Method for promoting stable and rapid implementation of denitrification process through microorganism co-culture

A technology of co-cultivation and denitrification, applied in the field of biological denitrification, can solve problems such as lag, and achieve the effects of low cost, obvious removal effect, and no accumulation of nitrite.

Inactive Publication Date: 2018-09-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the hysteresis stage of the denitrification process under the change of external conditions in the denitrification process of sewage treatment

Method used

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  • Method for promoting stable and rapid implementation of denitrification process through microorganism co-culture
  • Method for promoting stable and rapid implementation of denitrification process through microorganism co-culture
  • Method for promoting stable and rapid implementation of denitrification process through microorganism co-culture

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Experimental program
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Effect test

Embodiment 1

[0026] Example 1: A method for co-cultivating microorganisms to promote the denitrification process to proceed stably and rapidly

[0027] In view of the change of oxygen-containing conditions and substrates, denitrifying bacteria need a period of time to adapt to the new environment, and the denitrification process lags behind. Aiming at this problem, the present invention proposes the co-cultivation technology of domesticated denitrifying bacteria and G.sulfurrenducens to solve the problem, and utilizes microbiology and microscope imaging technology to conduct mechanism analysis to realize the stable and rapid denitrification process. The specific steps of the co-culture method are as follows:

[0028] 1) Construction of co-cultivation system;

[0029] The denitrifying bacteria were obtained by domesticating the sludge with methanol under aerobic conditions in advance, and the composition of the domestication medium was: Na 2 HPO 4 0.8g / L, CaCl 2 0.1g / L,FeSO 4 ·7H 2 O ...

Embodiment 2

[0036] Example 2: Under different carbon-nitrogen ratios, a method for the co-cultivation of microorganisms to promote the stable and rapid denitrification process

[0037] In view of the uncertainty of the carbon-nitrogen ratio of sewage under different conditions, in view of this problem, it is proposed that the co-cultivation of denitrifying bacteria and G.sulfurrenducens under different carbon-nitrogen ratios can promote the stable and rapid denitrification process.

[0038] The specific steps of the co-culture method are as follows:

[0039] 1) Construction of co-cultivation system;

[0040]The denitrifying bacteria were obtained by domesticating the sludge with methanol under aerobic conditions in advance, and the composition of the domestication medium was: Na 2 HPO 4 0.8g / L, CaCl 2 0.1g / L,FeSO 4 ·7H 2 O 0.07g / L, MgSO 4 ·7H 2 O 0.1g / L. Methanol 20 millimoles, nitrate 3-9 millimoles, the domesticated communities are mainly Delftia, Methyllobacillus, Diaphorobacter...

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Abstract

The invention discloses a method for promoting stable and rapid implementation of a denitrification process through microorganism co-culture. The co-culture is realized by acclimatizing to obtain denitrifying bacteria, and co-inoculating in an anaerobic bottle culture medium with G.sulfurrenducens. An electron donor and an acceptor are sodium acetate and nitrate respectively. OD 600 is 0.6 after inoculating, N2 / CO2 (80:20) is used for performing aeration to remove oxygen, and anaerobic culture is performed in 30 DEG C. The method is capable of removing the nitrate in the culture medium of which a carbon nitrogen ratio is 1-9, and guaranteeing the validity in the different carbon nitrogen ratios. The beneficial effect is that syntrophism growth between microorganisms is used for promoting the denitrification, the cost is low, the removal effect of the nitrate is apparent, and nitrite is not accumulated.

Description

technical field [0001] The invention relates to the technical field of biological denitrification in wastewater treatment, in particular to a technique for co-cultivating denitrifying bacteria and Geobacter sulfurrenducens under changing external conditions to promote the stable and rapid denitrification process. Background technique [0002] With the rapid development of economy and society, the problem of water pollution is becoming more and more serious. Nitrogen-containing compounds will cause eutrophication of the water environment and pose a potential hazard to human health. The removal of nitrate in wastewater treatment has always attracted much attention. Chemical denitrification, reverse osmosis, electrodialysis, catalytic denitrification and biological denitrification are commonly used methods. Among them, the biological denitrification process has a wide range of environmental protection products due to its low cost in the removal process. Application prospect. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34
CPCC02F3/28C02F3/34C02F2305/06
Inventor 王鑫万雨轩
Owner NANKAI UNIV
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