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A method for inducing human adipose stem cells to differentiate into Leydig cells with small molecules

A technology of Leydig cells and human adipose stem cells, applied in the field of biotechnology, can solve unpublished problems and achieve the effects of improving safety, good repeatability, and avoiding excessive induction

Active Publication Date: 2021-07-27
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
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  • Claims
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Problems solved by technology

After years of research, adipose stem cells (ADSCs) can be induced to differentiate into various cells, but the technology of inducing human adipose stem cells to differentiate into Leydig cells through small molecules has not been disclosed; therefore, this patent will be based on the use of human fat Stem cells (hADSCs), using small molecules instead of gene transfer methods to induce the differentiation of adipose-derived stem cells (ADSCs) into functional mature Leydig cells (ALCs), for future patients using autologous adipose-derived stem cells (ADSCs) to differentiate testicular Leydig cells ( ALC) transplantation provides scientific basis for the treatment of androgen deficiency

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  • A method for inducing human adipose stem cells to differentiate into Leydig cells with small molecules
  • A method for inducing human adipose stem cells to differentiate into Leydig cells with small molecules
  • A method for inducing human adipose stem cells to differentiate into Leydig cells with small molecules

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Embodiment Construction

[0019] The present invention is not limited to the following specific embodiments. Those skilled in the art can implement the present invention in various other specific embodiments according to the disclosed content of the present invention, or make simple changes or All changes fall within the protection scope of the present invention.

[0020] Specific embodiments of the present invention such as Figure 1-4 Shown is the method for small molecule induced fat stem cells to differentiate into Leydig cells, which includes the following steps,

[0021] ① Obtaining human adipose stem cells: In strict accordance with the requirements of aseptic operation, the fat extracted from the abdomen of six women (or extracted from the existing fat bank) was sequentially transferred into 50mL centrifuge tubes in the ultra-clean workbench, and stood still for 10 After the interstitial fluid and fat are separated within 1 minute, use a Pasteur tube to remove the interstitial fluid at the botto...

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Abstract

The invention discloses a method for fully utilizing small molecules to induce human fat stem cells to differentiate into Leydig cells (LCs). The steps of the method are as follows. First, human adipose stem cells are isolated and identified; then, a specific medium is used to induce the differentiation of human adipose stem cells, and a certain amount of small molecules are added to the medium at different time points, including: SAG, 22OHC, Li, PDGF‑ AA, FGF2, Androgen, and LH promote differentiation; finally, the non-testis Leydig cells (ALCs)-like cells are manually removed to enrich the differentiation target cells (ADSC‑ALCs), and the identification is completed. Induced according to the method of the present invention, human adipose stem cells can be successfully differentiated into Leydig cells of the testis, thereby providing a cell source for the treatment of testosterone deficiency by cell transplantation.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, in particular to a method for fully utilizing small molecules to induce human adipose stem cells to differentiate into Leydig cells. Background technique [0002] Male reproductive health is inseparable from the functioning of the reproductive system. Currently, the only treatment for androgen deficiency is androgen replacement therapy. However, long-term androgen therapy can cause complications such as liver and kidney damage, decreased immunity, water and sodium retention, and is not regulated by its own circadian rhythm. At present, a hot spot in medical research is cell replacement therapy. Although Leydig cells (LCs) transplantation can avoid some complications caused by exogenous androgen replacement therapy, insufficient sources of Leydig cells (LCs) and allogeneic transplantation may lead to host immune rejection The reaction limits the clinical application of Leydig cells (LCs)....

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/0683C12N2500/12C12N2500/35C12N2500/40C12N2501/115C12N2501/135C12N2501/31C12N2501/33C12N2501/39C12N2501/999C12N2506/1384
Inventor 郭晓令葛仁山李超林振坤李晓珩洪婷婷陈显武陈勇
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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