A method for inducing human adipose stem cells to differentiate into Leydig cells with small molecules

Abstract

The invention discloses a method for fully utilizing small molecules to induce human fat stem cells to differentiate into Leydig cells (LCs). The steps of the method are as follows. First, human adipose stem cells are isolated and identified; then, a specific medium is used to induce the differentiation of human adipose stem cells, and a certain amount of small molecules are added to the medium at different time points, including: SAG, 22OHC, Li, PDGF‑ AA, FGF2, Androgen, and LH promote differentiation; finally, the non-testis Leydig cells (ALCs)-like cells are manually removed to enrich the differentiation target cells (ADSC‑ALCs), and the identification is completed. Induced according to the method of the present invention, human adipose stem cells can be successfully differentiated into Leydig cells of the testis, thereby providing a cell source for the treatment of testosterone deficiency by cell transplantation.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, in particular to a method for fully utilizing small molecules to induce human adipose stem cells to differentiate into Leydig cells. Background technique [0002] Male reproductive health is inseparable from the functioning of the reproductive system. Currently, the only treatment for androgen deficiency is androgen replacement therapy. However, long-term androgen therapy can cause complications such as liver and kidney damage, decreased immunity, water and sodium retention, and is not regulated by its own circadian rhythm. At present, a hot spot in medical research is cell replacement therapy. Although Leydig cells (LCs) transplantation can avoid some complications caused by exogenous androgen replacement therapy, insufficient sources of Leydig cells (LCs) and allogeneic transplantation may lead to host immune rejection The reaction limits the clinical application of Leydig cells (LCs)....

AI Technical Summary

Problems solved by technology

After years of research, adipose stem cells (ADSCs) can be induced to differentiate into various cells, but the technology of inducing human adipose stem cells to differentiate into Leydig cells through small molecules has not been disclosed; therefore, this patent will be based on the use of human fat Stem cells (hADSCs), using small molecules instead of gene transfer methods to induce the differentiation of adipose-derived stem cells (ADSCs) into functional mature Leydig cells (ALCs), for future patients using autologous adipose-derived stem cells (ADSCs) to differentiate testicular Leydig cells ( ALC) transplantation provides scientific basis for the treatment of androgen deficiency

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