16/44C07K2319/00G01N33/68
Inventor 李斌石松谢俊平江林峰凌家亮石林花任季玉
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com