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Method for creating corn autotetraploid

A technology of autotetraploid and corn, applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of low success rate and so on

Inactive Publication Date: 2018-09-14
SHENYANG JINSEGU SPECIAL CORN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 2. low success rate
[0011] The technical problem to be solved in this application is to obtain autotetraploid by directly inducing diploid somatic cell chromosome doubling in sexual organs and tissues, and solve the problems caused by the ploidy mosaicism produced by the existing technique of inducing somatic cell chromosome doubling The problem of low success rate, and significantly simplify the breeding procedure and reduce the cost of breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Creation of autotetraploid Shentian 6

[0053] (1) Creation of autotetraploid double-bred line

[0054] Plant the female parent inbred line Shentian 135, on the 4th day of silking, use 0.2% colchicine aqueous solution to chemically induce chromosome doubling by paper paste method. Through field identification and chromosome observation of individual plants and ear rows, Autotetraploid. After further breeding for the seed setting rate, an autotetraploid sweet corn inbred line was selected; the same method was used to select the male parent inbred line Shentian H154 of Shentian 6 into an autotetraploid sweet corn inbred line .

[0055] (2) Preparation of Shentian 6 autotetraploid hybrid

[0056] Using autotetraploid Shentian 135 as the female parent and autotetraploid Shentian H154 as the male parent for seed production, the seeds of the autotetraploid sweet corn Shentian 6 hybrid were made.

[0057] (3) Technical effects

[0058] Compared with diploid Shentian 6, the ...

Embodiment 2

[0060] Example 2: Creation of Autotetraploid Shennuo No. 7

[0061] (1) Creation of autotetraploid double-bred line

[0062] Plant the female parent inbred line Shennuo 509. On the 4th day of silking, use 0.6% of 48% trifluralin aqueous solution to chemically induce chromosome doubling by the dripping method, and pass the field identification of individual plants and ear rows. Observe the chromosome, and obtain the autotetraploid. After further breeding for seed setting rate, the autotetraploid waxy corn inbred line was selected and bred; the male parent inbred line Shennuo 350 of Shennuo No. 7 was selected and bred into an autotetraploid waxy corn inbred line using the same method .

[0063] (2) Preparation of Shennuo No. 7 autotetraploid hybrid

[0064] The seeds of the autotetraploid glutinous corn hybrid Shennuo No. 7 were produced by using the autotetraploid Shennuo 509 as the female parent and the autotetraploid Shennuo 350 as the male parent.

[0065] (3) Technical effects

[0...

Embodiment 3

[0068] Example 3: Creation of Zhengdan 958 autotetraploid

[0069] (1) Creation of autotetraploid double-bred line

[0070] Plant the female parent inbred line Zheng 58, and use 0.6% of 48% trifluralin aqueous solution on the 4th day of silking, and use the dripping method to chemically induce chromosome doubling. Through the field identification and chromosomes of individual plants and ear rows Observe and obtain autotetraploid. After further breeding for seed setting rate, an autotetraploid inbred line was bred; using the same method, Zhengdan 958's male parent inbred line Chang 7-2 was selected and bred into an autotetraploid inbred line.

[0071] (2) Preparation of Zhengdan 958 homotetraploid hybrid

[0072] With autotetraploid Zheng 58 as the female parent and autotetraploid Chang 7-2 as the male parent, the seeds of the autotetraploid maize hybrid Zhengdan 958 were made.

[0073] (3) Technical effects

[0074] Compared with the diploid Zhengdan 958, the autotetraploid Zhengdan 95...

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Abstract

The invention relates to a method for creating corn autotetraploid. The method comprises the following steps: treating corn filaments by adopting a conventional chromosome doubling agent and a conventional treatment method; doubling somatic chromosomes in induced organs without the need of pollination, so as to directly obtain autotetraploid seeds; then carrying out microscope examination on chromosome ploidy and screening the autotetraploid; carrying out fruitfulness breeding when the fructification percentage is relatively low; preparing autotetraploid hybrid seeds by utilizing an autotetraploid selfing line after the fructification percentage reaches the requirements. The method provided by the invention has the beneficial effects that the method is simple and feasible; seed germination, germ low-temperature treatment and seedling transplanting processes of an existing treatment method are omitted; the doubling efficiency is improved, and the problem that a chromosome ploidy chimerarepels tetraploid cells due to competitive advantages of diploid cells in a somatic cytotropism development process is avoided, so that the doubling efficiency is improved; the yield and quality areimproved and the purity is easy to ensure.

Description

Technical field [0001] The invention belongs to the technical field of corn breeding, in particular to the homologous quadruple species of sweet waxy corn and silage corn. Background technique [0002] The corn varieties produced are diploid plants. In order to meet new demands, people have adopted the method of artificially doubling chromosomes to create autotetraploid maize. Compared with diploid maize, autotetraploid maize has the characteristics of tall plants, luxuriant stems and leaves, large grains and high yield. Therefore, the homologous quadruple species can be used as a breeding way to improve the biological yield of silage corn and to improve the quality and yield of sweet corn, waxy corn and ordinary corn. [0003] In maize breeding research at home and abroad, autotetraploid is used as a way to innovate germplasm and breed new tetraploid maize varieties to increase grain and vegetative yield, increase grain weight and nutritional value. In the prior art, the method...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 史振声孙淑凤王宏伟钟雪梅王志斌张喜华
Owner SHENYANG JINSEGU SPECIAL CORN
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