Plasticizer rapid test strip based on quantum dot marking and preparation method thereof

Abstract

The invention provides a plasticizer rapid test strip based on quantum dot marking. The plasticizer rapid test strip comprises a PVC backing, a sample pad, a bonding pad, a detecting line, a quality control line, a reaction pad and an absorption pad. The detection line and the quality control line are orderly arranged on the reaction pad. The detection line is coated with a plasticizer artificialantigen. The quality control line is coated with a goat anti-mouse antibody. The binding pad is coated with a quantum dot-labeled plasticizer monoclonal antibody. The invention also provides a preparation method of the plasticizer rapid test strip based on quantum dot marking. The sample pad partially covers the bonding pad so that the observation time of the detection result is prolonged. The sample pad can fully absorb the detection liquid and fully contacts and reacts with the antibody so that the error is reduced. The test strip is low in price and simple in operation and can be operated without professionals, the test is not limited by the site, the detection time is short, and the test results can be quickly obtained on the spot, and the plasticizer rapid test strip is suitable for the detection of a large number of samples.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a plasticizer quick detection test strip based on quantum dot marking and a preparation method thereof. Background technique [0002] Plasticizer (DBP), which is a kind of environmental estrogen, is one of the commonly used plasticizers. It is widely used in toys, food packaging materials, medical blood bags and hoses, vinyl floors and wallpapers, cleaners, lubricants, personal In hundreds of products like nail polish, hair sprays, soaps and shampoos, and more. The most common plasticizers are phthalates. Phthalates can interfere with endocrine, reduce sperm count, lower motility, and abnormal shape. In severe cases, it can also lead to dead sperm and testicular dysfunction. Cancer is the "culprit" that causes male reproductive problems, and even affects embryonic development, showing reproductive and developmental toxicity. Due to the harm of plasticizers to the envir...

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Problems solved by technology

CN103076445A provides a method for detecting plasticizers using nematode larvae, which solves the technical problems of complex operation, high instrument dependence and high cost in conventional plasticizer detection methods. The method provided by the invention includes the following steps: preparing cholesterol Ethanol solution, prepare phosphate buffer solution, prepare nematode medium, evenly spread Escherichia coli OP50 bacterial solution into the nematode medium and cultivate, observe the nematode mortality in L2 stage, if the nematode larval mortality rate > 10%, it indicates that the liquor to be tested Contains plasticizers

This detection method has low requirements on experimental conditions, does not require special high-precision analytical instruments and detection equipment, and is easy to operate, low in cost, and easy to master. However, the preparation of this method takes a long time and requires professionals in the laboratory. Finish

CN105588940A discloses a time-resolved fluorescent immunoassay detection kit for detecting plasticizers. The detection kit is composed of a porous coated plate, a buffer solution, a plasticizer standard, a lyophilized product of an antibody for a plasticizer, and a europium-labeled Composed of goat anti-mouse antibody, washing solution and enhancement solution, the detection method includes the following steps: preparing immunogen, preparing coating source, preparing monoclonal antibody, pretreatment and detection of samples, the detection time of the present invention is short, and the average recovery rate High, simple sample pretreatment, on-site detection, wide application, low detection cost, strong detection specificity, small intra-batch and inter-batch differences, high sensitivity, simple and fast operation, suitable for the detection of large batches of samples. The law requires professionals to operate

CN103913525A discloses a method for detecting 17 kinds of plasticizers in liquor by high-performance liquid chromatography-tandem mass spectrometry. This method combines high-performance liquid chromatography-mass spectrometry, and can directly inject liquor samples to achieve qualitative detection. At the same time, the invention patent A quantitative detection method of HPLC-MS / MS is also disclosed. This method does not need to extract or purify the liquor, and the result can be obtained quickly by direct sample injection, which saves the time for sample pretreatment and avoids the need for sample pretreatment. The introduction of plasticizers provides technical support for the formulation of detection standards for plasticizers in liquor. This detection method has high sensitivity and reliable results, but the equipment required is expensive.

[0004] Most of the above methods require complex pretreatment, expensive equipment, time-consuming and laborious testing, small amount of samples for one test, and long testing time. They are not suitable for rapid on-site testing. They can only be used as confirmation methods in a few laboratories, and cannot meet the requirements of the environment and agricultural products. The need for quick and easy chemical reagent detection

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