Constant-temperature patch clamp cell recording system

Abstract

The invention discloses a constant-temperature patch clamp cell recording system which comprises a cell operating table, a cell recording tank, a perfusion device, a constant-temperature dish, a constant-temperature column and a constant-temperature machine, wherein the cell recording tank is arranged on the cell operating table; the perfusion device is connected to the cell recording tank; the constant-temperature dish is matched with the cell recording tank; the constant-temperature column is at least arranged on the perfusion device; the constant-temperature machine is connected with the constant-temperature column and the constant-temperature dish. The constant-temperature patch clamp cell recording system disclosed by the invention has the benefits that as the constant-temperature column is arranged outside a perfusion tube, when a perfusate in the perfusion tube flows through a water holding chamber of the constant-temperature column, the heat is conducted through warm water in the water holding chamber, so that the perfusate in the perfusion tube meets the experiment temperature requirements; in the experiment process, by matching of a water bah of the constant-temperature dish and the cell recording tank, the temperature stabilities of a cell and a reagent in the experiment process are ensured in a water bath manner of circulating constant-temperature water in the cellexperiment process, ohmic heating is not required, and shapes of experimental square waves cannot be interfered, so that experimental data are more realistic, more accurate and more reliable.

Description

technical field [0001] The invention relates to the technical field of medical equipment, in particular to a constant temperature patch clamp cell recording system. Background technique [0002] The patch clamp technique is a research method used to record the electrophysiological properties of ion channels on the whole cell or individual cell membranes. The purpose is to provide basic research knowledge and to study the influence of cell electrical properties or small molecule drugs on the properties of ion channels on cell membranes during the development of new drugs. Provide a testing platform for the development of targeted drugs. The traditional cell culture patch clamp system is manually operated. After the experimenter obtains metastatic cells (such as cardiomyocytes and neurons), he raises the research object cells on glass slides, and manually moves the recording electrode to place on the cell body and Pressed onto the cell membrane, the resistance of the recordin...

AI Technical Summary

Problems solved by technology

Although the cell recording tanks currently on the market seem simple, most of them are imported products and are expensive. In addition, the existing cell recording tanks also have the following disadvantages: 1. The material is metal, which is easy to conduct electricity. Cause interference to the experiment and affect the shape of the experimental square wave; 2. The perfusion port of the existing cell recording tank is low, almost in the same plane as the cell fluid, and the cells are easily washed away when the perfusate is introduced into the cell recording tank; 3. The liquid outlet level Low, when the perfusate is pumped, it is difficult to grasp the balance of the perfusate in and out, and the extracellular fluid is easily drained and the cells are lost; 4. The reference electrode is placed too large, and the reference electrode is easy to shake when moving the glass stage , which affects the square wave shape of the experiment; 5. Cell recording tanks with heating devices or heating chips are expensive and easy to burn dry

[0004] In view of this, the designer aimed at the many defects and inconveniences caused by the imperfect design of the above-mentioned patch clamp cell recording system, and deeply conceived, and actively researched and improved the trial and developed and designed the present invention

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