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Test strip for detecting 6-monoacetylmorphine as well as preparation method and application method of test strip

A technique of monoacetylmorphine and test strips, which is applied in the detection field of 6-monoacetylmorphine, can solve the problems of complicated operation, inability to realize rapid detection of large-scale samples, long time consumption, etc., and achieves simple operation and elimination of non-specific fluorescence. interference, storage simple effects

Inactive Publication Date: 2020-03-27
郑州左安检测科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing methods for detecting 6-monoacetylmorphine, which are complex in operation, time-consuming, and unable to realize the rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, Test paper strip for detecting 6-monoacetylmorphine with fast detection speed and low cost, its preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of 6-monoacetylmorphine samples

Method used

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  • Test strip for detecting 6-monoacetylmorphine as well as preparation method and application method of test strip
  • Test strip for detecting 6-monoacetylmorphine as well as preparation method and application method of test strip
  • Test strip for detecting 6-monoacetylmorphine as well as preparation method and application method of test strip

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preparation example Construction

[0043] The preparation method of the test strip for detecting 6-monoacetylmorphine mainly comprises the following steps:

[0044] 1) labeling the 6-monoacetylmorphine antibody with fluorescent microspheres, and spraying it on the conjugate release pad to prepare a conjugate release pad sprayed with the 6-monoacetylmorphine antibody labeled with fluorescent microspheres;

[0045]2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the hapten coated with 6-monoacetylmorphine- The detection line of the carrier protein conjugate and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0046] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0047] The following step-by-step detailed descripti...

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Abstract

The invention discloses a test strip for detecting 6-monoacetylmorphine as well as a preparation method and an application method of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, an antibody marked by the time-resolved fluorescent microspheres is sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T of a hapten-carrier protein conjugate and a quality control line C of a goat-anti-mouse secondary antibody. According to the invention, the antibody is labeled by time-resolved fluorescent microspheres, and an immunochromatography technology is adopted to realize the rapid immunoassay of 6-monoacetylmorphine. The invention also provides a method for detecting the 6-monoacetylmorphine in a sample by using the above test strip. The test strip and the method have the advantages of being high in sensitivity and rapid in detection,being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of 6-monoacetylmorphine samples.

Description

technical field [0001] The invention relates to the detection of 6-monoacetylmorphine, in particular to a test strip for quantitatively detecting 6-monoacetylmorphine by using a time-resolved fluorescent microsphere immunochromatography technique and a preparation method and an application method thereof. Background technique [0002] The 2018 World Drug Report, published by the United Nations Office on Drugs and Crime, provides a comprehensive overview of the supply and demand of opiates, cocaine, cannabis, amphetamine-type stimulants and new psychoactive substances and their impact on health. Heroin is well absorbed by smoking and injection, and can penetrate the blood-brain barrier in a large amount and take effect quickly. After taking heroin, the human body will produce pleasure and hallucinations, leading to dependence and even addiction, causing great harm to individuals and society. The detection of heroin has aroused widespread concern in society. After entering th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N21/64
CPCG01N21/6428G01N33/54313G01N33/558G01N33/577
Inventor 孟二娟吕小翠贾嘉胡思敏陈丽丽蔡秋念
Owner 郑州左安检测科技有限公司
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